The Effect Of Immunizing Swiss White Mice With Snail Soluble Proteins And Challenging Them With Schistosoma Mansoni

doi:10.5580/2b3f

Soluble proteins from Schistosoma mansoni and Japonicum: A comparative biochemical and immunological analysis

Comparative Biochemistry and Physiology Part B Comparative Biochemistry ◽

10.1016/0305-0491(89)90388-x ◽

1989 ◽

Vol 93 (3) ◽

pp. 635-642

Author(s):

M.K. Abbas ◽

L.A. Foster ◽

G.D. Cain

Keyword(s):

Schistosoma Mansoni ◽

Soluble Proteins ◽

Immunological Analysis

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Cellular responses against Schistosoma mansoni in immunized Balb/c mice with soluble proteins from intermediate host, Biomphalaria pfeifferi

Tanzania Journal of Health Research ◽

10.4314/thrb.v14i4.7 ◽

2012 ◽

Vol 14 (4) ◽

Author(s):

Kennedy M. Kuria ◽

Rebecca Waihenya ◽

Hellen Kutima ◽

Peninnah Njoki ◽

Dorcas S. Yole

Keyword(s):

Schistosoma Mansoni ◽

Digestive Gland ◽

Cellular Responses ◽

Chemotherapeutic Agents ◽

Soluble Proteins ◽

Cell Mediated Immunity ◽

Biomphalaria Pfeifferi ◽

Assay Time ◽

Ifn Γ ◽

Considerable Morbidity

Scores of millions of people around the world are infected by Schistosoma mansoni causing considerable morbidity, mortality and loss of productivity. Safe chemotherapeutic agents have been used though there are challenges of re-infection due to resistance. Both epidemiological and experimental data suggest that acquired cell mediated immunity play significant roles in regulating the intensity of S. mansoni infection as well as its patho-physiologic sequelae. Improved control of this trematode parasite may be obtained with immunization to enhance the resistance of individuals to risk of infection. This study investigated the cellular responses of mice immunized with soluble proteins from foot and digestive gland of the vector snail and challenged with S. mansoni. The proteins were used to immunize the experimental groups then challenged with the S. mansoni. The experimental groups were FT (immunized with foot protein) and DG (immunized with digestive gland). The parameters, which were analyzed to demonstrate protection, included; the worm counts and cellular (IFN-γ, IL-5 cytokines) responses. It was observed that, the experimental groups showed significant protection in terms of worm reduction and immune responses. The group vaccinated with foot protein showed higher protection (87.5%) as compared to the group vaccinated with the digestive gland (50%) in terms of worm reduction. Cytokines (IFN-γ and IL-5) production was present in different levels during the assay time points which showed an aspect of protection. The Foot protein of the vector showed more immunizing power than the digestive gland. Research towards utilizing the two proteins as feasible vaccine candidates is encouraged

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Ultrastructural analysis of “Sensory” surface nerve endings and “putative” neurotransmitter sites in Schistosoma mansoni Miracidia

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100156791 ◽

1989 ◽

Vol 47 ◽

pp. 962-963

Author(s):

Betty Ruth Jones ◽

Steve Chi-Tang Pan

Keyword(s):

Nervous System ◽

Life Cycle ◽

Schistosoma Mansoni ◽

Snail Host ◽

Complex Life Cycle ◽

Rational Approach ◽

Effective Control ◽

The Social ◽

Social And Economic Development ◽

Basic Biology

INTRODUCTION: Schistosomiasis has been described as “one of the most devastating diseases of mankind, second only to malaria in its deleterious effects on the social and economic development of populations in many warm areas of the world.” The disease is worldwide and is probably spreading faster and becoming more intense than the overall research efforts designed to provide the basis for countering it. Moreover, there are indications that the development of water resources and the demands for increasing cultivation and food in developing countries may prevent adequate control of the disease and thus the number of infections are increasing.Our knowledge of the basic biology of the parasites causing the disease is far from adequate. Such knowledge is essential if we are to develop a rational approach to the effective control of human schistosomiasis. The miracidium is the first infective stage in the complex life cycle of schistosomes. The future of the entire life cycle depends on the capacity and ability of this organism to locate and enter a suitable snail host for further development, Little is known about the nervous system of the miracidium of Schistosoma mansoni and of other trematodes. Studies indicate that miracidia contain a well developed and complex nervous system that may aid the larvae in locating and entering a susceptible snail host (Wilson, 1970; Brooker, 1972; Chernin, 1974; Pan, 1980; Mehlhorn, 1988; and Jones, 1987-1988).

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