scholarly journals Ontogeny of the Human Pancreas

2019 ◽  
Author(s):  
Alexandra E. Proshchina ◽  
Yuliya S. Krivova ◽  
Larisa E. Gurevich ◽  
Valeriy M. Barabanov ◽  
Dmitriy A. Otlyga ◽  
...  
Keyword(s):  
Human Pancreas

IMMUNOLOGICAL REACTIVITY OF INSULIN IN HUMAN SERUM

1966 ◽  
Vol 53 (4) ◽  
pp. 673-680 ◽  
Author(s):  
Torsten Deckert ◽  
Kai R. Jorgensen
Keyword(s):  
Normal Subjects ◽  
The Other ◽  
Human Pancreas ◽  
Porcine Pancreas ◽  
Crystalline Insulin ◽  
Endogenous Insulin ◽  
Significant Difference ◽  
Human Sera ◽  
Normal Human ◽  
The One

ABSTRACT The purpose of this study was to investigate whether a difference could be demonstrated between crystalline insulin extracted from normal human pancreas, and crystalline insulin extracted from bovine and porcine pancreas. Using Hales & Randle's (1963) immunoassay no immunological differences could be demonstrated between human and pig insulin. On the other hand, a significant difference was found, between pig and ox insulin. An attempt was also made to determine whether an immunological difference could be demonstrated between crystalline pig insulin and crystalline human insulin from non diabetic subjects on the one hand and endogenous, circulating insulin from normal subjects, obese subjects and diabetic subjects on the other. No such difference was found. From these experiments it is concluded that endogenous insulin in normal, obese and diabetic human sera is immunologically identical with human, crystalline insulin from non diabetic subjects and crystalline pig insulin.

scholarly journals Hypothermic oxygenated Machine Perfusion of the Human Pancreas for Clinical Islet Isolation: A Prospective Feasibility Study

2021 ◽  
Author(s):  
Jason B. Doppenberg ◽  
Marjolein Leemkuil ◽  
Marten A. Engelse ◽  
Christina Krikke ◽  
Eelco J.P. de Koning ◽  
...  
Keyword(s):  
Feasibility Study ◽  
Human Pancreas ◽  
Islet Isolation ◽  
Machine Perfusion

Local islet remodelling associated with duct lesion–islet complex in adult human pancreas

Diabetologia ◽  
2021 ◽  
Author(s):  
Yu-Wen Tien ◽  
Hung-Jen Chien ◽  
Tsai-Chen Chiang ◽  
Mei-Hsin Chung ◽  
Chih-Yuan Lee ◽  
...  
Keyword(s):  
Human Pancreas ◽  
Adult Human

scholarly journals Trypsin Inhibitor from Human Pancreas and Pancreatic Juice

1974 ◽  
Vol 249 (7) ◽  
pp. 2235-2242
Author(s):  
Merton H. Pubols ◽  
Diana C. Bartelt ◽  
Lewis J. Greene
Keyword(s):  
Pancreatic Juice ◽  
Trypsin Inhibitor ◽  
Human Pancreas

Lipolytic Enzymes of the Human Pancreas

Enzyme ◽  
1982 ◽  
Vol 28 (4) ◽  
pp. 336-342 ◽  
Author(s):  
J. G. Meyer-Bertenrath
Keyword(s):  
Human Pancreas ◽  
Lipolytic Enzymes

Expression and distribution of the Na+- HCO 3 − cotransporter in human pancreas

1999 ◽  
Vol 277 (2) ◽  
pp. G487-G494 ◽  
Author(s):  
Christopher R. Marino ◽  
Virginia Jeanes ◽  
Walter F. Boron ◽  
Bernhard M. Schmitt
Keyword(s):  
Northern Blot ◽  
Islet Cells ◽  
Rat Kidney ◽  
Physiological Role ◽  
Human Pancreas ◽  
Transmembrane Conductance Regulator ◽  
Transmembrane Conductance ◽  
Cellular Mechanisms ◽  
Double Labeling ◽  
Normal Human

The cellular mechanisms of [Formula: see text] secretion in the human pancreas are unclear. Expression of a Na+-[Formula: see text]cotransporter (NBC) mRNA has been observed recently, but the distribution and physiological role of the NBC protein are not known. Here we examined the expression and localization of NBC in human pancreas by Northern blot, immunoblot, and immunofluorescence microscopy. Rat kidney NBC probes detected a single 9.5-kb band by Northern blot. On immunoblots, two polyclonal antisera directed against different epitopes of rat kidney NBC identified a single ∼130-kDa protein. In cryosections of normal human pancreas, both antisera labeled basolateral membranes of large, morphologically identifiable ducts and produced a distinct labeling pattern in the remainder of the parenchyma. In double-labeling experiments, NBC immunoreactivity in the parenchyma colocalized with the Na+-K+pump, a basolateral marker. In contrast, NBC and cystic fibrosis transmembrane conductance regulator, an apical membrane marker, were detected within the same histological structures but at different subcellular localizations. The NBC antisera did not label acinar or islet cells. Our observations suggest that secretion of[Formula: see text] by human pancreatic duct cells involves the basolateral uptake of Na+and[Formula: see text] via NBC, an electrogenic Na+-[Formula: see text]cotransporter.

Advanced qRT-PCR Technology Allows Detection of the Cholecystokinin 1 Receptor (CCK1R) Expression in Human Pancreas

Pancreas ◽  
2005 ◽  
Vol 31 (4) ◽  
pp. 325-331 ◽  
Author(s):  
Jose Galindo ◽  
Neil Jones ◽  
Garth L Powell ◽  
Simon J Hollingsworth ◽  
Nigel Shankley
Keyword(s):  
Human Pancreas ◽  
Qrt Pcr ◽  
Pcr Technology

Evaluation of a human pancreas-specific antigen by enzyme-linked immunosorbent assay

1981 ◽  
Vol 117 (3) ◽  
pp. 251-258 ◽  
Author(s):  
Rueyming Loor ◽  
Manabu Kuriyama ◽  
Mary Lou Manzo ◽  
Hideo Inaji ◽  
Harold O. Douglass ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Specific Antigen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Pancreas

scholarly journals The immunological characterization of several human ribonucleases by using primary binding tests

1977 ◽  
Vol 163 (3) ◽  
pp. 419-426 ◽  
Author(s):  
E A Neuwelt ◽  
M Schmukler ◽  
M S Niziak ◽  
P B Jewett ◽  
C C Levy
Keyword(s):  
Human Plasma ◽  
Cross Reaction ◽  
The Other ◽  
Human Pancreas ◽  
Human Tissues ◽  
Antigenic Difference ◽  
Immunological Characterization ◽  
Similarities And Differences

RNAases (ribonucleases), purified from four human tissues, as well as bovine pancreatic RNAase (RNAase A), were studied by immunodiffusion methods and by two different primary binding tests. The enzymes fell into two groups immunologically, those purified from plasma and pancreas in one and those from spleen and liver in the other. No antigenic cross-reaction between the two groups was detected by any of the immunoassays used. There was a slight antigenic cross-reaction between the human and bovine pancreatic RNAases. The liver and spleen RNAases were immunologically identical by all criteria used, whereas a small but consistent antigenic difference between the human plasma and human pancreas enzymes was detected. The significance of this difference between the human plasma and pancreas RNAases is discussed in relation to similarities and differences in their properties.

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