Simultaneous Determination of Saponins and a Flavonoid from Aerial Parts of Zygophyllum coccineum L.

2012 ◽  
Vol 95 (3) ◽  
pp. 757-762
Author(s):  
Elham Amin ◽  
Yan-Hong Wang ◽  
Bharathi Avula ◽  
Seham S El-Hawary ◽  
Magda M Fathy ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Relevant Literature ◽  
Hplc Method ◽  
Desert Plant ◽  
Gradient System ◽  
Alcohol Extract ◽  
Triterpenoid Saponins ◽  
Wide Range ◽  
Acquity Uplc

Abstract Triterpenoid saponins are a class of glycosides with a wide range of bioactivities, which make them interesting research candidates. Zygophyllum coccineum is an Egyptian desert plant rich in triterpenoid saponins. Reviewing the relevant literature, no data concerning the HPLC or ultra-performance LC (UPLC) analysis of Zygophyllum content were found. This paper presents two methods, HPLC-UV and UPLC-UV-evaporative light scattering detector (ELSD)/MS, for the simultaneous determination of 10 compounds in the alcohol extract of Z. coccineum. The HPLC method uses a C18 column and water–acetonitrile (both containing 0.1% trifluoroacetic acid) gradient system. The separation was achieved within 32 min. The developed UPLC method simultaneously detects and quantifies the 10 compounds using an Acquity UPLC BEH Shield RP18 column and reagent alcohol–acetonitrile (80/20, v/v) and water (both containing 0.5% formic acid) gradient system within 14 min with UV, ELS, and MS detectors. The methods were used to analyze another species, Z. simplex, and results revealed a great variation between the secondary metabolite pattern of both species.

Validation of an HPLC Method for the Simultaneous Determination of trans-Lutein, (3R,3′R)-trans-Zeaxanthin, and (3R,3′S)-trans-Zeaxanthin

2013 ◽  
Vol 96 (3) ◽  
pp. 630-634 ◽  
Author(s):  
John R Warfield ◽  
Brandon J Lewis
Keyword(s):  
Simultaneous Determination ◽  
Chiral Separation ◽  
Solubility Limit ◽  
Hplc Method ◽  
Limiting Factor ◽  
Injection Solution ◽  
Chiral Column ◽  
Wide Range ◽  
Target Analyte

Abstract trans-Lutein, (3R,3′R)-trans-zeaxanthin, and (3R,3′S, meso)-trans-zeaxanthin are carotenoid isomers found in the human eye. Trans-lutein and trans-zeaxanthin can be separated under NP chromatographic conditions; however, (3R,3′R)-trans-zeaxanthin and (3R,3′S)-trans- zeaxanthin coelute and require chiral separation techniques. The validation of an HPLC analytical method for the simultaneous determination of trans-lutein, (3R,3′R)-trans-zeaxanthin, and (3R,3′S)-trans- zeaxanthin is described. The method uses a hexanes and 2-propanol gradient on a Daicel AD-H chiral column to separate the carotenoids for detection at 446 and 450 nm. The method was found suitable for use in quantifying the three analytes over a wide range of concentrations with the limiting factor being the upper solubility limit of 14 μg of (3R,3′S)-trans-zeaxanthin/mL of the injection solution of hexane–2-propanol (95 + 5, v/v). The precision of the method, expressed by the coefficient of variation, was <5% for the analytes when triplicate injections were used. The average recoveries ranged between 95 and 103% for standards that were 50 to 200% of the target analyte level. The LOQ in the injection solution was determined to be between 1 and 6 ng/mL for the analytes. The method is valid for the separation and quantification of trans-lutein, (3R,3′R)-trans-zeaxanthin, and (3R,3′S)-trans-zeaxanthin.

RP-HPLC method development for simultaneous determination of the drugs Ramipril and Amlodipine

2012 ◽  
Vol 2 (2) ◽  
pp. 364-367 ◽  
Author(s):  
Saida Naik Dheeravath ◽  
◽  
Kasani Ramadevi ◽  
Zilla Saraswathi ◽  
Dheeravath Maniklal ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Method Development ◽  
Hplc Method ◽  
Rp Hplc ◽  
Hplc Method Development

Simultaneous Determination of Chloroquine and Pyrimethamine with Cetirizine in an Active Form and Human Serum by RP-HPLC

2021 ◽  
Author(s):  
Hina Shamshad ◽  
Ali Sayqal ◽  
Jahan Zeb ◽  
Agha Zeeshan Mirza
Keyword(s):  
Human Serum ◽  
Simultaneous Determination ◽  
Active Form ◽  
Internal Standard ◽  
Hplc Method ◽  
Serum Samples ◽  
Rp Hplc ◽  
Cetirizine Hydrochloride ◽  
Bulk Drug

Abstract A simple, accurate and precise RP-HPLC method was developed for the simultaneous determination of chloroquine, pyrimethamine and cetirizine hydrochloride concentrations in bulk drug and human serum. The assay was performed using a mobile phase of methanol: water (70:30) at pH of 2.8 ± 0.05 on the Purospher C-18 column with UV detection at 230 nm and rosuvastatin used as an internal standard. The retention times observed for chloroquine, pyrimethamine and cetirizine hydrochloride were 3.5, 2.5 and 5.5 minutes, respectively. The method was found to be specific for the assayed drugs showing a linear response in the concentration range of 1–100 μg mL−1 with coefficients of determination values of (r = 0.999). The method was developed and validated according to ICH guidelines. The method was used to monitor the serum samples and was found to be sensitive for therapeutic purposes, showing the potential to be a useful tool for routine analysis in laboratories.

A HPLC method for the simultaneous determination of seven anthracyclines

2005 ◽  
Vol 39 (1-2) ◽  
pp. 305-309 ◽  
Author(s):  
Irinel Badea ◽  
Lelia Lazăr ◽  
Dana Moja ◽  
Daniela Nicolescu ◽  
Aurel Tudose
Keyword(s):  
Simultaneous Determination ◽  
Hplc Method

scholarly journals Simultaneous Determination of Preservatives (Methyl Paraben and Propyl Paraben) in Sucralfate Suspension Using High Performance Liquid Chromatography

2011 ◽  
Vol 8 (1) ◽  
pp. 340-346 ◽  
Author(s):  
Rajesh M. Kashid ◽  
Santosh G. Singh ◽  
Shrawan Singh
Keyword(s):  
High Performance Liquid Chromatography ◽  
Simultaneous Determination ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Hplc Method ◽  
Reversed Phase Hplc ◽  
Methyl Paraben ◽  
Propyl Paraben

A reversed phase HPLC method that allows the separation and simultaneous determination of the preservatives methyl paraben (M.P.) and propyl paraben (P.P.) is described. The separations were effected by using an initial mobile phase of water: acetonitrile (50:50) on Inertsil C18 to elute P.P. and M.P. The detector wavelength was set at 205 nm. Under these conditions, separation of the two components was achieved in less than 10 min. Analytical characteristics of the separation such as precision, specificity, linear range and reproducibility were evaluated. The developed method was applied for the determination of preservative M.P. and P.P. at concentration of 0.01 mg/mL and 0.1 mg/mL respectively. The method was successfully used for determining both compounds in sucralfate suspension.

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