scholarly journals Chlorophyll formation in callus tissues, cultured in vitro, of explants isolated from various root parts of four carrot varieties

2015 ◽  
Vol 37 (2) ◽  
pp. 207-216
Author(s):  
T. Ruebenbauer ◽  
W. Miszke ◽  
B. Skucińska
Keyword(s):  
Callus Tissues ◽  
Chlorophyll Formation

scholarly journals Isolation of furanocoumarins from Pastinaca sativa L. callus culture

2014 ◽  
Vol 69 (3) ◽  
pp. 193-195 ◽  
Author(s):  
Halina Ekiert ◽  
Wanda Kisiel
Keyword(s):  
Secondary Metabolites ◽  
Callus Culture ◽  
Spectral Methods ◽  
Solid Medium ◽  
Intact Plant ◽  
Pastinaca Sativa ◽  
First Report ◽  
Callus Tissues ◽  
First Time

Four furanocoumarins: bergapten, xanthotoxin, isopimpinellin (linear furanocoumarins) and sphondin (angular furanocoumarin) were isolated for the first time from callus tissues of <em>Pastinaca sativa</em> L.(<em>Apiaceae</em>) cultured in vitro on solid medium. The compounds were identified using spectral methods. They are well-known secondary metabolites of the intact plant. This is the first report on the isolation of sphondin from in vitro plant cultures.

scholarly journals Qualitative and Quantitative Evaluation of Active Constituents in Callus of Lavandula angustifolia plant in Vitro

2020 ◽  
Vol 17 (2(SI)) ◽  
pp. 0591
Author(s):  
Zainab Salman et al.
Keyword(s):  
Leaf Explants ◽  
Volatile Oil ◽  
Gas Chromatography Mass Spectrometry ◽  
Naphthalene Acetic Acid ◽  
Fresh Weight ◽  
Lavandula Angustifolia ◽  
Qualitative And Quantitative ◽  
Main Components ◽  
Callus Tissues

This study was conducted to describe a protocol for the callus establishing culture of Lavandula angustifolia plant and estimating their content of volatile oil. The quantity of volatile oil callus tissues was compared with that of leaves production. Callus was induced from leaf explants on Murashige and Skoog medium (MS) supplemented with Naphthalene acetic acid (NAA) and Benzyl adenine (BA) in different concentrations. Maximum callus fresh weight was obtained in the combination of 10 mg/L BA and 3 mg/L NAA which reached 18 g after four weeks. The results of this work showed that the  quantity of volatile oil from the highest fresh weight callus was 6 ml compared with quantity of 18g of leaves which gave 0.5 ml. Volatile oil of leaf and callus extracts were analyzed using gas chromatography mass spectrometry method (GC-MS) which showed linoleic acid (56.61%) and oleic acid (57.93%) as main components.

Analysis of ancient DNA from in vitro grown tissues of 1600-year-old seeds revealed the species as Anagyris foetida

2012 ◽  
Vol 22 (4) ◽  
pp. 279-286 ◽  
Author(s):  
Murat Özgen ◽  
Aslı Özdilek ◽  
Melahat A. Birsin ◽  
Sertaç Önde ◽  
Derya Şahin ◽  
...  
Keyword(s):  
Nuclear Dna ◽  
Adventitious Shoots ◽  
Its Region ◽  
Naphthaleneacetic Acid ◽  
Archaeological Excavation ◽  
Relict Species ◽  
Western Turkey ◽  
Callus Tissues ◽  
Seed Characteristics

AbstractSeven ancient seeds, about 1600 years old, were found during an archaeological excavation in Asar Island which is located in south-western Turkey. These seeds were subjected to germination, in vitro callus induction and molecular characterization experiments to test the viability and plant origin of the seeds. Six of the seven seeds had viable seed components (such as cotyledons) and produced callus tissue in Murashige and Skoog medium supplemented with 2 mg l− 1 6-benzylamino purine (BAP), 0.2 mg l− 1 1-naphthaleneacetic acid (NAA), 20 g l− 1 sucrose, 2 mg l− 1 glycine and 7 g l− 1 agar, but the calli from these seeds failed to yield adventitious shoots. DNA samples from callus tissues produced by ancient seeds in vitro were of good quality. The internal transcribed spacer (ITS) region in nuclear DNA (nDNA) of ancient seeds was amplified successfully. The sequences from amplified ITS DNA products of six ancient seeds indicated that their ITS sequences matched those of Anagyris foetida after subjecting them to BLAST searches in international sequence databases (NCBI). A. foetida is a relict species endemic to the Mediterranean region and used as a herbal medicine. We believe that seed characteristics such as the very hard, extremely smooth and shiny testa, toxic anagyrine alkaloid content and their storage in a pot further improved the longevity of these ancient seeds.

scholarly journals Dr. Ottó Orsós, the forgotten Hungarian pioneer in plant tissue culture

2005 ◽  
Vol 11 (1) ◽  
Author(s):  
M. G. Fári
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Cell Biology ◽  
In Vitro Morphogenesis ◽  
Regenerated Plants ◽  
Universal Biology ◽  
History Of ◽  
Callus Tissues

The knowledge of tissue culture deserves attention in respect of understanding the development of universal biology. This study intends to contribute to the past of the plant tissue culture by such data of the history of science which have been unprocessed so far. It seems that the life-work of the Hungarian biologist, Dr. Ottó Orsós is a missing and essential link between those early plant hormone researchers and the representatives of the pioneers of tissue culture schools who have contributed substantially to the development of the modern in vitro plant morphogenesis and plant cell biology. Orsós cultured kohlrabi tuber cubes on White culture medium in a sterile manner. This way, he could efficiently direct the in vitro morphogenesis of the kohlrabi, the regeneration of its shoot and root, and the formation and steps to subculture of pure callus tissues in 1938. He supported the correctness of its statements by means of detailed anatomical examinations. Orsós successfully rooted and aclimatized complete regenerated plants. We may as well call the above system — in remembrance of the creators of the original concept — "Haberlandt-Orsós model". Between the publishing of his main paper in 1938 and 2003, a period of 65 years has lapsed. On the occasion of this anniversary, we bow before this forgotten pioneer.

scholarly journals Morphogenic processes in callus tissue cultures and de novo regeneration of plants in Actinidia chinensis Planch

2014 ◽  
Vol 67 (3-4) ◽  
pp. 217-222 ◽  
Author(s):  
Adela Ludvová ◽  
Mária G. Ostrolucká
Keyword(s):  
De Novo ◽  
Leaf Explants ◽  
Structural Heterogeneity ◽  
Callus Cultures ◽  
Culture Conditions ◽  
Actinidia Chinensis ◽  
Indirect Organogenesis ◽  
Vascular Elements ◽  
Callus Tissues

Our experiments have confirmed the considerable disposition of leaf explants of <em>Actinidia chinensis</em> Planch. for induction and intensive proliferation of callus cultures, as well as, a possibility to regulate morhogenesis in in vitro conditions. Under specific culture conditions the morphogenic potential of callus cells of <em>Actinidia chinensis</em> was manifested both in organogenesis and somatic embryogenesis. Organogenesis was represented by induction of adventitious buds and regeneration shoots on the modified MS culture medium (Murashige and Skoog 1962) with BAP in combination with GA<sub>3</sub> (each 1.0 mg. l<sup>-1</sup>). Rooting of shoots was successful on modified MS medium containing IBA (0.5-1.0 mg. l<sup>-1</sup>). Histological studies of callus tissues revealed their structural heterogeneity. Morphogenic processes in the callus were characterized by the appearance of meristematic zones and vascular elements. The formation of apical meristem, leaf primordia and finally shoot development proved de novo regeneration in callus culture. The obtained results demonstrate a possibility of plant regeneration through indirect organogenesis, which can be used for propagation of<em> Actinidia chinensis</em> Planch.

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