scholarly journals Anther Culture as a Supplementary Tool for Rice Breeding

2018 ◽  
Author(s):  
D.M. Ruwani G. Mayakaduwa ◽  
Tara D. Silva
Keyword(s):  
Anther Culture ◽  
Rice Breeding

scholarly journals Doubled Haploids in Rice Improvement: Approaches, Applications, and Future Prospects

2021 ◽  
pp. 425-447
Author(s):  
Sanghamitra Samantaray ◽  
Jauhar Ali ◽  
Katrina L. C. Nicolas ◽  
Jawahar Lal Katara ◽  
Ram Lakhan Verma ◽  
...  
Keyword(s):  
Anther Culture ◽  
Doubled Haploids ◽  
Green Plant ◽  
Inbred Lines ◽  
Rice Breeding ◽  
Future Prospects ◽  
Crop Species ◽  
Precision Breeding ◽  
Short Period ◽  
Rice Improvement

AbstractExploitation of biotechnological tools in conventional breeding strategies is the need of the hour for overcoming limitations in rice production and productivity. In addition, improvement in quantity and quality along with resistance to climatic and disease stress in rice require immediate attention. Anther culture has proven its efficiency by instantaneously fixing homozygosity through diploidization of regenerated haploid plants. Therefore, androgenesis provides an efficient platform for developing inbred lines in a short period of time. Although anther culture shows its efficiency in speeding up breeding in several crop species, including rice, associated limitations still prevent the exploitation of its optimum potential. Although anther culture is well exploited in japonica rice breeding, its application in indica rice is limited because of inherent recalcitrant genetic backgrounds. The success of anther culture is determined by several factors that limit the efficiency of androgenesis. Identified constraints are early anther necrosis, poor-callus response, and proliferation, and low green-plant regeneration, along with the most frustrating albinism associated with indica rice, which has been considerably clarified. This chapter details the method of androgenesis and scope for improving the applicability of anther culture producing doubled haploids of rice in order to use it as a complementary tool for precision breeding.

Anther culture as a rice breeding tool: a profitable investment

2008 ◽  
pp. 511-518
Author(s):  
L. R. Sanint ◽  
C. P. Martínez ◽  
Z. Lentini
Keyword(s):  
Anther Culture ◽  
Rice Breeding

scholarly journals Kultur Antera untuk Percepatan Perakitan Varietas Padi di Indonesia

2016 ◽  
Vol 8 (2) ◽  
pp. 78 ◽  
Author(s):  
Iswari S Dewi ◽  
Bambang S. Purwoko
Keyword(s):  
Anther Culture ◽  
Aluminum Toxicity ◽  
Selection Process ◽  
Indica Rice ◽  
Doubled Haploids ◽  
Population Increase ◽  
Rice Breeding ◽  
Male Sterile ◽  
Breeding Method ◽  
Biotic Stresses

<p>Rice is a staple<br />food crop in Indonesia, while the need is increasing due to<br />high rice consumption as well as population increase. The<br />problems can be solved through increase of national rice<br />production. Productivity of lowland and upland should be<br />increased intensively and other potential dry area outside<br />Java and Bali Islands should be considered for extending the<br />area of production. Recently, high yielding variety such as<br />semi dwarf variety, hybrid rice, and new plant type of rice<br />were being developed by Indonesian breeders. However,<br />new method is needed to complement conventional<br />breeding method in order to accelerate rice breeding.<br />Anther culture is one of in vitro culture techniques that can<br />be used to accelerate the obtainment of pure lines through<br />doubled-haploids (DHs) regenerated at first generation of<br />culture for less than one year. Thus, application of anther<br />culture in conventional breeding will increase the efficiency<br />of selection process as well as reducing the cost for labour,<br />land and breeder’s time. The obtainment of green plantlets<br />derived from anther culture of indica rice subspecies has<br />been improved by the addition of 1 mM putrescine into<br />induction and regeneration media. Recently, several upland<br />rice lines tolerant to abiotic stresses (i.e. low light intensity<br />and aluminum toxicity) and biotic stresses (i.e. leaf and<br />neck blast), several lowland rice/paddy lines tolerant to<br />biotic stresses (i.e. bacterial leaf blight and blast), and<br />several hybrid parental lines (i.e. male sterile, maintainer<br />and restorer) were obtained in 2-3 years from several rice<br />breeding program involving anther culture. However,<br />potential use anther culture to provide unique genetic<br />material for mapping populations for use in functional<br />genomics and molecular breeding has not been explored.<br />The results indicated that anther culture is a feasible<br />technology that can be used for accelerating rice breeding<br />program in Indonesia.</p>

Some influence of the hormonal components in nutrient medium onthe induction of novel structures when obtaining rapeseed haploids through in vitro culture

2018 ◽  
pp. 30-37
Keyword(s):  
Anther Culture ◽  
Hormonal Regulation ◽  
Control Plant ◽  
Pollen Grains ◽  
Control Treatment ◽  
Basic Medium ◽  
Nutrient Media ◽  
Oilseed Crops ◽  
Wide Range

Growth regulators, phytohormones, both natural and artificial, are the main means to control plant ontogenesis. They are involved in regulating the processes of cell differentiation and cell divisions, the formation of tissues and organs, the changes in the rate of growth and development, the duration of the certain stages of ontogenesis. The main classes of phytohormones used in plant biotechnology, in particular, in the induction of haploid structures, are auxins and cytokinins. The mechanism of action of phytohormones on a cell is rather complicated and may have a different character. Understanding the characteristics of the action of phytohormones is complicated by the fact that the system of hormonal regulation of plant life is multicomponent. This is manifested in the fact that the same physiological process is most often influenced not by one, but by several phytohormones, covering a wide range of aspects of cell metabolism. In connection with the foregoing, the purpose of our work was to test a set of nutrient media with different basic composition and different proportions of phytohormones to determine the patterns of their influence on the processes of haploid structure induction in rape anther culture using accessions, developed at the Institute of Oilseed Crops NAAS. The material used was two accessions of winter rapeseed (No. 1 and No. 2) and one sample of spring rapeseed, provided by the Rapeseed Breeding laboratory of the Institute of Oilseed Crops. Incised inflorescences were kept against the background of low temperature of 6–8 ° C for several days, and then, under aseptic conditions, anthers with unripe pollen grains were isolated and planted on nutrient media differing in both basic mineral composition and content of phytohormones. MS (Murashige & Skoog 1962) and B5 (Gamborg et al 1968) media were used as basic media. Phytohormones were added to the basic media in various combinations – BA, 2,4-D, NAA at the concentrations of 0.1-0.6 mg/l. In each treatment up to 300 anthers were cultivated. Differences between treatments were evaluated using standard t-test. Studies have shown that in the anther culture of rapeseed on the tested nutrient media, morphogenic structures of different types (embryoids and callus) were originated. Synthetic auxin 2,4-D, regardless of the composition of the basic medium, caused the formation of structures of both types, though with a low frequency. Phytohormone BA of the cytokinin type had a similar effect. In this case, the frequency of structures was slightly higher, and the developed structures were represented mainly by embryoids. The joint action of cytokinin and auxin was the most favorable for the initiation of morphogenic structures. Such combination of phytohormones caused the formation of these structures with a frequency of 24.5-14.7% in the studied genotypes of winter rape. A similar effect of phytohormones on the induction and development of morphogenic structures was also observed in spring rape. In this case, a single basic MS medium was used. The experiment included treatments where phytohormones were absent (control), as well as various combinations of auxin and cytokinin. In the control treatment, the formation of new structures was not noted. In treatments with phytohormones, in addition to the medium with the combination of auxin and cytokinin, the medium in which only cytokinin was present was also rather effective. The treatment in which the action of auxin 2,4-D was combined with the action of another auxin, NAA, turned out to be practically ineffective. Thus, it was found that for the induction of morphogenic structures from microspores in rape anther culture of the tested genotypes, the combination of cytokinin with auxin, or the use of only single cytokinin BA without other phytohormones, had the most positive effect.

Effect of a Modified Potato Medium on Anther Culture of Wheat 1

Crop Science ◽  
1987 ◽  
Vol 27 (2) ◽  
pp. 351-354 ◽  
Author(s):  
J. E. Marburger ◽  
D. J. Sammons ◽  
G. W. Schaeffer
Keyword(s):  
Anther Culture

Correlation of traits and their variability in rice breeding

2018 ◽  
Vol 1 (74) ◽  
pp. 25-32
Author(s):  
Ruslan Dzhamirze ◽  
◽  
Nadezhda Ostapenko ◽  
Keyword(s):  
Rice Breeding

Plant Type as a Rice Breeding Objective 1

Crop Science ◽  
1964 ◽  
Vol 4 (1) ◽  
pp. 13-15 ◽  
Author(s):  
Peter R. Jennings
Keyword(s):  
Rice Breeding ◽  
Plant Type ◽  
Breeding Objective
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