scholarly journals Development and Validation of RP-HPLC Method for Simultaneous Qualitative and Quantitative Estimation of Curcumin and Quercetin in Bulk Mixture

2022 ◽  
Vol 56 (1) ◽  
pp. 247-254
Author(s):  
Nayana Patil ◽  
Hitendra Mahajan
Keyword(s):  
Quantitative Estimation ◽  
Hplc Method ◽  
Qualitative And Quantitative ◽  
Rp Hplc ◽  
Development And Validation

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF LERCANIDIPINE IN BIOLOGICAL MATRICES

INDIAN DRUGS ◽  
2012 ◽  
Vol 49 (07) ◽  
pp. 49-53
Author(s):  
A Singla ◽  
◽  
Y. Paul ◽  
B. Singh
Keyword(s):  
Limit Of Detection ◽  
Quantitative Estimation ◽  
Reversed Phase ◽  
Hplc Method ◽  
Biological Matrices ◽  
Limit Of Quantification ◽  
Rp Hplc ◽  
Uv Visible ◽  
Development And Validation ◽  
Isocratic Mode

A simple, precise, accurate, reproducible and robust analytical method was developed using reversed phase HPLC for quantitative estimation of lercanidipine in biological matrices. The method was validated as per the ICH and FDA guidelines. Analysis of the drug was performed in an isocratic mode employing methanol and acetonitrile (74:26) as the mobile phase on a C18 column. UV-Visible detector at 219 nm was found to be suitable for drug estimation. Linearity was observed in the range of 5 and 500 ng/mL (p<0.001). Recovery was found to range between 96.26% and 100.67%. The % RSD values for method precision and system precision were found to be 0.437 and 1.58, respectively. Limit of detection (LOD) and limit of quantification (LOQ) were observed to be 0.20 ng/mL and 0.68 ng/mL, respectively.

scholarly journals Development and validation of RP-HPLC method for the quantitative estimation of αs1-genetic variants in goat milk

2014 ◽  
Vol 156 ◽  
pp. 165-169 ◽  
Author(s):  
Maria Montalbano ◽  
Lina Tortorici ◽  
Salvatore Mastrangelo ◽  
Maria Teresa Sardina ◽  
Baldassare Portolano
Keyword(s):  
Genetic Variants ◽  
Quantitative Estimation ◽  
Goat Milk ◽  
Hplc Method ◽  
Rp Hplc ◽  
Development And Validation

DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR QUANTITATIVE ESTIMATION OF NUCIFERINE FROM LOTUS LEAVES

INDIAN DRUGS ◽  
2016 ◽  
Vol 53 (12) ◽  
pp. 37-41
Author(s):  
N. A. Patel ◽  
◽  
A. P. Jadhav
Keyword(s):  
Mobile Phase ◽  
Orthophosphoric Acid ◽  
Quantitative Estimation ◽  
Hplc Method ◽  
Quality And Safety ◽  
Herbal Drugs ◽  
Perennial Plant ◽  
Rp Hplc ◽  
Lotus Leaves ◽  
Development And Validation

Nelumbo nucifera Gaertn (Family: Nelumbonaceae), commonly known as lotus, is an aquatic perennial plant. The lotus leaves are used to treat fever, hematemesis, hyperlipidaemia and obesity. The leaves are rich in alkaloids such as nuciferine, roemerine, pronuciferine and N-nornuciferine. The present study describes simple, accurate, precise, robust RP-HPLC method to detect and quantitate nuciferine as a marker from lotus leaves. The marker was resolved using Hemochrom Intsil C-18 column, with mobile phase methanol:10mm phosphate buffer (85:15), pH adjusted to 4 (using orthophosphoric acid) at a flow rate of 1 mL/min. Retention time of nuciferine was 5.60±0.2 minutes at 272 nm. Linear responses were obtained in concentration range of 1 - 9 ppm for nuciferine with correlation coefficient (r2) of 0.9996. The developed method was applied for quantitation of marker from lotus leaves. This method can be used to evaluate nuciferine containing extracts, thus conforming to the need of ensuring quality and safety of herbal drugs.

scholarly journals New Cost-Effective RP-HPLC Method Development and Validation for Quantitative Estimation of Ivacaftor in Pharmaceutical Formulation

2021 ◽  
Vol 71 (1) ◽  
Author(s):  
Roshani Singh ◽  
Omray L K ◽  
Pushpendra Soni
Keyword(s):  
Method Development ◽  
Quantitative Estimation ◽  
Cost Effective ◽  
Hplc Method ◽  
Pharmaceutical Formulation ◽  
Experimental Result ◽  
Rp Hplc ◽  
Method Development And Validation ◽  
Hplc Method Development ◽  
Development And Validation

In this article “new cost-effective RP-HPLC method development and validation for quantitative estimation of ivacaftor in the pharmaceutical formulation” developed. This study includes RP-HPLC Spectrophotometric method development, such as economical and simple HPLC method was optimized during development and validated accordingly in tablets of ivacaftor. The developed method may utilize for the analysis of ivacaftor at the laboratory level. The result shows that developed methods are cost-effective, rapid (Short retention time), simple, accurate (the value and %RSD between 2-5), precise, and can be used for the intended purpose on the tablet dosage form. The present proposed method is capable of better separation of analyte and qualifies on the point of analytical validation such as linearity, specificity, accuracy, precision, robustness, LOD, and LOQ on a marketed formulation. The simplicity, rapidity, and reproducibility of the developed method qualify the objective of the research. Results of analysis of the ivacaftor tablet formulations are arranged in the experimental, result, and discussion section. The portion of ivacaftor found in terms of quantity was between 98-102% and also within USP 29 chapter (541) acceptance criteria.

scholarly journals DEVELOPMENT AND VALIDATION OF A RP-HPLC METHOD FOR THE SIMULTANEOUS DETERMINATION OF CURCUMIN, PIPERINE AND CAMPHOR IN AN AYURVEDIC FORMULATION

2018 ◽  
Vol 10 (4) ◽  
pp. 115
Author(s):  
Sultana Shaikh ◽  
Vandana Jain
Keyword(s):  
High Performance ◽  
Quantitative Estimation ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Column Temperature ◽  
Rp Hplc ◽  
Relative Standard ◽  
Precision Study ◽  
Development And Validation ◽  
Liquid Chromatographic

Objective: To develop a novel, accurate, precise and linear reverse phase high performance liquid chromatographic (RP-HPLC) method for simultaneous qualitative and quantitative estimation of curcumin, piperine and camphor in an ayurvedic formulation and validate as per international conference on harmonization (ICH) guidelines.Methods: In the present work, good chromatographic separation was achieved isocratically using a shim-pack HPLC C18 column (4.6 x 250 mm, 5μm) and mobile phase consisting of 0.02 M potassium dihydrogen orthophosphate buffer (pH adjusted to 3.5 with orthophosphoric acid) and acetonitrile in the ratio 40:60, at flow rate of 1 ml/min and column temperature maintained at 35 °C. The effluents obtained were monitored at 255 nm with UV-visible detector.Results: The retention time of curcumin, piperine and camphor was found to be 6.57 min, 7.32 min and 8.57 min respectively. Linearity of curcumin and camphor were found in the range of 4-8 ppm and that of piperine was found to be 5-9 ppm. The correlation coefficient for curcumin, piperine and camphor were 0.998, 0.99 and 0.994 respectively. The high recovery values (98 %-102 %) indicate a satisfactory accuracy. The low percent relative standard deviation (% RSD) values in the precision study reveals that the method is precise.Conclusion: The developed method is novel, simple, precise, rapid, accurate and reproducible for simultaneous quantitative estimation of curcumin, piperine and camphor in an ayurvedic formulation. Hence the developed method can be used for quantitative analysis and quality control of extracts and commercial samples of other species containing these three markers.

scholarly journals DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR PHENYTOIN SODIUM AND PHENOBARBITONE IN BULK AND PHARMACEUTICAL DOSAGE FORM

2017 ◽  
Vol 9 (10) ◽  
pp. 224
Author(s):  
Radhika Shah ◽  
Ragin Shah
Keyword(s):  
Dosage Form ◽  
Quantitative Estimation ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Pharmaceutical Dosage Form ◽  
Rp Hplc ◽  
Phenytoin Sodium ◽  
Pharmaceutical Industries ◽  
Development And Validation ◽  
Tablet Dosage

Objective: To develop an accurate, simple, rapid, precise, and linear RP-HPLC method for the simultaneous estimation of phenytoin sodium and phenobarbitone in tablet dosage form and validated as per ICH guidelines.Methods: The method used was a reverse phase HPLC (RP-HPLC) method using Hypersil BDS C18, (250×4.6 mm, 5 μm) column, mobile phase comprising of methanol: phosphate buffer (pH 5.0) (50:50), flow rate of 1.0 ml/min and a detection wavelength of 215 nm using a UV detector.Results: The retention time for phenytoin sodium and phenobarbitone was found to be 3.97 min and 6.90 min, respectively. The linearity of developed method was achieved in the range of 10-30 μg/ml for phenytoin sodium and 3-9 μg/ml for phenobarbitone. The detection (LOD) and quantitation (LOQ) limits were 1.44 and 4.36 µg/ml for phenytoin sodium and 0.40 and 1.35 µg/ml for phenobarbitone respectively.Conclusion: A simple, accurate, precise, linear and rapid RP-HPLC method was developed for simultaneous quantitative estimation of phenytoin sodium and phenobarbitone in bulk and pharmaceutical formulation. The method was validated as per ICH guidelines. Hence, the method holds good for the routine analysis of phenytoin sodium and phenobarbitone in various pharmaceutical industries as well as in academics.

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