Differential Gene Expression During Somatic Embryogenesis in Coffea arabica L., Revealed by RT-PCR Differential Display

2002 ◽  
Vol 21 (1) ◽  
pp. 043-050 ◽  
Author(s):  
R Rojas-Herrera ◽  
F Quiroz-Figueroa ◽  
M Monforte-González ◽  
L Sánchez-Teyer ◽  
V M Loyola-Vargas
Keyword(s):  
Gene Expression ◽  
Somatic Embryogenesis ◽  
Differential Gene Expression ◽  
Differential Display ◽  
Coffea Arabica ◽  
Rt Pcr ◽  
Differential Gene ◽  
Coffea Arabica L

scholarly journals Differential Gene Expression in Sugarcane in Response to Challenge by Fungal PathogenUstilago scitamineaRevealed by cDNA-AFLP

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Que You-Xiong ◽  
Lin Jian-Wei ◽  
Song Xian-Xian ◽  
Xu Li-Ping ◽  
Chen Ru-Kai
Keyword(s):  
Gene Expression ◽  
Differential Gene Expression ◽  
Silver Staining ◽  
Molecular Basis ◽  
Pcr Analysis ◽  
Aflp Analysis ◽  
Rt Pcr ◽  
Resistant Genotype ◽  
Ustilago Scitaminea ◽  
Differential Gene

Differential gene expression in sugarcane during sugarcane-Ustilago scitamineainteraction was conducted in a smut-resistant genotype. Using cDNA-AFLP along with silver staining, a total of 136 transcript-derived fragments (TDFs) were found to be differentially expressed in response to challenge byU. scitaminea. Forty TDFs, 34 newly induced plus six with obvious upregulated expression after infection, were sequenced and validated by RT-PCR analysis. These results demonstrated that the expression of 37 out of these TDFs in RT-PCR analysis was consistent with that in cDNA-AFLP analysis. Based on BlastX in NCBI, 28 TDFs were assumed to function in sugarcane underU. scitamineastress. Analysis of expression profile of three TDFs revealed that they responded differently after infection withU. scitaminea, and the transcription was significantly enhanced. The response of two TDFs, SUC06 and SUC09, occurred before that of SUC10. This study enriches our knowledge of the molecular basis for sugarcane response toU. scitamineainfection.

Differential gene expression during somatic embryogenesis in the maize (Zea mays L.) inbred line H99

2011 ◽  
Vol 109 (2) ◽  
pp. 271-286 ◽  
Author(s):  
Lifang Sun ◽  
Ying Wu ◽  
Shengzhong Su ◽  
Hongkui Liu ◽  
Guang Yang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Zea Mays ◽  
Somatic Embryogenesis ◽  
Differential Gene Expression ◽  
Inbred Line ◽  
Zea Mays L ◽  
Differential Gene

Differential Gene Expression in Hemodialysis Patients with "Cold" Zheng

2006 ◽  
Vol 34 (03) ◽  
pp. 377-385 ◽  
Author(s):  
Chia-Sheng Chen ◽  
Li-Wei Lin ◽  
Chia-Chang Hsieh ◽  
Guang-Wei Chen ◽  
Wen-Huang Peng ◽  
...  
Keyword(s):  
Gene Expression ◽  
Differential Gene Expression ◽  
Hemodialysis Patients ◽  
Group Differences ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Lower Serum ◽  
Inflammatory Gene Expression ◽  
Differential Gene ◽  
Polymerase Chain

The aim of the present study was to search for the differential gene expression and measure the serum level of a number of biochemical parameters in the cold Zheng (CZ) and non-cold Zheng (NCZ) in patients receiving hemodialysis. Hemodialysis (HD) patients were randomly selected from the CZ and NCZ groups. The between-group differences in gene expression were assessed using complementary DNA (cDNA) microarray. Differential gene expression was further validated by real-time reverse transcriptase polymerase chain reaction (RT-PCR). Our results demonstrated that the up-regulation of the inflammation-associated genes, ALOX5AP, S100A8 and S100A12, down-regulation of the genes related to immunity (DEFA4), metabolism (GNG11, PYGB, PRKAR2B), and growth/proliferation (HSF2, DDR2, TK1) were found in the CZ group. Furthermore, the CZ HD patients had significantly lower serum albumin levels compared with their NCZ counterparts (3.31 ± 0.08 g/dL versus 4.18 ± 0.12 g/dL ). It appears reasonable to conclude that up-regulated inflammatory-gene expression (ALOX5AP, S100A8 and S100A12) may play an important role in CZ HD patients.

Sign in / Sign up

Export Citation Format

Share Document