scholarly journals Observations on Somatic Embryogenesis in Coffea arabica L.

2020 ◽  
Author(s):  
Julieta Andrea Silva de Almeida
Keyword(s):  
Somatic Embryogenesis ◽  
Coffea Arabica ◽  
Coffea Arabica L

scholarly journals The effect of auxin 2,4-D and cytokinin 2-ip on direct somatic embryogenesis formation of Coffea arabica L. leaf explant

2011 ◽  
Vol 27 (2) ◽  
Author(s):  
Rina Arimarsetiowati
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Coffea Arabica ◽  
Callus Formation ◽  
Culture Technique ◽  
Plant Production ◽  
Direct Somatic Embryogenesis ◽  
Arabica Coffee ◽  
Randomized Design ◽  
Coffea Arabica L

One of the propagation technique for coffee plant production is tissue culture. Tissue culture technique for Coffea arabica L. faces some problems, mainly in the planlet formation regenerated from explants. The objective of this experiment was to examine the effect 2,4-D and 2-ip combination on the formation of direct somatic embryogenesis of Coffea arabica L. in leaves explant. Auxin (2,4-D) and cytokinin (2-ip) concentrations of, respectively, 1; 5 µM and 5; 10; 15; 20 were used as treatments. This research was conducted using completely randomized design with 10 replications. Observation to induce somatic embryos was done by quantitatively on number of callus from explant and number of embryogenic callus. Beside that, observation by qualitative descriptive was also done on deve lopment of embryogenesis. The results showed that Arabica coffee leaves explant of AS 2K clones could be induced in all medium combination except 5µM 2,4-D and 20µM 2-ip combination. Arabica coffee leaves explant of S 795, Sigararutang and AS 1 varieties could be induced in all medium combination. The highest frequency of callus formation was found in AS 2K, Sigararutang and AS 1 varieties on medium containing 1µM 2,4-D in combination with 10µM 2-ip, whereas for the S 795 variety on medium containing 5µM 2,4-D in combination with 10µM 2-ip. The highest frequency of embriogenic callus in all Arabica coffee variety could be reached on medium containing 5µM 2,4-D in combination with 15µM 2-ip. Key words : Coffea arabica L., somatic embryogenesis, 2,4-D, 2-ip, tissue culture, leaves, callus embryogenic.

Differential Gene Expression During Somatic Embryogenesis in Coffea arabica L., Revealed by RT-PCR Differential Display

2002 ◽  
Vol 21 (1) ◽  
pp. 043-050 ◽  
Author(s):  
R Rojas-Herrera ◽  
F Quiroz-Figueroa ◽  
M Monforte-González ◽  
L Sánchez-Teyer ◽  
V M Loyola-Vargas
Keyword(s):  
Gene Expression ◽  
Somatic Embryogenesis ◽  
Differential Gene Expression ◽  
Differential Display ◽  
Coffea Arabica ◽  
Rt Pcr ◽  
Differential Gene ◽  
Coffea Arabica L

Possible involvement of an acidic chitinase during direct somatic embryogenesis in Coffea arabica L

2000 ◽  
Vol 28 (5) ◽  
pp. A405-A405
Author(s):  
R. Rojas-Herrera ◽  
M. Monforte-González ◽  
M. Méndez-Zeel ◽  
V. M. Loyola-Vargas
Keyword(s):  
Somatic Embryogenesis ◽  
Coffea Arabica ◽  
Direct Somatic Embryogenesis ◽  
Coffea Arabica L

PROBLEMS OF SOIL ADAPTATION IN PLANTLETS OF COFFEA ARABICA L. OBTAINED VIA SOMATIC EMBRYOGENESIS

1987 ◽  
pp. 315-321 ◽  
Author(s):  
M.L. Guimarães ◽  
M.C. Pimenta ◽  
J. Montezuma-de-Carvalho
Keyword(s):  
Somatic Embryogenesis ◽  
Coffea Arabica ◽  
Coffea Arabica L ◽  
Soil Adaptation

Influence of triacontanol on somatic embryogenesis in Coffea arabica L. and Coffea canephora P. ex Fr.

2004 ◽  
Vol 40 (2) ◽  
pp. 200-203 ◽  
Author(s):  
P. Ciridhar ◽  
E. P. Indu ◽  
G. A. Ravishankar ◽  
A. Chandrasekar
Keyword(s):  
Somatic Embryogenesis ◽  
Coffea Arabica ◽  
Coffea Canephora ◽  
Coffea Arabica L

Validation of reference genes for qPCR analysis of Coffea arabica L. somatic embryogenesis-related tissues

2016 ◽  
Vol 128 (3) ◽  
pp. 663-678 ◽  
Author(s):  
Natália Chagas Freitas ◽  
Horllys Gomes Barreto ◽  
Christiane Noronha Fernandes-Brum ◽  
Rafael Oliveira Moreira ◽  
Antonio Chalfun-Junior ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Reference Genes ◽  
Coffea Arabica ◽  
Qpcr Analysis ◽  
Coffea Arabica L

scholarly journals 2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L.

2018 ◽  
Vol 48 (11) ◽  
Author(s):  
Ivanilda dos Santos Alves ◽  
Valéria Cristina Barbosa Carmazini ◽  
Cosme Damião dos Santos ◽  
Julieta Andrea Silva de Almeida
Keyword(s):  
Somatic Embryogenesis ◽  
Salt Concentration ◽  
Somatic Embryos ◽  
Coffea Arabica ◽  
Leaf Explants ◽  
Direct Somatic Embryogenesis ◽  
Coffea Arabica L

ABSTRACT: This study evaluated the effect of different concentrations of 2-isopentenyladenine (2-iP) on the direct somatic embryogenesis capacity of the Mundo Novo cultivar of Coffea arabica. Leaf explants were cultivated with half the MS salt concentration and the addition of sucrose (20gL-1) and 2-iP (0; 2.5; 5; 7.5 and 10µM). The 2-iP doses of 7.5 and 10µM produced the greatest responses with respect to the percentage of explants with embryogenic structures and the size of the embryogenic structures. However, the greatest production of somatic embryos occurred on the explants treated with 10µM of 2-iP, followed by 7.5µM, whereas their production was absent or reduced with 0 and 5µM, respectively.

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