STUDY OF THE BIOCOMPATIBILITY OF MUTANT STRAINS OF ROOT NODULE AND PGPR BACTERIA, PROMISING AS A BASIS OF MICROBIAL PREPARATIONS

2021 ◽  
Author(s):  
О.Н. ШЕМШУРА ◽  
Ж.Б. СУЛЕЙМЕНОВА ◽  
Ж.К. РАХМЕТОВА ◽  
Ж.Н. ШЕМШЕЕВА ◽  
Э.Т. ИСМАИЛОВА
Keyword(s):  
Bacillus Subtilis ◽  
Pseudomonas Putida ◽  
Mung Bean ◽  
Root Nodule ◽  
Nitrogen Fixing ◽  
Nodule Bacteria ◽  
Biological Product ◽  
Bean Plants ◽  
Combined Use ◽  
Mutant Strains

В статье приведены результаты исследования биосовместимости мутантных штаммов клубеньковых и PGPR бактерий (ризобактерий) с целью их совместного применения для культур маша и фасоли. По результатам проведенных исследований определены штаммы, проявившие контактную прогрессию и нейтралитет - Pseudomonas putidaМ-1 и Phyllobacterium sp. 35М; штаммы Bacillus subtilis М-2 и Chryseobacterium rhizoplanae 1М оказались наиболее перспективными в отношении совместного культивирования.Таким образом, подобраны консорциумы на основе мутантных штаммов азотфиксирующих и ростостимулирующих бактерий Pseudomonas putida М-1 и Chryseobacterium rhizoplanae для растений маша и Bacillus subtilis М-2 и Phyllobacterium sp. 35М - для растений фасоли. Полученные результаты открывают возможность комбинирования мутантных штаммов PGPR с ростостимулирующей активностью (Pseudomonas putida М-1, Bacillus subtilis М-2) и клубеньковыхбактерий (Phyllobacterium sp. 35М, Chryseobacterium rhizoplanae) с азотфиксирующей активностью с целью получения на их основе биопрепарата с сочетанными свойствами. The article presents the results of a study of the biocompatibility of mutant strains of nodule and PGPR bacteria with the aim of their combined use for mung bean and beans. According to the results of the studies, the strains that showed contact progression and neutrality were identified - Pseudomonas putida M-1 and Phyllobacterium sp. 35M; strains Bacillus subtilis M-2 and Chryseobacterium rhizoplanae 1M proved to be the most promising for co-cultivation. Thus, consortia were selected based on mutant strains of nitrogen-fixing and growth-stimulating bacteria Pseudomonas putida M-1 and Chryseobacterium rhizoplanae for mung bean and Bacillus subtilis M-2 and Phyllobacterium sp.35M for bean plants. The results obtained open up the possibility of combining mutant PGPR strains with growth-stimulating activity (Pseudomonas putida M-1, Bacillus subtilis M-2) and nodule bacteria with nitrogen-fixing activity (Phyllobacterium sp. 35M, Chryseobacterium rhizoplanae) in order to obtain a biological product with combined properties on their basis.

Distribution, frequency of occurrence and symbiotic properties of the Australian native legume Trigonella suavissima Lindl. and its associated root-nodule bacteria

2010 ◽  
Vol 32 (4) ◽  
pp. 395 ◽  
Author(s):  
J. Brockwell ◽  
Catherine M. Evans ◽  
Alison M. Bowman ◽  
Alison McInnes
Keyword(s):  
Root Nodule ◽  
Clay Soils ◽  
Frequency Of Occurrence ◽  
Nitrogen Fixing ◽  
Nodule Bacteria ◽  
River Systems ◽  
Root Nodule Bacteria ◽  
North East ◽  
Inland River ◽  
Native Legume

Trigonella suavissima Lindl. is an Australian native legume belonging to the tribe Trifolieae. It is an ephemeral species that is widely distributed in the arid interior of the continent where it occurs, following periodic inundation, on clay soils of the watercourse country of the Channel Country (far-western Queensland, north-east South Australia and north-western New South Wales). T. suavissima is the only member of its tribe that is endemic to Australia. Likewise, its root-nodule bacteria (Sinorhizobium sp.) may be the only member of its taxonomic group (S. meliloti, S. medicae) that is an Australian native. The distribution and frequency of occurrence of T. suavissima and the size of soil populations (density) of Sinorhizobium were monitored at 64 locations along inland river systems of the Channel Country. Measurements were made of (i) the nitrogen-fixing effectiveness of the symbioses between T. suavissima and strains of its homologous Sinorhizobium and (ii) the nitrogen-fixing effectiveness of the symbioses between legumes symbiotically related to T. suavissima and diverse strains of Sinorhizobium. It was concluded that the distribution and frequency of occurrence of T. suavissima is soil related. The species is most widespread on fine-textured clay soils with deep, self-mulching surfaces and high moisture-holding capacity. By contrast, the occurrence of T. suavissima is sporadic in the upper reaches of the inland river systems where the soils are poorly structured clays with lower moisture-holding capacity. Sinorhizobium is most abundant where the plant is most common. The nitrogen-fixing symbioses between T. suavissima and strains of Sinorhizobium isolated from soils across the region were consistently effective and often highly effective. Some of these strains fixed a little nitrogen with lucerne (Medicago sativa L.). T. suavissima also had some symbiotic (nitrogen-fixing) affinity with an exotic Trigonella (T. arabica Del.). The economic value of T. suavissima (and its symbiosis with Sinorhizobium) to the beef industry in the Channel Country is discussed.

Nitrogen fixation in legume root nodules: biochemical studies with soybean

1969 ◽  
Vol 172 (1029) ◽  
pp. 401-416 ◽  
Keyword(s):  
Nitrogen Fixation ◽  
Root Nodule ◽  
Root Nodules ◽  
Reducing Power ◽  
Nitrogen Fixing ◽  
Nodule Bacteria ◽  
Stages Of Growth ◽  
Host Cytoplasm ◽  
Photosynthetic Product ◽  
Power And Energy

It is now clear from studies with soybean root nodules that the nitrogen fixing activity resides in the bacteroids which are the symbiotic form of the root nodule bacteria. These develop as a result of a complex series of changes in metabolism and structure which occur in the bacteria during the final stages of growth within membrane-enclosed vesicles in the host cytoplasm. Nitrogenase appears when these changes are complete. The primary product of nitrogen fixation is NH 3 , which in intact nodules, is rapidly transformed into α -amino compounds which are used by the host plant. In suspensions of bacteroids and in cell-free extracts prepared from them, the reaction terminates in NH 3 , which is released into the medium. Free O 2 , which is required for the production of energy for nitrogen fixation by nodules and by bacteroid suspensions, also causes inactivation of the nitrogen fixing system and exerts important kinetic influences upon the reaction. Reducing power and energy for the reduction of N 2 to NH 3 is provided by a photosynthetic product from the host in nodules; in bacteroid suspensions, a substrate such as succinate is required. In cell-free extracts, requirements for energy and reductant are met by ATP and dithionite. The natural reductant has not yet been identified. A schematic representation of various factors which affect nitrogen fixation in nodules, bacteroid suspensions and cell-free extracts is presented.

scholarly journals Tetraponeraants have gut symbionts related to nitrogen–fixing root–nodule bacteria

2002 ◽  
Vol 269 (1504) ◽  
pp. 2023-2027 ◽  
Author(s):  
Steven van Borm ◽  
Alfred Buschinger ◽  
Jacobus J. Boomsma ◽  
Johan Billen
Keyword(s):  
Root Nodule ◽  
Nitrogen Fixing ◽  
Nodule Bacteria ◽  
Root Nodule Bacteria ◽  
Gut Symbionts

scholarly journals Characterization of two lipopolysaccharide types isolated from Rhizobium galegae.

1997 ◽  
Vol 44 (4) ◽  
pp. 819-825 ◽  
Author(s):  
L A Räsänen ◽  
R Russa ◽  
T Urbanik ◽  
A Choma ◽  
H Mayer ◽  
...  
Keyword(s):  
Root Nodule ◽  
Gel Filtration ◽  
Nitrogen Fixing ◽  
Nodule Bacteria ◽  
Root Nodule Bacteria ◽  
Antigenic Polysaccharide ◽  
Phenol Water

Lipopolysaccharides (LPS) of Rhizobium galegae, a symbiotically nitrogen-fixing species of root-nodule bacteria, were isolated by the phenol-water method from strain HAMBI 1461, the LPS of which resembled enterobacterial smooth type LPS, and from strains HAMBI 1174 and HAMBI 1208, the LPSs of which resembled rough type LPS. The results of PAGE analysis of LPSs, Bio-Gel P2 gel filtration of polysaccharide fractions and the presence of deoxysugars and 4-O-methyl-deoxysugar both in the rough and smooth LPSs suggested that rough LPS contained a short O-antigenic polysaccharide for which we propose the name short O-chain LPS. Accordingly, the smooth LPS is called long O-chain LPS. Despite of the differences in the structure of LPS of R. galegae, all strains were equally effective in nodulating their hosts. The short O-chain LPS of R. galegae showed many features similar to those of phylogenetically related agrobacteria.

Comigration of root nodule bacteria and bean plants to new habitats: Coevolution mechanisms and practical importance

2013 ◽  
Vol 49 (3) ◽  
pp. 209-214 ◽  
Author(s):  
N. A. Provorov ◽  
V. A. Zhukov ◽  
O. N. Kurchak ◽  
O. P. Onishchuk ◽  
E. E. Andronov ◽  
...  
Keyword(s):  
Root Nodule ◽  
Practical Importance ◽  
Nodule Bacteria ◽  
Root Nodule Bacteria ◽  
Bean Plants ◽  
New Habitats

scholarly journals Association of nitrogen-fixing microorganisms in the surface of nodules in wild perennial leguminous plants Onobrychis transcaucasica and Onobrychis chorassanica

2020 ◽  
Author(s):  
B. R. Umarov
Keyword(s):  
Genetic Analysis ◽  
Central Asia ◽  
Root Nodule ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Arid Zones ◽  
Nitrogen Fixing ◽  
Nodule Bacteria ◽  
Leguminous Plants ◽  
Root Nodule Bacteria

The results of molecular genetic analysis root nodule bacteria wild leguminous plants germinating in the Arid zones Central Asia can penetrate into various nitrogen-fixing microorganisms. Bacteria of plants Onobrychis transcaucasica and Onobrychis chorossanica origin are found bacteria in the class Alphaproteobacteria and some nitrogen-fixing bacteria which we are write were in the class of Betaproteobacteria.

scholarly journals The combined use of Bacillus subtilis-based probiotic and anticoccidial herb had a better anti-Eimeria tenella efficiency

2021 ◽  
pp. 100181
Author(s):  
Yunqiao Yang ◽  
Fareed Uddin Memon ◽  
Kaiyuan Hao ◽  
Mingsheng Jiang ◽  
Lei Guo ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Eimeria Tenella ◽  
Combined Use
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