scholarly journals The effect of exogenous acetylcholine and other cholinergic agents on photoperiodic flower induction of Pharbitis nil

2014 ◽  
Vol 66 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Hanna Łukasiewicz-Rutkowska ◽  
Andrzej Tretyn ◽  
Mariusz Cymerski ◽  
Jan Kopcewicz

Exogenous acetylcholine (ACh), acetylcholinesterase (AChE) inhibitors, as well as agonists and antagonists of ACh receptors applied on the cotyledons of 5-day-old seedlings of <em>Pharbitis nil</em> during a 16-hour long inductive night or during a 12-hour long subinductive night, do not essentially influence the flower bud formation. Also the application of above mentioned substances to the seedlings growing under the conditions of 72 hours of darkness, 24 hours of light and then 24 hours of darkness does not influence in an essential way flowering of <em>P. nil</em>. On the other hand, applying these substances on the cotyledons of <em>P. nil</em> during 24-hour-long inductive night, preceded by 72 hours of darkness and then 24 hours of light of lowered intensity finished by 15-minute-long impulse of far red light which inhibit flowering, caused the reversion of the effect of far red light irradiation and stimulated the flowering. The obtained results suggest that endogenous system ACh/AChE could participate in the mechanism of a phytochrome controlled flowering of short day plants.

2014 ◽  
Vol 67 (1) ◽  
pp. 59-63 ◽  
Author(s):  
Halina Kulikowska-Gulewska ◽  
Elżbieta Galoch ◽  
Jan Kopcewicz

The endogenous content of ABA in the cotyledons of <em>Pharbitis nil</em> is high during the light phase before an inductive 16-h-long dark period. During the night, however, at the beginning, the level of ABA is relatively low with the tendency to increase during the second half of an inductive dark period. The dual effect of exogenous ABA on the <em>Pharbitis nil</em> flowering has been observed. ABA applied to the cotyledons on subthreshold photoperiod (12-h-long night) stimulates flower bud formation. On the other hand, however, ABA applied during an inductive (16-h-long) dark period, as well as applied to the medium of cultured plantlets, inhibits flowering. Thus, the flowering effect of ABA is clearly dependent on the state of flower induction which is different in plants growing on various photoperiods.


2014 ◽  
Vol 64 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Halina Kulikowska-Gulewska ◽  
Mariusz Cymerski ◽  
Joanna Czaplewska ◽  
Jan Kopcewicz

The endogenous content of IAA in the cotyledons of <i>Pharbitis nil</i> is low before and during the first half of the inductive 16-h-long dark period. From the 8th to the 12th hour the level of IAA increased and then again was going down at the end of a dark period. Exogenous IAA applied to the cotyledones before and during the first half of the inductive dark period inhibits flower bud formation. The application of IAA to shoot apex also resulted in the inhibition of flowering. Experiments with TIBA, an auxin polar transport inhibitor, and PCIB, an auxin action inhibitor, have shown that auxin polar transport in cotyledones and long-distance auxin transport from cotyledones to shoot apex play an important role in IAA inhibition of flower bud formation. It suggests that auxins play their role not only at the level of floral induction in cotyledones, but also in the later events of floral evocation and differentiation in shoot apex.


2000 ◽  
Vol 38 (1) ◽  
pp. 39-46 ◽  
Author(s):  
Keisuke YAMAZAKI ◽  
Yukitsugu ISHII ◽  
Yasuo KAMURO ◽  
Itsuo TANAKA

2013 ◽  
Vol 36 (1-2) ◽  
pp. 83-94 ◽  
Author(s):  
Franciszka Jaumień

The growth of trees sprayed in spring with chlormequat is weaker, and their elongation growth ends 2 - 3 weeks earlier than that of unsprayed trees. Trees with growth inhibited by chlormequat set flower buds on the spurs and in the subapical part of long shoots. The course of flower bud differentiation starts in the second half of July and is similar to that in the apple tree.


2013 ◽  
Vol 35 (2) ◽  
pp. 185-190 ◽  
Author(s):  
E. S. Hegazi ◽  
G. R. Stino

The modifying effect of growth regulators on bud burst, flower bud formation and sex expression in olives varies greatly according to cultivar, concentration and time of application. Cycocel 200, 500 mg/l, ethephon 200 mg/l and SADH 2000 mg/l stimulated bud burst and flower bud formation in the 'Blanquetta' cv. A noticeable increase in the percentage of perfect flowers was obtained by 100 mg/l of kinetin, and 200 mg/l of Cycocel in the cv. 'Picual', Ethephon 200/1, Cycocel 200 mg/l and SADH 2000 mg/l considerably increased the percentage of perfect flowers in the cvs 'Blanquetta', 'Serrana' and 'Picual'. Treatments at green cluster stage were not effective.


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