scholarly journals Effects of Stimulation and Blockade of Receptor on Depression-Like Behavior in Ovariectomized Female Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Julia Fedotova
Keyword(s):  
Receptor Antagonist ◽  
Receptor Agonist ◽  
Low Dose ◽  
Forced Swimming Test ◽  
Forced Swimming ◽  
Female Rats ◽  
Ovx Rats ◽  
Wistar Strain ◽  
Immobility Time ◽  
Swimming Test

The aim of the present study was to explore the hedonic effects of D2 receptor agonist, quinpirole and D2 receptor antagonist, and sulpiride alone or in combination with a low dose of 17β-E2-estradiol (17β-E2) in the adult ovariectomized female rats (OVX). OVX rats of Wistar strain were used in all experiments. Two weeks after surgery rats were chronically treated with vehicle, a low dose of 17β-E2 (5.0 μg/rat), quinpirole (0.1 mg/kg), sulpiride (10.0 mg/kg), quinpirole plus 17β-E2, or sulpiride plus 17β-E2 for 14 days before the forced swimming test. We found that sulpiride significantly decreased immobility time in the OVX females. A combination of sulpiride with a low dose of 17β-E2 induced more profound decrease of immobility time in the OVX rats compared to the rats treated with sulpiride alone. On the contrary, quinpirole failed to modify depression-like behavior in the OVX rats. In addition, quinpirole significantly blocked the antidepressant-like effect of 17β-E2 in OVX rats. Thus, the D2 receptor antagonist sulpiride alone or in combination with a low dose of 17β-E2 exerted antidepressant-like effect in OVX female rats, while the D2 receptor agonist quinpirole produced depressant-like profile on OVX rats.

scholarly journals Antidepressant Effect ofTetragonia tetragonoides (Pall.) KuntzeExtract on Serotonin Turnover

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Hyun Yang ◽  
Hye Jin Kim ◽  
Eui-Ju Hong ◽  
Bo-Jeong Pyun ◽  
Byung-Seob Ko ◽  
...  
Keyword(s):  
Serum Level ◽  
Forced Swimming Test ◽  
Forced Swimming ◽  
Female Rats ◽  
Serotonin Level ◽  
Mrna Levels ◽  
Dependent Manner ◽  
Ovx Rats ◽  
Immobility Time ◽  
Swimming Test

Tetragonia tetragonoides (Pall.) Kuntze(TTK) is a groundcover found along coastal areas of the Korean peninsula. TTK is traditionally used to improve women’s health and treat gastrointestinal diseases. Use of herbal medicines in the treatment of mood disorders has recently been suggested as an alternative therapeutic strategy. In the present study, we determined that consumption of TTK extract ameliorated progression of depressive-like symptoms in ovariectomized (OVX) rats and further examined the mechanisms involved, i.e., synthesis, release, and reuptake(s) of serotonin (also known as 5-HT). We assessed the mRNA expression levels of tryptophan hydroxylases (TPH-1 and TPH-2) and serotonin transporter (SERT) as well as the reuptake activity of serotonin in RBL-2H3 cells. We also determined whether or not TTK extract regulates the serum level of serotonin and improves depressive-like symptoms in 0.5, 1, and 2% TTK-fed OVX female rats in a forced swimming test. Our results show that the mRNA levels of TPH-1 and SERT were significantly reduced, whereas the mRNA level of TPH-2 was dose-dependently elevated by TTK (50 and 100μg/mL) in RBL-2H3 cells. TTK significantly inhibited LPS- (lipopolysaccharide-) induced serotonin uptake in RBL-2H3 cells in a dose-dependent manner. The serum level(s) of serotonin was elevated by 1% and 2% TTK treatment in OVX female rats. Moreover, immobility time in the forced swimming test was reduced by 1% and 2% TTK treatment but not altered by 0.5% TTK treatment in OVX female rats. Taken together, these results indicate that TTK may significantly inhibit depressive-like symptoms due to upregulation of serotonin level(s) and regulation of serotonin reuptake activity. Thus, TTK may exert beneficial effects on depression during pre- or/and postmenopausal periodsviamodulation of serotonin synthesis and metabolism.

Repeated forced-swimming test in intact female rats

2018 ◽  
Vol 29 (6) ◽  
pp. 509-518 ◽  
Author(s):  
Karolina Domingues ◽  
Inaê Spezia ◽  
Lais C. Theindl ◽  
Patrick R. Suman ◽  
Fernanda B. Lima ◽  
...  
Keyword(s):  
Forced Swimming Test ◽  
Forced Swimming ◽  
Female Rats ◽  
Intact Female ◽  
Swimming Test

Modifications of depression-like behavior in the adult ovariectomized female rats treated with different doses of cholecalciferol

2017 ◽  
Vol 41 (S1) ◽  
pp. S527-S527
Author(s):  
J. Fedotova ◽  
T. Dudnichenko
Keyword(s):  
Preclinical Study ◽  
Female Rats ◽  
High Dose ◽  
Russian Science ◽  
Once Daily ◽  
Ovx Rats ◽  
Immobility Time ◽  
Reported Study ◽  
Swimming Test ◽  
Competing Interest

The aim of the preclinical study was to examine the effects of chronic the effects of chronic cholecalciferol administration (1.0, 2.5 or 5.0 mg/kg/day, s.c., once daily, for 14 days) on depression-like behavior following ovariectomy in rats. Cholecalciferol was administered to the ovariectomized (OVX) rats and OVX rats treated with 17β-estradiol (17β-E2, 0.5 μg/rat, s.c., once daily, for 14 days). Depression-like behavior was assessed in the forced swimming test (FST) and the spontaneous locomotor activity was assessed using the open field test (OFT). Treatment with cholecalciferol in high dose (5.0 mg/kg/day, s.c.) significantly decreased immobility time of OVX rats in the FST. Co-administration of cholecalciferol in high dose with 17β-E2 exerted a markedly synergistic antidepressant-like effect in the OVX rats on the same model of depression-like behavior testing. Cholecalciferol in high dose administered alone or together with 17β-E2 significantly enhanced frequency of grooming of the OVX rats in the OFT. Moreover, cholecalciferol in high dose administered alone or together with 17β-E2 significantly decreased the elevated corticosterone levels in the blood serum of OVX rats following the FST. These results indicate that cholecalciferol in high dose has a marked antidepressant-like effect in the adult female rats with low levels of estrogen. The data also indicate that the combination of cholecalciferol in high dose and 17β-E2 is more effective than 17β-E2 alone in OVX rats inducing a more profound antidepressant-like effect in the FST.Russian Science Foundation (RSF) funded the reported study accordingly to the research project № 16-15-10053.Disclosure of interestThe authors have not supplied their declaration of competing interest.

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