THE FLUORESCENT TREPONEMAL ANTIBODY ABSORPTION TEST FOR SYPHILIS: A COMPARISON WITH THE TREPONEMA PALLIDUM IMMOBILIZATION TEST AND THE FLUORESCENT TREPONEMAL ANTIBODY TEST

1968 ◽  
Vol 1 (10) ◽  
pp. 404-406 ◽  
Author(s):  
M. F. Garner ◽  
N. M. Grantham ◽  
P. J. Roeder
Keyword(s):  
Treponema Pallidum ◽  
Antibody Test ◽  
Absorption Test ◽  
Antibody Absorption

scholarly journals Evaluation of Two Immunoblot Assays and a Western Blot Assay for the Detection of Antisyphilis Immunoglobulin G Antibodies

2009 ◽  
Vol 17 (1) ◽  
pp. 183-184 ◽  
Author(s):  
Ryan J. Welch ◽  
Christine M. Litwin
Keyword(s):  
Western Blot ◽  
Immunoglobulin G ◽  
Treponema Pallidum ◽  
Absorption Test ◽  
Rapid Plasma Reagin ◽  
Western Blot Assay ◽  
Agglutination Assay ◽  
Antibody Absorption ◽  
Particle Agglutination

ABSTRACT In the present study, two immunoglobulin G (IgG) immunoblot assays and one IgG Western blot assay were compared to the rapid plasma reagin test (RPR), the fluorescent treponemal antibody absorption test (FTA-ABS), and the Treponema pallidum particle agglutination assay (TP-PA). The agreement levels of the Viramed, Virotech, and MarDx assays were 97.0%, 96.4%, and 99.4%, and the agreements of samples inconclusive by FTA-ABS and resolved by TP-PA were 91.7%, 83.3%, and 69.4%, respectively.

Neurosyphilis presenting as psychiatric symptoms: an unusual case report

2007 ◽  
Vol 19 (4) ◽  
pp. 251-253 ◽  
Author(s):  
Hasan Mirsal ◽  
Ayhan Kalyoncu ◽  
Özkan Pektaş ◽  
Mansur Beyazyürek
Keyword(s):  
Psychiatric Symptoms ◽  
Treponema Pallidum ◽  
Antibody Test ◽  
Risk Groups ◽  
Serological Tests ◽  
Case Presentation ◽  
Antibody Absorption ◽  
Gradual Loss ◽  
Neuropsychiatric Diseases ◽  
Organic Brain

Objective:Neurosyphilis remains a differential diagnosis for a wide variety of psychiatric syndromes, including dementia, mood disorders and psychosis. However, the incidence of neurosyphilis presenting initially with psychiatric symptomalogy is unclear. In this article, a clinical case is reported so as to illustrate some of the issues involved.Case presentation:A 33-year-old married man was admitted because of a depressive episode associated with somatic preoccupations and a gradual loss of ability to function a year prior to his admission. The symptoms described above raised the question of an organic brain syndrome associated with the psychotic depression. Some laboratory and additional examinations were performed. Serological tests for syphilis were positive for both the hemagglutination test, Treponema pallidum hemaglutination (TPHA), and the immunofluorescent antibody test, fluorescent treponemal antibody-absorption (FTA-ABS). Based on these findings, the diagnosis of neurosyphilis was made. The patient was treated with benzathine penicillin (2 400 000 units i.m. once a week) for 3 weeks and venlafaxine (150 mg daily) and olanzapine (5 mg daily). His depression disappeared gradually, and he was discharged in partial remission.Conclusion:High-risk groups such as patients with neuropsychiatric diseases should be screened with serological tests so as to prevent morbidity and help eliminate syphilis.

scholarly journals Problems affecting performance of the fluorescent treponemal antibody-absorption test for syphilis

1979 ◽  
Vol 9 (2) ◽  
pp. 163-166
Author(s):  
E F Hunter ◽  
M R Adams ◽  
L H Orrison ◽  
B J Pender ◽  
S A Larsen
Keyword(s):  
Treponema Pallidum ◽  
Immunoglobulin M ◽  
Immunofluorescent Staining ◽  
Heat Inactivation ◽  
Absorption Test ◽  
Test Results ◽  
False Positive Reaction ◽  
Antibody Absorption ◽  
Immunoglobin G

Immunofluorescent staining of Treponema pallidum was studied to clarify the effect of three factors on the results of the fluorescent treponemal antibody-absorption test: (i) heat inactivation of sera at 56 degrees C for 30 min before testing, (ii) use of multicircle slides, and (iii) tungsten illumination to visualize and assess unstained treponemes on reactive as well as nonreactive smears. It was found that serum inactivation before testing was not necessary for detection of immunoglobin G antibody, but an immunoglobulin M prozone was detected in unheated serum. On multicircle slides, it was demonstrated that a false-positive reaction could be obtained in 30 s at 37 and 25 degrees C if a smear where a nonreactive serum had been placed was crossed by a strongly reactive serum from another circle. Tungsten illumination proved necessary for correct assessment of unstained treponemes on all fluorescent treponemal antibody-aborption test smears, reactive or nonreactive. The possible role of these factors in incorrect fluorescent treponemal antibody-absorption test results is discussed.

scholarly journals AUTOMATION OF THE FLUORESCENT TREPONEMAL ANTIBODY-ABSORPTION TEST

1972 ◽  
Vol 20 (4) ◽  
pp. 255-260 ◽  
Author(s):  
GERALD I. KAUFMAN ◽  
JAMES F. NESTER
Keyword(s):  
Treponema Pallidum ◽  
Laser Scanner ◽  
Human Observer ◽  
Absorption Test ◽  
Optical Pattern Recognition ◽  
Antibody Absorption ◽  
Optical Pattern ◽  
Ion Laser ◽  
Complete Automation ◽  
Argon Ion

The feasibility of automating the decision-making tasks of the human observer in the microscopic determination of Fluorescent Treponemal Antibody-Absorption test results was investigated. An argon-ion laser scanner-optical pattern recognition computer instrument was utilized. The results indicate that the use of area, perimeter and (perimeter)2/area parameters allows the identification of Treponema pallidum as distinguished from other, nonspecific fluorescing objects. It was concluded that complete automation of the Fluorescent Treponemal Antibody-Absorption test is feasible with existing technology.

Immunoglobulin specificity for the fluorescent treponemal antibody-absorption test conjugate

1976 ◽  
Vol 4 (4) ◽  
pp. 338-342
Author(s):  
E F Hunter ◽  
S E Maddison ◽  
S A Larsen ◽  
M B Felker ◽  
J C Feeley
Keyword(s):  
Indirect Immunofluorescence ◽  
Immunoglobulin G ◽  
Human Immunoglobulin ◽  
Absorption Test ◽  
Human Immunoglobulin G ◽  
Antibody Absorption

Quantitative indirect immunofluorescence tests have demonstrated the predominance of immunoglobulin G antibody in most stages of syphilis. A class-specific anti-human immunoglobulin G conjugate is recommended to replace the present fluorescent treponemal antibody-absorption test conjugate. This would allow further definition and standardization of the fluorescent treponemal antibody-absorption test.

scholarly journals Human Hardjo leptospirosis detected in the Slovak Republic by using serum antibody absorption test

2019 ◽  
Vol 120 (03) ◽  
pp. 171-176
Author(s):  
P. Bakoss ◽  
H. Hudecova ◽  
J. Jarekova ◽  
J. Perzelova
Keyword(s):  
Slovak Republic ◽  
Serum Antibody ◽  
Absorption Test ◽  
Antibody Absorption
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