Evaluation of a saliva test kit for feline leukemia virus antigen

1996 ◽  
Vol 32 (5) ◽  
pp. 397-400
Author(s):  
SD Babyak ◽  
MG Groves ◽  
DS Dimski ◽  
J Taboada
Keyword(s):  
Leukemia Virus ◽  
Virus Antigen ◽  
Elisa Test ◽  
Feline Leukemia Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Kit ◽  
Test Kit ◽  
Blood Smears ◽  
Indirect Immunofluorescent ◽  
Saliva Test

An enzyme-linked immunosorbent assay (ELISA) test kit for the detection of feline leukemia virus (FeLV) antigen in saliva was evaluated in 150 cats. Saliva and blood samples from all cats were tested for FeLV using the saliva ELISA kit and a plasma ELISA kit, respectively. These results were compared with indirect immunofluorescent antibody (IFA) testing of blood smears also obtained from each cat. The proportion of cats that tested positive were 10%, 7%, and 8% for each test, respectively. Using the IFA test as the gold standard, the saliva FeLV test had a sensitivity of 91.7% and specificity of 97.1%, while the plasma ELISA test had a sensitivity of 91.7% and specificity of 100%.

scholarly journals Evaluation of Vaccination Strategy Against Rabies in Hong Kong Macaques

2021 ◽  
Author(s):  
Omid Nekouei ◽  
Paolo Martelli ◽  
Sophie St-Hilaire ◽  
Hui Suk Wai ◽  
Karthiyani Krishnasamy ◽  
...  
Keyword(s):  
Hong Kong ◽  
Vaccination Strategy ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Response ◽  
Test Kit ◽  
In The Wild ◽  
The Government ◽  
Specific Association ◽  
Antibody Levels

Rabies is a fatal zoonotic disease that can affect all mammals. Following the directives of the rabies ordinance of the Government of Hong Kong, all wild macaques captured under an ongoing sterilization program (since 2000) were vaccinated against rabies. The main objective of this study was to assess the serological response to rabies vaccination in the population of Hong Kong macaques. An inactivated rabies vaccine was subcutaneously administered to captured macaques under anesthesia. In a 2015 field survey, blood samples from the animals were collected and stored in -80℃ freezer. In July 2021, all frozen sera from vaccinated animals were prepared and tested for antibodies against rabies virus using a commercial enzyme-linked immunosorbent assay (ELISA) test. The test results were dichotomized at the recommended cut-off point of the test kit. Sixty-five samples from the vaccinated macaques were available for this study. All of these animals had received at least one dose of vaccine (1 vaccination) between 2008 and 2015. The interval between the 1 vaccination and blood sampling dates ranged from 21 to 2,779 days. Only five of the 65 macaques had a second vaccination record at the time of sampling; all five had high antibody levels. Among the remaining macaques, 77% (46/60) were positive for rabies antibodies. No specific association was observed between the post-vaccination period and the antibody titer of these macaques and no adverse reactions to vaccination were reported. The current vaccination strategy in Hong Kong macaques appears to effectively elicit rabies antibodies in a high proportion of macaque populations in the wild (78-87%). However, reaching the precise level of protection against a potential challenge with the virus should further be investigated.

scholarly journals Saliva as a source of feline leukemia virus antigen for diagnosis of disease.

1987 ◽  
Vol 25 (7) ◽  
pp. 1320-1322 ◽  
Author(s):  
M G Lewis ◽  
K A Wright ◽  
L J Lafrado ◽  
P J Shanker ◽  
N E Palumbo ◽  
...  
Keyword(s):  
Leukemia Virus ◽  
Virus Antigen ◽  
Feline Leukemia Virus

Feline immunodeficiency virus diagnosis after vaccination

2004 ◽  
Vol 5 (2) ◽  
pp. 327-330 ◽  
Author(s):  
Philip R. Andersen ◽  
Phyllis Tyrrell
Keyword(s):  
Western Blot ◽  
Feline Immunodeficiency Virus ◽  
American Association ◽  
Leukemia Virus ◽  
Virus Antigen ◽  
Feline Leukemia Virus ◽  
Antibody Detection ◽  
Predictive Values ◽  
Virus Diagnosis ◽  
Immunodeficiency Virus

AbstractPrior to the widespread use of vaccination for the control of feline immunodeficiency virus (FIV) infection, diagnosis was made by the detection of antibodies against FIV. A number of commercial animal side tests perform quite well for this determination, with positive predictive values between 91 and 100% and negative predictive values between 96 and 100%. Furthermore, results of these tests could be confirmed by western blot analysis of FIV test-positive sera. Currently, a killed whole virus FIV vaccine has been made available to practitioners. Vaccinated cats seroconvert by ELISA and western blot, making presently available diagnostic tests, which rely on antibody detection, useless in cats after vaccination. The advisory panels of the American Association of Feline Practitioners and Academy of Feline Medicine both recommend testing for feline leukemia virus antigen and FIV antibody before vaccination.

Pelger-Huët Anomaly in Cats

1985 ◽  
Vol 22 (4) ◽  
pp. 370-374 ◽  
Author(s):  
K. S. Latimer ◽  
P. M. Rakich ◽  
D. F. Thompson
Keyword(s):  
Bone Marrow ◽  
Virus Infection ◽  
Autosomal Dominant ◽  
Leukemia Virus ◽  
Young Male ◽  
Clinical Disease ◽  
Feline Leukemia Virus ◽  
Genetic Transmission ◽  
Blood Smears ◽  
Nuclear Segmentation

Pelger-Huët anomaly was diagnosed in a young male cat on the basis of persistent nuclear hyposegmentation of blood granulocytes, absence of clinical disease or feline leukemia virus infection, and demonstration of genetic transmission of the anomaly. Neutrophils, eosinophils, basophils, and monocytes had significantly less nuclear segmentation when compared to similar leukocyte types from a control cat. On smears of bone marrow, megakaryocytes also appeared hyposegmented. A test mating with an unaffected queen produced a litter of five kittens (three males, two females). One male and one female kitten had the Pelger-Huët trait on examination of blood smears. Autosomal dominant transmission of this anomaly is suspected based on these findings.

Prevalence of Potentially Pathogenic Enteric Organisms in Clinically Healthy Kittens in the UK

2009 ◽  
Vol 11 (8) ◽  
pp. 655-662 ◽  
Author(s):  
Adam G. Gow ◽  
Deborah J. Gow ◽  
Edward J. Hall ◽  
Debra Langton ◽  
Chris Clarke ◽  
...  
Keyword(s):  
Gastrointestinal Disease ◽  
Incidental Finding ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Negative Results ◽  
Test Kit ◽  
Faecal Samples ◽  
High Prevalence ◽  
Campylobacter Species ◽  
The Uk

Faecal samples were collected from 57 clinically healthy kittens presented for initial vaccination, in the UK. Routine bacteriological examination identified Salmonella species in one and Campylobacter species in five samples. Polymerase chain reaction (PCR) detected the presence of Campylobacter species in a further four samples. Routine parasitological examination revealed Toxocara species ova in nine (including four kittens stated to have been administered an anthelmintic) and Isospora species in four samples. No Giardia or Cryptosporidium species were detected by routine methods. A Giardia species enzyme-linked immunosorbent assay (ELISA) test kit designed for use in cats was positive in three kittens. A similar test kit designed for use in humans was negative in all samples and produced negative results even when known positive samples were tested. Potentially pathogenic enteric organisms were detected in 19 kittens by routine methods and 26 (prevalence 45%) by all methods. The high prevalence in asymptomatic kittens highlights the possibility that the detection of these organisms in kittens with gastrointestinal disease may be an incidental finding.

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