scholarly journals QUANTITATIVE DETERMINATION OF SULPHATE REDUCING BACTERIA AND HYDROGEN SULPHIDE IN HUMAN SALIVA

2021 ◽  
Vol 80 (1) ◽  
pp. 35-42
Author(s):  
Qasem M. Abu Shaqra ◽  
Rania M. Al Groom ◽  
Ahmad Al-Gabbiesh
Keyword(s):  
Statistical Significance ◽  
Diagnostic Value ◽  
Human Saliva ◽  
Ion Concentration ◽  
Oral Microbiota ◽  
Oral Manifestations ◽  
Sulphate Reducing Bacteria ◽  
Oral Conditions ◽  
Reducing Bacteria

"Objectives: Sulphate Reducing Bacteria (SRB) are part of the normal oral microbiota. They have been frequently detected in the oral cavity of individuals with and without oral manifestations. The aim of this investigation was to determine possible correlation between SRB count and their activity in saliva versus some oral health manifestations. Methods: A total of 64 saliva samples were collected from healthy individuals, and patients with several oral manifestations. Detection and count of SRB were performed using documented methods, while sulphide ion concentration which is the metabolic end product of these bacteria was measured using a silver electrode connected to a millivolt meter. Results: SRB were detected in 35.9% of saliva samples investigated. Statistical significance was observed between the detection of these bacteria and various oral conditions. However, count of SRB and the sulphide ion concentration in the tested samples did not have significant correlation with the oral conditions considered. Conclusion: The mere detection of SRB in saliva is of significance in the diagnoses of oral conditions. Enumeration of these bacteria and quantification of their metabolic end product in the same samples were not of diagnostic value."

Ion-selective electrode determination of sulphide produced by sulphate-reducing bacteria

1983 ◽  
Vol 20 (3) ◽  
pp. 119 ◽  
Author(s):  
I. K. Al-Hitti ◽  
G. J. Moody ◽  
J. D. R. Thomas ◽  
B. J. Birch ◽  
A. J. Frend ◽  
...  
Keyword(s):  
Ion Selective Electrode ◽  
Selective Electrode ◽  
Sulphate Reducing Bacteria ◽  
Reducing Bacteria

scholarly journals Determination of the Substrates for Sulphate-reducing Bacteria within Marine and Esturaine Sediments with Different Rates of Sulphate Reduction

Microbiology ◽  
1989 ◽  
Vol 135 (1) ◽  
pp. 175-187 ◽  
Author(s):  
R. J. PARKES ◽  
G. R. GIBSON ◽  
I. MUELLER-HARVEY ◽  
W. J. BUCKINGHAM ◽  
R. A. HERBERT
Keyword(s):  
Sulphate Reduction ◽  
Sulphate Reducing Bacteria ◽  
Reducing Bacteria

Determination of sulphide produced by Desulfovibrio species of sulphate-reducing bacteria

The Analyst ◽  
1983 ◽  
Vol 108 (1282) ◽  
pp. 43 ◽  
Author(s):  
I. K. Al-Hitti ◽  
G. J. Moody ◽  
J. D. R. Thomas
Keyword(s):  
Sulphate Reducing Bacteria ◽  
Reducing Bacteria ◽  
Desulfovibrio Species

scholarly journals Characterization of Oral Microbiota in Removable Dental Prosthesis Users: Influence of Arterial Hypertension

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Leila Maria Marchi-Alves ◽  
Dayana Freitas ◽  
Denise de Andrade ◽  
Simone de Godoy ◽  
Adrielle Naiara Toneti ◽  
...  
Keyword(s):  
Arterial Hypertension ◽  
Statistical Significance ◽  
Dental Prosthesis ◽  
Microbial Load ◽  
Oral Microbiota ◽  
Oral Infections ◽  
Dental Prostheses ◽  
The Social ◽  
Removable Dental Prosthesis

Introduction. Studies have described the possible relation between oral infections and atherosclerotic events.Objective. To characterize the oral microbiota of normotensive and hypertensive users of dental prostheses.Methods. The sample consisted of 41 complete dental prosthesis users, divided into groups: 21 participants with systemic arterial hypertension and 20 normotensive participants. The data collection included the characteristics of the sociodemographic variables and the determination of the microbial load in the saliva. For the descriptive analyses, Statistical Package for the Social Sciences was used. The description of the proportional differences between the groups was based on the application of Mann–Whitney’s statistical test. Statistical significance was set at 5% (p< 0.05).Results. The analysis of the oral microbiota showed the vast growth of aerobic microorganisms in all samples from both groups. The microbial load of streptococci and staphylococci was significantly higher among hypertensive participants.Candidayeasts were detected in the saliva culture of most samples. The hypertensive participants rank in the category of very high colonization index/high risk of infection related to this microorganism.Conclusions. The mouth of dental prosthesis users, especially when hypertensive, can constitute an important reservoir of pathogens, indicating an established inflammatory or infectious condition or risk for developing this condition.

scholarly journals Biocorrosive behavior of sulphate-reducing bacteria in kerr endodontic files: Determination of the corrosion

2020 ◽  
Vol 23 (2) ◽  
pp. 196
Author(s):  
FabianoLuiz Heggendorn ◽  
LuizAndré Lucas Teixeira Pinto ◽  
LucioSouza Gonçalves ◽  
Vivianede Oliveira Freitas Lione ◽  
WalterBarreiro Cravo Junior ◽  
...  
Keyword(s):  
Sulphate Reducing Bacteria ◽  
Reducing Bacteria

Detection of sulphate-reducing bacteria in human saliva

2013 ◽  
Vol 71 (6) ◽  
pp. 1458-1463 ◽  
Author(s):  
Fabiano Luiz Heggendorn ◽  
Lucio Souza Gonçalves ◽  
Eliane Pedra Dias ◽  
Arley Silva Junior ◽  
Mariana Machado Galvão ◽  
...  
Keyword(s):  
Human Saliva ◽  
Sulphate Reducing Bacteria ◽  
Reducing Bacteria

scholarly journals Initial Bacterial Adhesion and Biofilm Formation on Aligner Materials

Antibiotics ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 908
Author(s):  
Sibel Tektas ◽  
Thomas Thurnheer ◽  
Theodore Eliades ◽  
Thomas Attin ◽  
Lamprini Karygianni
Keyword(s):  
Bacterial Adhesion ◽  
Biofilm Formation ◽  
Statistical Significance ◽  
Sample Surface ◽  
Human Saliva ◽  
Bacterial Attachment ◽  
Columbia Blood Agar ◽  
Conventional Brackets ◽  
Aerobic And Anaerobic

The present study aims to assess the initial bacterial adhesion and biofilm formation on different aligner materials. A total of four different aligner materials, CA-medium (CAM), copolyester (COP), Duran (DUR), Erkodur (ERK), were tested. Stimulated human saliva was obtained from six healthy volunteers. Salivary bacteria were harvested by centrifugation, and 1 mL of the salivary suspension was injected onto each sample surface for 2 h and 3 days, respectively. The samples were then washed twice with 5 mL 0.9% NaCl solution, and non-adherent bacteria were removed. The adherent microorganisms were dislodged from the sample surfaces after ultrasonication for 4 min in 1 mL 0.9% NaCl on ice. After the incubation of the adherent salivary bacteria under both aerobic and anaerobic conditions on Columbia blood agar plates at 37 °C and 5% CO2 and in anaerobic jars overnight, several dilutions thereof were used for the determination of CFUs. This protocol was applied three times, obtaining an average of nine independent measurements for each material group. Overall, the differences between the tested aligner materials as well as between the materials and controls were not of statistical significance (p > 0.05). Regarding initial bacterial attachment and biofilm formation, the tested aligner materials are comparable to enamel and metal orthodontic brackets and can be therefore considered for clinical use. The four tested aligner materials CAM, COP, DUR, ERK showed no significant differences in initial microbial attachment and biofilm formation of aerobic and anaerobic species compared to enamel and conventional brackets.

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