scholarly journals The neuroactive neurosteroid Dehydroepiandrosterone Sulfate (DHEAS) modulates the serotonergic system within the dorsal Raphe nucleus and the cerebrospinal fluid release of Reissner’s fiber in rat

2020 ◽  
Vol 343 (1) ◽  
pp. 101-110
Author(s):  
Omar El Hiba ◽  
Ahmed Draoui ◽  
Halima Gamrani
Keyword(s):  
Cerebrospinal Fluid ◽  
Dorsal Raphe Nucleus ◽  
Dehydroepiandrosterone Sulfate ◽  
Dorsal Raphe ◽  
Raphe Nucleus ◽  
Serotonergic System ◽  
Reissner's Fiber ◽  
Reissner’S Fiber ◽  
Fluid Release ◽  
Dorsal Raphé

scholarly journals Serotonergic plasticity in the dorsal raphe nucleus characterizes susceptibility and resilience to anhedonia

2019 ◽  
Author(s):  
Nandkishore Prakash ◽  
Christiana J. Stark ◽  
Maria N. Keisler ◽  
Lily Luo ◽  
Andre Der-Avakian ◽  
...  
Keyword(s):  
Chronic Stress ◽  
Dorsal Raphe Nucleus ◽  
Dorsal Raphe ◽  
Raphe Nucleus ◽  
Serotonergic System ◽  
Male Rats ◽  
Vulnerability To Stress ◽  
Neurotransmitter Plasticity ◽  
New Treatments ◽  
Dorsal Raphé

ABSTRACTChronic stress induces anhedonia in susceptible, but not resilient individuals, a phenomenon observed in humans as well as animal models, but the molecular mechanisms underlying susceptibility and resilience are not well understood. We hypothesized that the serotonergic system, which is implicated in stress, reward and antidepressant therapy, may play a role. We found that plasticity of the serotonergic system contributes to the differential vulnerability to stress displayed by susceptible and resilient animals. Stress-induced anhedonia was assessed in adult male rats using social defeat and intracranial self-stimulation (ICSS), while changes in serotonergic phenotype were investigated using immunohistochemistry and in situ hybridization. Susceptible, but not resilient, rats displayed an increased number of neurons expressing the biosynthetic enzyme for serotonin, tryptophan-hydroxylase-2 (TPH2), in the ventral subnucleus of the dorsal raphe nucleus (DRv). Further, a decrease in the number of DRv glutamatergic neurons was observed in all stressed animals. This neurotransmitter plasticity is dependent on DR activity, as was revealed by chemogenetic manipulation of the central amygdala, a stress-sensitive nucleus that forms a major input to the DR. Activation of amygdalar corticotropin releasing hormone (CRH)+ neurons abolished the increase in DRv TPH2+ neurons and ameliorated stress-induced anhedonia in susceptible animals. These findings show that activation of amygdalar projections induces resilience, and suppresses the gain of serotonergic phenotype in the DR that is characteristic of susceptible animals. This molecular signature of vulnerability to stress-induced anhedonia and the active nature of resilience could be a target of new treatments for stress-related disorders like depression.SIGNIFICANCE STATEMENTDepression and other mental disorders can be induced by chronic or traumatic stressors. However, some individuals are resilient and do not develop depression in response to chronic stress. A complete picture of the molecular differences between susceptible and resilient individuals is necessary to understand how plasticity of limbic circuits is associated with the pathophysiology of stress-related disorders. Using a rodent model, our study identifies a novel molecular marker of susceptibility to stress-induced anhedonia, a core symptom of depression, and a means to modulate it. These findings will guide further investigation into cellular and circuit mechanisms of resilience, and the development of new treatments for depression.

scholarly journals Ginsenoside Rg1 promotes sleep in rats by modulating the noradrenergic system in the locus coeruleus and serotonergic system in the dorsal raphe nucleus

2019 ◽  
Vol 116 ◽  
pp. 109009 ◽  
Author(s):  
Ya-Ping Xu ◽  
Xiang-Yu Cui ◽  
Yu-Tong Liu ◽  
Su-Ying Cui ◽  
Yong-He Zhang
Keyword(s):  
Locus Coeruleus ◽  
Dorsal Raphe Nucleus ◽  
Dorsal Raphe ◽  
Raphe Nucleus ◽  
Serotonergic System ◽  
Noradrenergic System ◽  
Ginsenoside Rg1 ◽  
Dorsal Raphé

Author response for "The Mouse Dorsal Raphe Nucleus as Understood by Temporal Fgf8 Lineage Analysis"

2020 ◽  
Author(s):  
Herminio M. Guajardo ◽  
Paul G. Hatini ◽  
Kathryn G. Commons
Keyword(s):  
Dorsal Raphe Nucleus ◽  
Dorsal Raphe ◽  
Raphe Nucleus ◽  
Author Response ◽  
Dorsal Raphé ◽  
Lineage Analysis

Buspirone-induced changes in the serotonergic and non-serotonergic cells in the dorsal raphe nucleus of rats

2010 ◽  
Vol 473 (2) ◽  
pp. 136-140 ◽  
Author(s):  
Ali Jahanshahi ◽  
Lee Wei Lim ◽  
Harry W.M. Steinbusch ◽  
Veerle Visser-Vandewalle ◽  
Yasin Temel
Keyword(s):  
Dorsal Raphe Nucleus ◽  
Dorsal Raphe ◽  
Raphe Nucleus ◽  
Induced Changes ◽  
Dorsal Raphé

027 GABAergic Neurons in the Dorsal Raphe Nucleus Are under the Influence of GABAergic Inputs from the Nucleus Pontis Oralis

SLEEP ◽  
2021 ◽  
Vol 44 (Supplement_2) ◽  
pp. A12-A12
Author(s):  
Jianhua Zhang ◽  
Mingchu Xi ◽  
Simon Fung ◽  
Charles Tobin ◽  
Sharon Sampogna ◽  
...  
Keyword(s):  
Dorsal Raphe Nucleus ◽  
Dorsal Raphe ◽  
Gabaergic Neurons ◽  
Fluorescent Labeling ◽  
Raphe Nucleus ◽  
Gaba A ◽  
Adult Cats ◽  
Immunohistochemical Techniques ◽  
Fluorescence Immunohistochemistry ◽  
Dorsal Raphé

Abstract Introduction Our previous study has shown that there is a direct connection between GABAergic neurons in the nucleus pontis oralis (NPO) and neurons of the dorsal raphe nucleus (DR), providing a morphological basis for the hypothesis that GABAergic inhibitory processes in NPO play an important role in the generation and maintenance of wakefulness as well as active (REM) sleep through the interaction with neurons in the DR. However, the target of such a GABAergic projection from the NPO within the DR is unknown. In the present study, a double-fluorescent labeling technique was employed to examine the target of GABAergic inputs to the DR. Methods Adult cats were deeply anesthetized and perfused transcardially. Subsequently, the brainstem containing the DR was removed, postfixed and cut into 15 μm coronal sections with a Reichert-Jung cryostat. The sections were immunostained with antibodies against GABA-A or GABA-B receptors and GABA following the procedure of double fluorescence immunohistochemistry. Results Under fluorescence microscopy, a large number of neurons were labeled with antibodies against either GABA-A receptor or GABA-B receptor. In addition, neurons labeled with antibody against GABA were observed in the DR. With double fluorescence immunohistochemical techniques, some neurons labeled by anti-GABA antibody were also stained with antibodies against GABA-A or GABA-B receptors. Conclusion The expression of GABA-A or GABA-B receptors by GABAergic neurons in the DR indicates that GABAergic neurons in the DR receive GABAergic inputs. Our previous study has demonstrated that these GABAergic inputs are from the NPO. These data provide a morphological foundation to support our hypothesis that, during wakefulness, NPO GABAergic “Executive” neurons suppress “Second-Order” GABAergic neurons in the DR, which, in turn, activate (disinhibit) serotonergic wake-on neurons in this nucleus. Support (if any) NS092383

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