scholarly journals Doubled Haploids in Rice Improvement: Approaches, Applications, and Future Prospects

2021 ◽  
pp. 425-447
Author(s):  
Sanghamitra Samantaray ◽  
Jauhar Ali ◽  
Katrina L. C. Nicolas ◽  
Jawahar Lal Katara ◽  
Ram Lakhan Verma ◽  
...  
Keyword(s):  
Anther Culture ◽  
Doubled Haploids ◽  
Green Plant ◽  
Inbred Lines ◽  
Rice Breeding ◽  
Future Prospects ◽  
Crop Species ◽  
Precision Breeding ◽  
Short Period ◽  
Rice Improvement

AbstractExploitation of biotechnological tools in conventional breeding strategies is the need of the hour for overcoming limitations in rice production and productivity. In addition, improvement in quantity and quality along with resistance to climatic and disease stress in rice require immediate attention. Anther culture has proven its efficiency by instantaneously fixing homozygosity through diploidization of regenerated haploid plants. Therefore, androgenesis provides an efficient platform for developing inbred lines in a short period of time. Although anther culture shows its efficiency in speeding up breeding in several crop species, including rice, associated limitations still prevent the exploitation of its optimum potential. Although anther culture is well exploited in japonica rice breeding, its application in indica rice is limited because of inherent recalcitrant genetic backgrounds. The success of anther culture is determined by several factors that limit the efficiency of androgenesis. Identified constraints are early anther necrosis, poor-callus response, and proliferation, and low green-plant regeneration, along with the most frustrating albinism associated with indica rice, which has been considerably clarified. This chapter details the method of androgenesis and scope for improving the applicability of anther culture producing doubled haploids of rice in order to use it as a complementary tool for precision breeding.

Genotype and Environment Strongly Influence Barley Anther Culture Response Using Australian Genotypes

1993 ◽  
Vol 41 (2) ◽  
pp. 227 ◽  
Author(s):  
SJ Logue ◽  
LC Giles ◽  
DHB Sparrow
Keyword(s):  
Anther Culture ◽  
Doubled Haploids ◽  
Growth Conditions ◽  
Green Plant ◽  
Optimal Conditions ◽  
Donor Plant ◽  
Breeding Programs ◽  
Barley Cultivars ◽  
Large Numbers ◽  
Anther Culture Response

A screening of several Australian barley cultivars of commercial interest has identified a number of genotypes that respond well to anther culture, with average levels of green plant regeneration between 23 and 134 plants/100 anthers cultured. Donor plant growth conditions have a large impact on anther culture response and, although optimal conditions for specific genotypes could possibly be identified, it is likely to be more effective for the production of large numbers of doubled haploids to settle for a broadly acceptable environment. Recent advances in methodology and the identification of responsive genotypes makes anther culture a feasible procedure for Australian barley breeding programs.

Diallel analysis of barley anther culture response

Genome ◽  
1988 ◽  
Vol 30 (2) ◽  
pp. 152-157 ◽  
Author(s):  
W. Powell
Keyword(s):  
Anther Culture ◽  
Doubled Haploids ◽  
Diallel Analysis ◽  
Green Plant ◽  
Plant Production ◽  
Microspore Development ◽  
Culture Techniques ◽  
Plantlet Formation ◽  
Breeding Programmes ◽  
Anther Culture Response

The genetics of barley microspore development in culture was examined by means of diallel analysis. The frequency of microspore derived green and albino plant production was shown to be under genetic control. This genotypic limitation to microspore development will limit the application of anther culture techniques to barley breeding programmes. However, significant additive genetic effects were detected for the characters measured and indicate that the frequency of green plant regeneration may be improved by the hybridization of suitable parents. Significant reciprocal differences were also detected and indicate that the direction of the cross is important in determining microspore development. An embryogenic route to green plantlet formation was observed in a number of genotypes in the diallel experiment. The implications of these findings for barley improvement and genetics are discussed.Key words: doubled haploids, barley, anther culture, microspore, embryoid.

Responsiveness to anther culture in cultivars and F1 crosses of spring wheat

1993 ◽  
Vol 73 (3) ◽  
pp. 777-783 ◽  
Author(s):  
P. Masojc ◽  
O. M. Lukow ◽  
R. I. H. McKenzie ◽  
N. K. Howes
Keyword(s):  
Anther Culture ◽  
Spring Wheat ◽  
Doubled Haploids ◽  
Green Plant ◽  
Donor Plant ◽  
Albino Plants ◽  
Ability To Regenerate ◽  
Embryoid Formation ◽  
N6 Medium ◽  
Regenerated Plant

Anther culturability of 43 cultivars and 6 F1 crosses representing different quality classes of spring wheat was studied using a glucose-containing, modified N6 medium (HNG). Generally high pollen embryoid formation (up to 111 embryoids per 100 anthers) was associated with lower green plant regeneration (up to 9.1 green plantlets per 100 embryoids) frequencies and a high proportion (63% on average) of albino plants. Anther response was found to be strongly affected by the genotype of the donor plants. Seven of the screened cultivars yielded more than one green regenerated plant per 100 anthers. The most responsive cultivars were Veery #2 (6.2), ST 6 (4.0), and Leader (3.6). No ability to regenerate green plantlets was shown by 10 of the genotypes. Anther responsiveness of F1 progenies as compared with the parental cultivars were different in each cross. Differences found between reciprocal crosses suggest that the cytoplasm of a donor plant may affect anther response. Haploid plants constituted 45% of the anther derived regenerants, while the remaining part was divided into 29% of spontaneous diploids and 26% of plants with abnormal chromosome numbers. Key words: Triticum aestivum, anther culture, doubled haploids, wheat

Low-Dose Gamma-Irradiation Promotes Wheat Anther Culture Response

1991 ◽  
Vol 39 (5) ◽  
pp. 467 ◽  
Author(s):  
XL Ding ◽  
DJ Luckett ◽  
NL Darvey
Keyword(s):  
Gamma Radiation ◽  
Gamma Irradiation ◽  
Anther Culture ◽  
Low Dose ◽  
Doubled Haploids ◽  
Low Frequency ◽  
Green Plant ◽  
Cold Pretreatment ◽  
60Co Source ◽  
Anther Culture Response

This study examined the anther culture response of wheat to different doses of gamma radiation, and the interaction of radiation dose with a cold pretreatment of ears stored prior to culturing. The cultivars Grebe and Kite were chosen on the basis of their anther culture response, Grebe being highly responsive and Kite being non-responsive. Spikes of the two cultivars were exposed to various levels of gamma radiation (60Co source) ranging from 0 to 10 Gy (0.53 Gy min-1) before anthers were plated on an agarose-solidified medium. For Grebe, doses of 1, 3 and 5 Gy resulted in more embryoids, higher green plant regeneration, and a greater number of spontaneously doubled haploids (DH), than in the non-irradiated control. The response in Kite was similar but less pronounced. The higher doses of radiation (7 and 10 Gy), however, were detrimental in both cultivars and at 10 Gy no embryoids or regenerants were produced. Anthers subjected to a cold pretreatment prior to irradiation responded significantly less than those cultured fresh. This study indicated that low-dose gamma irradiation of fresh explants can significantly improve regeneration from anther cultures in wheat and may stimulate a low frequency of regeneration in an otherwise non-responsive cultivar.

The application of n-butanol improves embryo and green plant production in anther culture of Australian wheat (Triticum aestivum L.) genotypes

2011 ◽  
Vol 62 (10) ◽  
pp. 813 ◽  
Author(s):  
Sue Broughton
Keyword(s):  
Triticum Aestivum ◽  
Anther Culture ◽  
Doubled Haploids ◽  
Triticum Aestivum L ◽  
Green Plant ◽  
Plant Production ◽  
Wheat Cultivars ◽  
Green Plants ◽  
Australian Wheat ◽  
New Treatments

The objective of this study was to improve the production from anther culture of embryos and green plants in Australian spring wheat genotypes by testing new treatments such as n-butanol, as well as other protocol modifications. To date, the use of n-butanol to enhance embryogenesis has only been tested in two European wheat cultivars; this is the first study which demonstrates its application across a range of breeding crosses. A 5-h treatment using 0.1 or 0.2% (v/v) n-butanol following anther pretreatment on a solid mannitol medium significantly improved the production of embryos, green plants and doubled haploids in a range of Australian wheat crosses and varieties. Green plant production increased between 3- and 6-fold in the crosses Yitpi/2*Bumper, Tammarin Rock/2*Bumper and Tammarin Rock/2*Magenta. The addition of calcium (Ca) and macronutrients to the mannitol pretreatment medium also significantly improved the number of embryos and green plants in varieties and crosses, but only when used in combination with n-butanol treatment. A factorial experiment with four varieties and two treatments (n-butanol and Ca/macronutrients) revealed significant interactions between treatments and genotype. In three of the four varieties, the application of n-butanol resulted in significant increases in embryos and green plants with either pretreatment medium although the best results were obtained with Ca and macronutrients in the pretreatment medium, with 200, 193 and 52 green plants per 100 anthers obtained for Bumper, Gladius and Magenta, respectively. In the variety Fortune however, n-butanol treatment did not improve embryo or green plant production unless it was combined with Ca and macronutrients in the pretreatment medium and then there were dramatic improvements; from 0 to 27 green plants per 100 anthers.

scholarly journals Kultur Antera untuk Percepatan Perakitan Varietas Padi di Indonesia

2016 ◽  
Vol 8 (2) ◽  
pp. 78 ◽  
Author(s):  
Iswari S Dewi ◽  
Bambang S. Purwoko
Keyword(s):  
Anther Culture ◽  
Aluminum Toxicity ◽  
Selection Process ◽  
Indica Rice ◽  
Doubled Haploids ◽  
Population Increase ◽  
Rice Breeding ◽  
Male Sterile ◽  
Breeding Method ◽  
Biotic Stresses

<p>Rice is a staple<br />food crop in Indonesia, while the need is increasing due to<br />high rice consumption as well as population increase. The<br />problems can be solved through increase of national rice<br />production. Productivity of lowland and upland should be<br />increased intensively and other potential dry area outside<br />Java and Bali Islands should be considered for extending the<br />area of production. Recently, high yielding variety such as<br />semi dwarf variety, hybrid rice, and new plant type of rice<br />were being developed by Indonesian breeders. However,<br />new method is needed to complement conventional<br />breeding method in order to accelerate rice breeding.<br />Anther culture is one of in vitro culture techniques that can<br />be used to accelerate the obtainment of pure lines through<br />doubled-haploids (DHs) regenerated at first generation of<br />culture for less than one year. Thus, application of anther<br />culture in conventional breeding will increase the efficiency<br />of selection process as well as reducing the cost for labour,<br />land and breeder’s time. The obtainment of green plantlets<br />derived from anther culture of indica rice subspecies has<br />been improved by the addition of 1 mM putrescine into<br />induction and regeneration media. Recently, several upland<br />rice lines tolerant to abiotic stresses (i.e. low light intensity<br />and aluminum toxicity) and biotic stresses (i.e. leaf and<br />neck blast), several lowland rice/paddy lines tolerant to<br />biotic stresses (i.e. bacterial leaf blight and blast), and<br />several hybrid parental lines (i.e. male sterile, maintainer<br />and restorer) were obtained in 2-3 years from several rice<br />breeding program involving anther culture. However,<br />potential use anther culture to provide unique genetic<br />material for mapping populations for use in functional<br />genomics and molecular breeding has not been explored.<br />The results indicated that anther culture is a feasible<br />technology that can be used for accelerating rice breeding<br />program in Indonesia.</p>

The genetic framework of plant breeding

1981 ◽  
Vol 292 (1062) ◽  
pp. 407-419 ◽  
Keyword(s):  
Plant Breeding ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Single Seed Descent ◽  
Crop Species ◽  
Biometrical Genetics ◽  
Mendelian Genetics ◽  
Linkage Disequilibria ◽  
Random Samples

The phenotypic variation that the breeder must manipulate to produce improved genotypes typically contains contributions from both heritable and non-heritable sources as well as from interactions between them. The totality of this variation can be understood only in terms of a methodology such as that of biometrical genetics - an extension of classical Mendelian genetics that retains all of its analytical, interpretative and predictive powers but only in respect of the net or summed effects of all contributing gene loci. In biometrical genetics the statistics that describe the phenotypic distributions are themselves completely described by heritable components based on the known types of gene action and interaction in combination with nonheritable components defined by the statistical properties of the experimental design. Biometrical genetics provides a framework for investigating the genetical basis and justification for current plant breeding strategies that are typified by the production of F 1 hybrids at one extreme and recombinant inbred lines at the other. From the early generations of a cross it can extract estimates of the heritable components of the phenotypic distributions that provide all the information required to interpret the cause of F 1 heterosis and predict the properties of any generation that can subsequently be derived from the cross. Applications to crosses in experimental and crop species show that true overdominance is not a cause of F 1 heterosis, although spurious overdominance arising from linkage disequilibria and non-allelic interactions can be. Predictions of the phenotypic distributions and ranges of recombinant inbred lines that should be extractable from these crosses are confirmed by observations made on random samples of inbred families produced from them by single seed descent. Within these samples, recombinant inbred lines superior to existing inbred lines and their F 1 hybrids are observed with the predicted frequencies.

scholarly journals Influence of Detasseling Methods on Seed Yield of Some Parent Inbred Lines of Turda Maize Hybrids

Agronomy ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 729
Author(s):  
Alexandru Bogdan Ghețe ◽  
Voichița Haș ◽  
Roxana Vidican ◽  
Ana Copândean ◽  
Ovidiu Ranta ◽  
...  
Keyword(s):  
Seed Production ◽  
Seed Yield ◽  
Weather Conditions ◽  
Inbred Lines ◽  
Plant Performance ◽  
Yield Response ◽  
Genetic Purity ◽  
Short Period ◽  
Number Of Leaves ◽  
Hybrid Maize

During the process of maize seed production, in order to ensure the genetic purity of parental forms of hybrid maize, an important work performed is the removal of male inflorescences from plants on mother rows. Hand detasseling has high precision but is labor-intensive. Mechanical detasseling offers the possibility to cover large acreages in a short period of time, but the number of leaves removed has a varying influence on plant performance and seed yield. The aim of this study was to simulate three types of damages on plants similar to those induced through mechanical detasseling and to assess the effects for five inbred lines during the course of three years. Results show that when tassels alone were removed, the average seed yield decreased an average of 4–21%. When two leaves were removed with the tassel, yield decreased an average of 22–31%, while when plants were cut above the main ear, seed yield decreased an average of 31–66%. Environmental conditions influenced seed yield, especially high temperatures during flowering. Yield response to tassel and leaves removal varied between the inbred lines. Genotype controls maize ear and kernel characters, while environmental factors exercise a strong influence on seed yield, due to the succession of years with contrasting weather conditions in a key phenophase. Within the trend of full mechanization in agriculture, identification of inbred lines that cope better with plant damage can assist in optimizing seed production.

scholarly journals Applications of molecular markers in Genetic Diversity Studies of maize

2020 ◽  
Vol 37 (1) ◽  
pp. 101-108
Author(s):  
Degife Asefa Zebire
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Inbred Lines ◽  
Hybrid Breeding ◽  
Diversity Analysis ◽  
Crop Species ◽  
Heterotic Groups ◽  
Genetic Diversity Analysis ◽  
Diversity Studies ◽  
Genetic Diversity Study

Molecular markers are efficient for exploiting variations in genotypes as they are not influenced by environmental factors and also speed up breeding programs. They are used to detect large numbers of distinct divergence between genotypes at the DNA level. Genetic diversity study helps to estimate the relationship between inbred lines to make the best hybrid combinations. Lines which are clustered in different heterotic groups are considered as the best hybrid combinations to carry out further breeding activities. Molecular markers are used to meet a number of objectives, including genetic diversity analysis and prediction of hybrid performances in divergent crop species. Agro-morphological and molecular markers have been utilized to study genetic diversity so far. In maize, the uses of molecular markers are important for the evaluation of genetic diversity of inbred lines and in clustering them into heterotic groups. These markers determine genetic similarity of the lines and are used to assess the genetic diversity of maize. Molecular markers have proven valuable for genetic diversity analysis of many crop species and genetically diverse lines are important to improve hybrid breeding. Keyword: Molecular marker; Genetic diversity; Genetic variation, Diversity Array technology; cluster analysis

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