SCREENING THYROMETABOLIC DISORDERS USING TOTAL SERUM THYROXINE LEVEL

Abstract A new semiautomatic procedure for determination of total serum thyroxine was adapted to a kinetic analyzer, LKB 2086 Mark II. The ABA thyroxine assay kit was used, and although the assay procedure was modified, the advantage of small reagent volumes, short measuring times, and automation were retained. The results were analyzed off-line with a programmable desk-top calculator. The method has a precision of 7% (CV) and a sensitivity of 8 nmol/L. Values in sera from 64 patients analyzed by enzyme immunoassay and by radioimmunoassay correlated well (r = 0.975). One kit contains enough reagents for 500 assays, and an operator could do 25 samples (i.e., 64 assays) in about 4 h.

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A method for the direct estimation of total serum thyroxine

Irish Journal of Medical Science (1971 -) ◽

10.1007/bf02958789 ◽

1968 ◽

Vol 1 (9) ◽

pp. 395-404 ◽

Cited By ~ 2

Author(s):

P. P. A. Smyth ◽

D. K. O’Donovan

Keyword(s):

Total Serum ◽

Serum Thyroxine ◽

Direct Estimation

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Changes in Concentrations of Serum Thyroxine, Skin and Serum Guanine, and Total Serum Protein in the Masu Salmon during Smoltification.

NIPPON SUISAN GAKKAISHI ◽

10.2331/suisan.59.1225 ◽

1993 ◽

Vol 59 (7) ◽

pp. 1225-1229 ◽

Cited By ~ 2

Author(s):

Kazuhiro Ura ◽

Akihiko Hara ◽

Kohei Yamauchi

Keyword(s):

Serum Protein ◽

Masu Salmon ◽

Total Serum Protein ◽

Total Serum ◽

Serum Thyroxine

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Serum thyroxine level and cognitive decline in euthyroid older women

Neurology ◽

10.1212/wnl.58.7.1055 ◽

2002 ◽

Vol 58 (7) ◽

pp. 1055-1061 ◽

Cited By ~ 70

Author(s):

S. Volpato ◽

J. M. Guralnik ◽

L. P. Fried ◽

A. T. Remaley ◽

A. R. Cappola ◽

...

Keyword(s):

Cognitive Decline ◽

Older Women ◽

Serum Thyroxine ◽

Thyroxine Level

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SERUM THYROXINE DETERMINATION BY SATURATION ANALYSIS: A NEW METHOD AND STATISTICAL APPROACH

Acta Endocrinologica ◽

10.1530/acta.0.0680431 ◽

1971 ◽

Vol 68 (3) ◽

pp. 431-444 ◽

Cited By ~ 1

Author(s):

A. Burger ◽

J. R. Scherrer ◽

B. Miller ◽

M. B. Vallotton

Keyword(s):

Digital Computer ◽

High Speed ◽

Statistical Approach ◽

New Method ◽

Resolving Power ◽

Mouse Serum ◽

Total Serum ◽

Serum Thyroxine ◽

Technical Details ◽

First Time

ABSTRACT A new method for the measurement of total serum thyroxine (T4) by saturation analysis is presented along with technical details. Its main advantage over previously published methods is its sensitivity of 0.3 picomoles T4 (corresponding to 0.23 ng T4 or 152 pg iodine). It thus allows the measurement of serum thyroxine in very small volumes of human serum (7 μl) and, for the first time, in mouse serum. The use of Sephadex to extract thyroxine from the plasma gives more reproducible results and avoids the need to evaporate the extract to dryness, as in current methods. A high speed digital computer was used for handling data processing. A new statistical algorithm was built into a code called REGSAT which is extensively described. This procedure allowed unskewed predictions of thyroxine levels in the samples and an estimate of the standard curves resolving power.

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