scholarly journals Characterization of immunogenic proteins of Cysticercus tenuicollis of goats

2013 ◽  
Vol 6 (5) ◽  
pp. 267
Author(s):  
A Goswami ◽  
M Das ◽  
R Laha
Keyword(s):  
Cysticercus Tenuicollis ◽  
Immunogenic Proteins

Characterization of the main immunogenic proteins in Brucella infection for their application in diagnosis of brucellosis

2020 ◽  
Vol 70 ◽  
pp. 101462 ◽  
Author(s):  
Mingxing Tian ◽  
Meiying Song ◽  
Yi Yin ◽  
Zhengmin Lian ◽  
Zichen Li ◽  
...  
Keyword(s):  
Brucella Infection ◽  
Immunogenic Proteins

scholarly journals Genetic characterization of three mitochondrial gene sequences of goat/sheep-derived coenurus cerebralis and cysticercus tenuicollis isolates in Inner Mongolia, China

Parasite ◽  
2018 ◽  
Vol 25 ◽  
pp. 1 ◽  
Author(s):  
Yichi Zhang ◽  
Wei Zhao ◽  
Di Yang ◽  
Yuan Tian ◽  
Weizhe Zhang ◽  
...  
Keyword(s):  
Inner Mongolia ◽  
Genetic Characterization ◽  
Mitochondrial Gene ◽  
Northern China ◽  
Nucleotide Sequences ◽  
Taenia Hydatigena ◽  
Cysticercus Tenuicollis ◽  
Coenurus Cerebralis ◽  
Epidemiological Significance

Taenia multiceps and Taenia hydatigena are widely distributed tapeworms of canids. Due to a lack of genetic information on these two parasites in China, in this study we analyzed six coenurus cerebralis and two cysticercus tenuicollis cysts from goats or sheep in Inner Mongolia, northern China by amplifying three mitochondrial genes (cox1, nad4, and cytb). Two haplotypes were obtained at each locus for either of the two Taenia cestode species, with ten nucleotide sequences being novel. The degrees of genetic variations were 1.18%, 0.61% and 0.52% for coenurus cerebralis, and 0.24%, 0.46% and 0.35% for cysticercus tenuicollis at the cox1, nad4 and cytb loci, respectively. This is the first molecular description of animal-derived metacestodes of T. multiceps and T. hydatigena in Inner Mongolia, China. Novel nucleotide sequences might reflect endemic genetic characterization of the two cestodes. The present data are useful to explore the biological and epidemiological significance of intra-specific variations within both Taenia cestodes.

scholarly journals Prevalence and Molecular Characterization of Cysticercus tenuicollis Isolated from Some Intermediate Host in Kurdistan-Iraq

2018 ◽  
pp. 177-182
Author(s):  
Abdullah Ahmed Hama ◽  
Rostam Hama Zorab ◽  
Fatima Mohammed Ali ◽  
Awaz M. Salih ◽  
Amer Abdullah Hassan
Keyword(s):  
Hydatid Cyst ◽  
Molecular Characterization ◽  
Dna Sequences ◽  
Prevalence Rate ◽  
Accession Number ◽  
Taenia Hydatigena ◽  
Sheep And Goats ◽  
Cysticercus Tenuicollis ◽  
Nucleotide Sequence Alignment

Cysticercus tenuicollis (C. tenuicollis) is the larval stage infection of Taenia hydatigena, a common tapeworm of dogs and other Canidae, which has a wide range of intermediate hosts including sheep, goat, cattle, deer, camel, horse, human and other wild ruminants, the disease spreads through a contaminated water, soil and food with feces of infected dogs or other carnivores, T. hydatigena lives in the intestinal of the definitive host (carnivores) and excretes a huge number of eggs with feces daily. The present study conducted to determine the prevalence rate and molecular characterization of C. tenuicollis among sheep and goats in Sulaimani province. A total of 14088 slaughtered animals were inspected postmortem from the new Sulaimani abattoir comprise, which involves 13395 sheep and 693 goats. The selected cysts were preserved in 70% Ethanol for DNA extraction and molecular study,  The mt-CO1 gene was amplified with a conventional polymerase chain reaction (PCR), the PCR product purified and DNA sequencing for reverse and forward strands was determined by a genetic analyzer, the obtained sequences aligned with the DNA sequences of T. hydatigena in Iran, Turkey and Palestine, which deposited in GenBank under the following accession number (JQ710588), (JN827307) and ( KM032284) respectively. The prevalence rate of C. tenuicollis was 2.63% in sheep and 2.58% in goats. This result shows no significant differences of C. tenuicollis between sheep and goats (p>0.05). The nucleotide sequence alignment of cytochrome oxidase subunit 1 (CO 1) gene revealed that the amplified DNA fragment is belong to Taenia hydatigena and Echinococcus granulosus and the nucleotide sequences of T. hydatigena deposited in GenBank under accession number (MH638348). This finding concludes that the amplification of mt-CO1 gene cannot be depended on discriminate hydatid cyst and C. tenuicollis while the partial DNA sequences of mt-CO1 gene are significantly valuable to differentiate C. tenuicollis from hydatid cyst, which is completely different in the pathology and control.

scholarly journals In silico secretome characterization of clinical Mycobacterium abscessus isolates provides insights into antigenic differences

2020 ◽  
Author(s):  
Fernanda Cornejo-Granados ◽  
Thomas A. Kohl ◽  
Flor Vásquez Sotomayor ◽  
Sönke Andres ◽  
Rogelio Hernández-Pando ◽  
...  
Keyword(s):  
Drug Targets ◽  
Reference Strain ◽  
Mycobacterium Abscessus ◽  
Bioinformatics Pipeline ◽  
Diagnostic Assays ◽  
Essential Proteins ◽  
Immunogenic Proteins ◽  
Drugbank Database

AbstractMycobacterium abscessus (MAB) is a widely disseminated pathogenic non-tuberculous mycobacterium (NTM). Like with M. tuberculosis complex (MTBC), excreted / secreted (ES) proteins play an essential role for its virulence and survival inside the host. ES proteins contain highly immunogenic proteins, which are of interest for novel diagnostic assays and vaccines. Here, we used a robust bioinformatics pipeline to predict the secretome of the M. abscessus ATCC 19977 reference strain and fifteen clinical isolates belonging to all three MAB subspecies, M. abscessus subsp. abscessus, M. abscessus subsp. bolletii, and M. abscessus subsp. massiliense. We found that ~18% of the proteins encoded in the MAB genomes were predicted as secreted and that the three MAB subspecies shared > 85 % of the predicted secretomes. MAB isolates with a rough (R) colony morphotype showed larger predicted secretomes than isolates with a smooth (S) morphotype. Additionally, proteins exclusive to the secretomes of MAB R variants had higher antigenic densities than those exclusive to S variants, independently of the subspecies. For all investigated isolates, ES proteins had a significantly higher antigenic density than non-ES proteins. We identified 337 MAB ES proteins with homologues in previously investigated M. tuberculosis secretomes. Among these, 222 have previous experimental support of secretion, and some proteins showed homology with protein drug targets reported in the DrugBank database. The predicted MAB secretomes showed a higher abundance of proteins related to quorum-sensing and Mce domains as compared to MTBC indicating the importance of these pathways for MAB pathogenicity and virulence. Comparison of the predicted secretome of M. abscessus ATCC 19977 with the list of essential genes revealed that 99 secreted proteins corresponded to essential proteins required for in vitro growth. All predicted secretomes were deposited in the Secret-AAR web-server (http://microbiomics.ibt.unam.mx/tools/aar/index.php).

scholarly journals Identification and Immunological Characterization of Somatic Proteins from Adults of Toxocara cati by Proteomics Technique

2021 ◽  
Author(s):  
Nooshinmehr SOLEYMANI ◽  
Ruth Birner GRUNBERGER ◽  
Khalil ABNOUS ◽  
Hassan BORJI ◽  
Faezeh VAHDATI
Keyword(s):  
Drug Targets ◽  
Immunoblot Analysis ◽  
Toxocara Cati ◽  
Additional Information ◽  
Immunogenic Proteins ◽  
Etiological Agents ◽  
Paratenic Hosts ◽  
Medical University ◽  
Somatic Antigens

Background: Toxocara cati is considered as one of the main etiological agents of toxocariasis with global and regional importance. As there is no information on proteomics of T. cati, herein, we reported the results obtained by proteomic analysis of somatic proteins extract, using a mass spectrometry (LC–MS/MS) approach. Methods: Somatic extract fractions were separated by two-dimensional SDSPAGE and were electro blotted on to PVDF membranes for immunoblot analysis, then collected the immunogenic spots which response of antibodies of the paratenic hosts (mice) to the antigens ( Mashhad, 2017), and analyzed by LC–MS/MS. The LC-MS/MS data were analyzed by Mascot database, Taxonomy Toxocara, and common contaminants, in Omics Center, Biotechnology Medical University of Graz (Austria, 2018). Result: The protein spots were isolated between 15–140 kDa ranges using 3–10 non-linear IPG strips and Brilliant Blue Coomassie. Ten proteins were characterized as immunogenic proteins, seven of them were identified and three of them were unknown proteins. Conclusion: This study provided additional information about the somatic antigens of T. cati, which can lead to the development of new strategies for novel immuno-modulators, drug targets, subunit vaccines and immunodiagnostic kits for toxocariasis.

Identification and Characterization of Novel Immunogenic Proteins ofStreptococcus suisSerotype 2

2008 ◽  
Vol 7 (9) ◽  
pp. 4132-4142 ◽  
Author(s):  
Hongran Geng ◽  
Li Zhu ◽  
Yuan Yuan ◽  
Wei Zhang ◽  
Wenjun Li ◽  
...  
Keyword(s):  
Immunogenic Proteins ◽  
Identification And Characterization

Molecular characterization and haplotypes of sheep and goat isolates of Cysticercus tenuicollis in Turkey

Parasitology ◽  
2019 ◽  
Vol 146 (8) ◽  
pp. 1047-1054 ◽  
Author(s):  
Seyma Gunyakti Kilinc ◽  
Harun Kaya Kesik ◽  
Sami Simsek
Keyword(s):  
Genetic Diversity ◽  
Nadh Dehydrogenase ◽  
Genetic Characterization ◽  
Mature Stage ◽  
Sequence Analyses ◽  
Taenia Hydatigena ◽  
Cysticercus Tenuicollis ◽  
Pcr Products ◽  
Common Parasite

AbstractTaenia hydatigena, is a common parasite, lived mostly in dogs and wild carnivores in its mature stage, and the larvae, Cysticercus tenuicollis, is found on ruminants and pigs. The aim of the current study was to determine the genetic diversity in 20 isolates of the sheep and goats. After the isolation of total genomic DNA from C. tenuicollis isolates, genetic characterization of the mitochondrial NADH dehydrogenase subunit 1 gene region was amplified using specific JB11–JB12 primers in PCR and the PCR products were sequenced and haplotype and genetic diversity analyses were utilized. As a result, multiple nucleotide changes were determined in the sequence analyses of the isolates leading to detection of 16 and 15 different haplotypes in sheep and goat samples, respectively. These findings are important in terms of showing the diversity of nucleotide variation in C. tenuicollis in Turkey.

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