Histopathological alterations kidney in adult male rats that prenatally exposed to diclofenac sodium: A histopathological study

The aim of the present study was to compare the response to acute application of several drugs in adult male and female rats prenatally exposed to methamphetamine (MA). Spontaneous locomotor activity and exploratory behavior of adult male and female rats prenatally exposed to MA (5 mg/kg) or saline were tested in a Laboras apparatus (Metris B.V., Netherlands) for 1 h. Challenge dose of the examined drug [amphetamine – 5 mg/kg; cocaine – 5mg/kg; MDMA (3,4-methylenedioxymethamphetamine) – 5 mg/kg; morphine – 5 mg/kg; THC (delta9-tetrahydrocannabinol) – 2 mg/kg] or saline was injected prior to testing. Our data demonstrate that prenatal MA exposure did not affect behavior in male rats with cocaine or morphine treatment, but increased locomotion and exploration in females. Application of amphetamine and MDMA in adulthood increased activity in both sexes, while cocaine and THC only in female rats. Morphine, on the other hand, decreased the activity in the Laboras test in both sexes. As far as sex and estrous cycle is concerned, the present study shows that males were generally less active than females and also females in proestrus-estrus phase of the estrous cycle were more active than females in diestrus. In conclusion, the present study shows that the prenatal MA exposure does not induce general sensitization but affects the sensitivity to drugs dependently to mechanism of drug action and with respect to gonadal hormones.

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Hypothalamic-pituitary-adrenocortical and gonadal axes and sympathoadrenal activity of adult male rats prenatally exposed to morphine

Neuroscience Letters ◽

10.1016/s0304-3940(99)00086-5 ◽

1999 ◽

Vol 263 (1) ◽

pp. 1-4 ◽

Cited By ~ 11

Author(s):

Isabelle Dutriez-Casteloot ◽

François Bernet ◽

Jean-François Dedieu ◽

Dominique Croix ◽

Christine Laborie ◽

...

Keyword(s):

Adult Male ◽

Male Rats ◽

Prenatally Exposed ◽

Sympathoadrenal Activity

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Can Anxiety Tested in the Elevated Plus-maze Be Related to Nociception Sensitivity in Adult Male Rats?

Prague Medical Report ◽

10.14712/23362936.2016.19 ◽

2016 ◽

Vol 117 (4) ◽

pp. 185-197 ◽

Cited By ~ 3

Author(s):

Marie Pometlová ◽

Anna Yamamotová ◽

Kateryna Nohejlová ◽

Romana Šlamberová

Keyword(s):

Adult Male ◽

Elevated Plus Maze ◽

Male Rats ◽

Acute Administration ◽

Prenatally Exposed ◽

Pregnant Rats ◽

Anxiogenic Effect ◽

Plus Maze ◽

Plantar Test ◽

High Responders

Methamphetamine (MA) is one of the most addictive psychostimulant drugs with a high potential for abuse. Our previous studies demonstrated that MA administered to pregnant rats increases pain sensitivity and anxiety in their adult offspring and makes them more sensitive to acute administration of the same drug in adulthood. Because individuals can differ considerably in terms of behaviour and physiology, such as rats that do not belong in some characteristics (e.g. anxiety) to average, can be described as low-responders or high-responders, are then more or less sensitive to pain. Therefore, prenatally MA-exposed adult male rats treated in adulthood with a single dose of MA (1 mg/ml/kg) or saline (1 ml/kg) were tested in the present study. We examined the effect of acute MA treatment on: (1) the anxiety in the Elevated plus-maze (EPM) test and memory in EPM re-test; (2) nociception sensitivity in the Plantar test; (3) the correlation between the anxiety, memory and the nociception. Our results demonstrate that: (1) MA has an anxiogenic effect on animals prenatally exposed to the same drug in the EPM; (2) all the differences induced by acute MA treatment disappeared within the time of 48 hours; (3) there was no effect of MA on nociception per se, but MA induced higher anxiety in individuals less sensitive to pain than in animals more sensitive to pain. In conclusion, the present study demonstrates unique data showing association between anxiety and nociceptive sensitivity of prenatally MA-exposed rats that is induced by acute drug administration.

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Cognitive functions and drug sensitivity in adult male rats prenatally exposed to methamphetamine

Physiological Research ◽

10.33549/physiolres.931562 ◽

2009 ◽

pp. 741-750

Author(s):

B Schutová ◽

L Hrubá ◽

M Pometlová ◽

K Deykun ◽

R Šlamberová

Keyword(s):

Morris Water Maze ◽

Adult Male ◽

Cognitive Functions ◽

Water Maze ◽

Memory Test ◽

Male Rats ◽

Prenatally Exposed ◽

Place Navigation ◽

Probe Test ◽

Navigation Test

The aim of the present study was to investigate the impact of prenatal methamphetamine (MA) exposure and application of the same drug in adulthood on cognitive functions of adult male rats tested in Morris water maze (MWM). Adult male rats prenatally exposed to MA (5 mg/kg), saline or no injection were examined. Half of the animals were injected daily with MA (1 mg/kg) after finishing the testing. Three types of tests were used: (1) “Place navigation test” (Learning), (2) “Probe test” and (3) “Retention memory test” (Memory). Our results showed that prenatal MA exposure did not affect the test of learning and the Probe test. In the test of memory prenatally MA-exposed rats showed smaller search errors and used spatial strategies more than both control groups. Further, MA application in adulthood prolonged trajectories, increased the incidence of random search and decreased the incidence of direct swim in the Place navigation test. In addition, MA administration in adulthood increased the speed of swimming regardless of prenatal exposure. The present study thus demonstrates that 1) Prenatal MA exposure does not affect learning in the MWM, 2) Prenatal MA exposure improves performance in the Retention memory test in the MWM, and 3) MA application in adulthood impairs learning in the Morris water maze.

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