A Study of Topical Application-Revaluation of Application Methods. II.

The novel ingestion bioassay method was developed for detecting and monitoring resistance of Euschistus heros by encapsulating an artificial liquid diet using Parafilm®. This methodology was compared with the tarsal contact (vial test) and topical application methods for thiamethoxam, imidacloprid, and lambda-cyhalothrin. The best bioassay method for the neonicotinoid insecticides thiamethoxam and imidacloprid was ingestion. For pyrethroid insecticide lambda-cyhalothrin, the best result was obtained by topical application. Using the best bioassay method for each insecticide, the susceptibility to these insecticides was monitored in 30 populations of E. heros collected from soybean crops in Brazil from 2018 to 2020. High variations in susceptibility to thiamethoxam (resistance ratios, 1.6–22 times), imidacloprid (resistance ratios, 1.6–22 times), and lambda-cyhalothrin (resistance ratios, 5–40 times) were detected among the evaluated E. heros populations. In order to monitor the susceptibility of E. heros to insecticides, diagnostic concentrations were defined based on the LC99 of the susceptible reference population: 5.65 µL of a.i./mL for thiamethoxam, 12.45 µL of a.i./mL for imidacloprid, and 0.20 µg of a.i./insect for lambda-cyhalothrin. Subsequently, we select an E. heros strain resistant to neonicotinoid insecticides and another to lambda-cyhalothrin. The resistance ratios obtained after seven selection cycles were 66, 41 and 44 times for thiamethoxam, imidacloprid and lambda-cyhalothrin, respectively.

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Effect of buprofezin on the growth, development and morphometric parameters of Spodoptera litura (Fabricius) (Order: Lepidoptera, Family: Noctuidae) adult

Journal of the Bangladesh Agricultural University ◽

10.3329/jbau.v17i4.44620 ◽

2019 ◽

Vol 17 (4) ◽

pp. 514-520

Author(s):

Mst Rokeya Khatun ◽

Gopal Das ◽

Kazi Shahanara Ahmed ◽

Hisashi Kato- Noguchi

Keyword(s):

Relative Humidity ◽

Topical Application ◽

Growth Regulator ◽

Growth And Development ◽

Spodoptera Litura ◽

Wing Length ◽

Adult Weight ◽

Application Method ◽

Combined Application ◽

Application Methods

The experiments were conducted to know the efficacy of Buprofezin (Award 40 SC), an insect growth regulator against 2nd instar larvae of Spodoptera litura (Fabricius) under laboratory conditions (insect rearing was done at room temperature and relative humidity that is 260C-300C and 65-80% relative humidity) at three different concentrations viz. 200, 400, and 600 ppm through three different application methods like direct or topical, indirect or leaf-dip and combined. The experiment was laid out in complete randomized design (CRD) with three replications and ten 2nd instar larvae of Spodoptera litura were used for each replication. Results revealed that this growth regulator strongly arrested the growth and development of S. litura (Fabricius) from larval stage to adult. The treated data were recorded after adult emergence and compared with untreated control adult. The results clearly showed that Buprofezin had significant effect on the inhibition of adult weight; wing length and width compared to control and consequence were clearly dose, time and method dependent. As well as we are hopeful because we found some abnormal adult from the treated larvae that may increase the potentiality of Buprofezin as an excellent tool of IPM. The maximum, 54.53% adult weight reduction was recorded from 600 ppm of Buprofezin through combined application method which was followed by leaf-dip (46.41%) and topical application method (39.86%). Similarly, 23.13% wing length and 22.27% wing width were inhibited when larvae were treated with 600 ppm through combined application method compared to control. The 2nd best result was found from 400 ppm and leaf-dip application method. The dose, 200 ppm and topical application method was found to be less effective compared to the rest of the two doses or two application methods. So this is the demand of the current situation to evaluate this selective bio-rational molecules against S. litura as the alternatives of conventional insecticides to combat resistance development, effective larval control, inhibition of growth and development, to keep the natural enemies safe and fit these molecules in IPM packages individually or combindly. J Bangladesh Agril Univ 17(4): 514–520, 2019

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A Study on Topical Application. Revaluation of Application Methods. I.

JOURNAL OF DENTAL HEALTH ◽

10.5834/jdh.44.97 ◽

1994 ◽

Vol 44 (1) ◽

pp. 97-103

Author(s):

Akiko NISHIDA ◽

Mizuo KANI ◽

Shunji IKUTA ◽

Kenji YOKOI ◽

Atsunori ISOZAKI ◽

...

Keyword(s):

Topical Application ◽

Application Methods

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Ultrastructure of Mouse Basal Cell Carcinoma Exhibiting Sebaceous Differentiation

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s1431927600003214 ◽

1980 ◽

Vol 38 ◽

pp. 738-739

Author(s):

Victoria L. Wade ◽

Winslow G. Sheldon ◽

James W. Townsend ◽

William Allaben

Keyword(s):

Basal Cell Carcinoma ◽

Cell Carcinoma ◽

Topical Application ◽

Basal Cell ◽

Sebaceous Gland ◽

Rats And Mice ◽

Mixed Tumors

Sebaceous gland tumors and other tumors exhibiting sebaceous differentiation have been described in humans (1,2,3). Tumors of the sebaceous gland can be induced in rats and mice following topical application of carcinogens (4), but spontaneous mixed tumors of basal cell origin rarely occur in mice.

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