Therapeutic effects of melatonin and quercetin on carbon tetrachloride-induced cardiac damage in rats /Sicanlarda karbon tetraklorur ile induklenen kardiyak hasar uzerine melatonin ve quercetin'in tedavi edici etkileri

2016 ◽  
Vol 23 (2) ◽  
pp. 171
Author(s):  
Hulya Elbe ◽  
Mukaddes Esrefoglu ◽  
Asli Taslidere ◽  
Elif Taslidere ◽  
Burhan Ates
Keyword(s):  
Carbon Tetrachloride ◽  
Therapeutic Effects ◽  
Cardiac Damage

Therapeutic Effects of Milk Thistle Extract against Renal Toxicity Induced By Diethylnitrosamine and Carbon Tetrachloride in Adult Rats

2017 ◽  
Vol 10 (1) ◽  
pp. 107-124
Author(s):  
Amal. Ahmed ◽  
Abeer Hassanin ◽  
Abeer Hassan ◽  
Saadia Ali ◽  
A. El-Anwar
Keyword(s):  
Carbon Tetrachloride ◽  
Renal Toxicity ◽  
Milk Thistle ◽  
Therapeutic Effects ◽  
Adult Rats

scholarly journals Curative Role of Dithiophenolato Titanium (IV)-Chitosan Nanocomposite Complex Against Carbon Tetrachloride-Induced Liver Injuries

2021 ◽  
Author(s):  
Nadia Z Shaban ◽  
Salah A Yehiaa ◽  
Doaa Awad ◽  
Shaban Y Shaban ◽  
Samar R Saleh
Keyword(s):  
Oxidative Stress ◽  
Carbon Tetrachloride ◽  
Lipid Profile ◽  
Hepg2 Cell ◽  
Chitosan Nanoparticles ◽  
Antibacterial Activities ◽  
Therapeutic Effects ◽  
Liver Histopathology ◽  
Liver Injuries ◽  
Kidney Functions

Abstract Background Titanium-based compounds have been incorporated as promising antineoplastic metals. In our previous studies, dithiophenolato titanium (IV) Complex "DBT" and its chitosan nanocomposite "DBT-CSNPs" were prepared and we showed that these compounds have antibacterial activities, cytotoxic, and have abilities to bind with DNA helixes. Therefore, in this study, we evaluated the LD50 values of dithiophenolato titanium (IV)-complex (DBT) and its high thermal stable chitosan nanoparticles (DBT-CSNPs). Then their therapeutic effects against liver injuries induced by carbon tetrachloride (CCl4) were assessed and compared with cisplatin treatment. Additionally, the anti-proliferative activity of DBT and DBT-CSNPs against human liver cancer (HepG2) cell lines through the analysis of the cell cycle was evaluated. Methods Nine groups of rats were prepared: normal, DBT, DBT-CSNPs, CSNPs, CCl4, CCl4-DBT, CCl4-DBT-CSNPs, CCl4-CSNPs and CCl4-cisplatin. Liver histopathology and the biochemical markers involving oxidative stress, apoptosis, liver and kidney functions, and lipid profile were determined. Results The results revealed that the treatment with DBT-CSNPs and DBT after CCl4 administration abolished liver damage since it reduced the apoptosis induced by CCl4 via the reduction of DNA fragmentation, Bax and caspase- 8 with an elevation of Bcl2 and Bcl2/Bax ratio. Also, these treatments caused nonsignificant changes in the markers of oxidative stress. Therefore, liver histopathology and functions, lipid profile, and kidney functions were improved. Cisplatin treatment reduced liver injury with a degree less than DBT-CSNPs and DBT, but it induced nephrotoxicity. Administration of DBT-CSNPs and DBT to healthy rats for 14 days has no adverse effect. Also, the results showed that DBT-CSNPs and DBT inhibited the proliferation of HepG2 cells by arresting cells in the G2/M phase and inducing cell death. Conclusion DBT-CSNPs and DBT have a therapeutic effect against CCl4-induced liver injuries via the reduction of apoptosis induced by CCl4. Moreover, both compounds have antineoplastic activities against the HepG2 cell line. In all cases, DBT-CSNPs have a greater effect due to their nanostructure. Therefore, both compounds can be used in the pharmacological fields, particularly DBT-CSNPs.

Protective and Therapeutic Effects of Curcuma xanthorrhiza on Hepatotoxin-induced Liver Damage

1995 ◽  
Vol 23 (03n04) ◽  
pp. 243-254 ◽  
Author(s):  
Song-Chow Li ◽  
Chun-Ching Lin ◽  
Yun-Ho Lin ◽  
S. Supriyatna ◽  
Chao-Wei Teng
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Damage ◽  
Intraperitoneal Administration ◽  
Therapeutic Effects ◽  
Acute Liver Damage ◽  
Liver Injuries ◽  
Acute Elevation ◽  
Serum Transaminases ◽  
Hepatoprotective Agent ◽  
Curcuma Xanthorrhiza

Curcuma xanthorrhiza Roxb. (Zingiberaceae family, commonly known as temu lawak or Javanese turmeric in Indonesia), which is found both wild and cultivated in Indonesia, has been traditionally used for medicinal purposes. C. xanthorrhiza is also used as a tonic in Indonesia. The aim of the present study is to clarify whether C. xanthorrhiza treatment may prevent acute liver damage induced by acetaminophen and carbon tetrachloride in mice. The results clearly indicated that extract of C. xanthorrhiza could reduce significantly the acute elevation of serum transaminases levels induced by the two kinds of hepatotoxins, and alleviated the degree of liver damage at 24 hours after the intraperitoneal administration of two hepatotoxins. It may be concluded that C. xanthorrhiza can protect the liver from various hepatotoxins, hence C. xanthorrhiza could be useful in the treatment of liver injuries and has promise as a kind of broad spectrum hepatoprotective agent.

Therapeutic Effects of CD133+ in the Carbon Tetrachloride (CCl4) Induced Chronic Liver Dysfunction in Rat Model

2015 ◽  
Vol 11 (4) ◽  
pp. 359-365
Author(s):  
Manouchehr Safari ◽  
Laya Ghahari ◽  
Katayoun Safari ◽  
Mahmoodreza Madadian ◽  
Mohammad Reza Aldaghi ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Rat Model ◽  
Liver Dysfunction ◽  
Chronic Liver ◽  
Therapeutic Effects

Preventive and therapeutic effects of oleuropein against carbon tetrachloride-induced liver damage in mice

2012 ◽  
Vol 65 (4) ◽  
pp. 451-464 ◽  
Author(s):  
Robert Domitrović ◽  
Hrvoje Jakovac ◽  
Vanja Vasiljev Marchesi ◽  
Ivana Šain ◽  
Željko Romić ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Damage ◽  
Therapeutic Effects

scholarly journals Therapeutic Effects and Associated Mechanisms by which “Fuyang Jiedu Huayu” Granules Treat Chronic Liver Failure in the Rat

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Qianling Ye ◽  
Hainan Jiang ◽  
Yanmei Lan ◽  
Minggang Wang ◽  
Dewen Mao
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Failure ◽  
Liver Tissue ◽  
Molecular Mechanisms ◽  
Chronic Liver ◽  
High Dose ◽  
Therapeutic Effects ◽  
Model Group ◽  
Chronic Liver Failure ◽  
Group Serum

Aim. Fuyang Jiedu Huayu (FYJDHY) granules are a combination of five traditional Chinese medicines with known therapeutic effects against chronic liver failure (CLF). The aim of the present study was to investigate the efficacy of FYJDHY to ameliorate the effects of carbon tetrachloride- (CCl4-) induced CLF in rats and to explore the possible molecular mechanisms underlying its therapeutic efficacy. Methods. A model of chronic liver failure was established by intraperitoneal injection of 50% carbon tetrachloride into SD rats for 8 weeks. After establishing the model, rats were treated with either low-dose (4.725 kg/d), medium-dose (9.45 kg/d), or high-dose (18.9 g/kg/d) FYJDHY for 2 weeks. After treatment, samples of liver tissue and blood were harvested from rats in each group. Serum ALT, AST, and TBIL levels and prothrombin time were measured using a biochemical analyzer. The expression of Gab1 (Grb2-associated binder 1), TPO (thrombopoietin), and its receptor c-Mpl were measured using quantitative real-time PCR (RT-PCR) and Western blot analysis, and assessment of histological improvement in liver tissue was by H&E-stained tissue sections. Results. Compared with the model group, serum ALT, AST, and TBIL levels and PT of rats in the intervention group were significantly reduced ( P < 0.05 ). In addition, FYJDHY alleviated pathological damage to liver tissue and increased the expression of Gab1, TPO, and its receptor c-Mpl in liver tissue, to levels statistically significant compared with the model group ( P < 0.05 ). Conclusions. The therapeutic effect of FYJDHY on CLF may be related to the promotion of angiogenesis and improvement in hemopoietic function in individuals suffering from CLF.

scholarly journals Hypoxia-challenged MSC-derived exosomes deliver miR-210 to attenuate post-infarction cardiac apoptosis

2020 ◽  
Author(s):  
Hao Cheng ◽  
Shufu Chang ◽  
Rende Xu ◽  
Lu Chen ◽  
Xiaoyue Song ◽  
...  
Keyword(s):  
Apoptotic Cell ◽  
Heart Function ◽  
Artery Bypass ◽  
Coronary Intervention ◽  
Luciferase Reporter ◽  
Therapeutic Effects ◽  
Cardiac Damage ◽  
Isolation And Identification

Abstract BackgroundMyocardial infarction (MI) is a major cause of death worldwide. Although percutaneous coronary intervention and coronary artery bypass grafting can prolong life, cardiac damage persists. In particular, cardiomyocytes have no regenerative capacity. Mesenchymal stem cells (MSCs) are attractive candidates for the treatment of MI. The manner by which MSCs exert a beneficial effect upon injured cells is a source of continued study.MethodsAfter the isolation and identification of exosomes from MSCs, the expression of miR-210 was determined by Microarray Chip. Subsequently, gain- and loss-function approaches were conducted to detect the role of exosomes and exosomal-miR-210 in cell proliferation and apoptosis of cardiomyocytes, as well as the MI in vivo. Dual-Luciferase Report Gene System was used to demonstrate the target gene of miR-210. ResultsWe tested the hypothesis that MSC-derived exosomes transfer specific miRNA to protect cardiomyocytes from apoptotic cell death. Interestingly, direct cardiac injection of MSC exosomes reduced infarct size and improved heart function after coronary ligation. In vitro, the MSC exosomes enhanced cardiomyocyte survival to hypoxia. Confirmation of exosome uptake in myocytes was confirmed. Dual luciferase reporter assay implicated miR-210 as a mediator of the therapeutic effect and AIFM3 as a downstream target. Treatment with miR-210 overexpressing MSC exosomes improved myocyte protection to both in vitro and in vivo stress. Furthermore, the endogenous and exogenous miR-210 had the same therapeutic effects. ConclusionThese results demonstrated that the beneficial effects offered by MSC-exosomes transplantation after MI are at least partially because of excreted exosome containing mainly miR-210.

scholarly journals Role of N-Acetylcysteine and Coenzyme Q10 in the Amelioration of Myocardial Energy Expenditure and Oxidative Stress, Induced by Carbon Tetrachloride Intoxication in Rats

Dose-Response ◽  
2018 ◽  
Vol 16 (3) ◽  
pp. 155932581879015 ◽  
Author(s):  
Nayira A. Abd Elbaky ◽  
Naglaa F. El-Orabi ◽  
Laila M. Fadda ◽  
Omar H. Abd-Elkader ◽  
Hanaa M. Ali
Keyword(s):  
Oxidative Stress ◽  
Carbon Tetrachloride ◽  
Coenzyme Q10 ◽  
Lipid Peroxides ◽  
Current Data ◽  
Cardiac Damage ◽  
Glucose Content ◽  
Myocardial Oxidative Stress ◽  
Acetyl Cysteine ◽  
Antioxidant Markers

This study is designed to evaluate the potential impact of N-acetyl cysteine (NAC) and coenzyme Q10 (CoQ10) each alone or in combination against carbon tetrachloride (CCl4)-induced cardiac damage in rats. Animals were treated with CCl4 in single intraperitoneal dose of 1 mL/Kg body weight; CCl4-intoxicated animals were pretreated with 20 mg/kg/d NAC or pretreated with 200 mg/kg/d CoQ10 or NAC and CoQ10 with the same previously mentioned doses. Carbon tetrachloride–intoxicated rats showed a significant elevation in nitric oxide and lipid peroxides and downregulation in reduced glutathione level and calcium adenosine triphosphatase. Cardiac glycolytic enzymes levels such as lactate dehydrogenase, phosphofructokinase, and hexokinase were declined coupled with a reduction in glucose content after CCl4 treatment. Moreover, myocardial hydroxyproline level was significantly increased after CCl4-treatment indicating accumulation of interstitial collagen. N-acetyl cysteine and/or CoQ10 effectively alleviated the disturbances in myocardial oxidative stress and antioxidant markers. These antioxidants effectively upregulated the reduction in cardiac energetic biomarkers due to CCl4 treatment. N-acetyl cysteine and/or CoQ10 significantly decreased hydroxyproline level compared to that of CCl4-treated rats. The current data showed that the aforementioned antioxidants have a remarkable cardioprotective effect, suggesting that they may be useful as prophylactic agents against the detrimental effects of cardiotoxins.

Sign in / Sign up

Export Citation Format

Share Document