Cognitive functions and drug sensitivity in adult male rats prenatally exposed to methamphetamine

The aim of the present study was to investigate the impact of prenatal methamphetamine (MA) exposure and application of the same drug in adulthood on cognitive functions of adult male rats tested in Morris water maze (MWM). Adult male rats prenatally exposed to MA (5 mg/kg), saline or no injection were examined. Half of the animals were injected daily with MA (1 mg/kg) after finishing the testing. Three types of tests were used: (1) “Place navigation test” (Learning), (2) “Probe test” and (3) “Retention memory test” (Memory). Our results showed that prenatal MA exposure did not affect the test of learning and the Probe test. In the test of memory prenatally MA-exposed rats showed smaller search errors and used spatial strategies more than both control groups. Further, MA application in adulthood prolonged trajectories, increased the incidence of random search and decreased the incidence of direct swim in the Place navigation test. In addition, MA administration in adulthood increased the speed of swimming regardless of prenatal exposure. The present study thus demonstrates that 1) Prenatal MA exposure does not affect learning in the MWM, 2) Prenatal MA exposure improves performance in the Retention memory test in the MWM, and 3) MA application in adulthood impairs learning in the Morris water maze.

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The study of spatial memory in adult male rats with injection of testosterone enanthate and flutamide into the basolateral nucleus of the amygdala in Morris water maze

Brain Research ◽

10.1016/s0006-8993(03)02227-3 ◽

2003 ◽

Vol 972 (1-2) ◽

pp. 1-8 ◽

Cited By ~ 40

Author(s):

Nasser Naghdi ◽

Shahrbanoo Oryan ◽

Roshanak Etemadi

Keyword(s):

Spatial Memory ◽

Morris Water Maze ◽

Adult Male ◽

Water Maze ◽

Male Rats ◽

Testosterone Enanthate ◽

Basolateral Nucleus

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3α-diol administration decreases hippocampal PKA (II) mRNA expression and impairs Morris water maze performance in adult male rats

Behavioural Brain Research ◽

10.1016/j.bbr.2014.11.038 ◽

2015 ◽

Vol 280 ◽

pp. 149-159 ◽

Cited By ~ 11

Author(s):

Somayeh Assadian Narenji ◽

Nasser Naghdi ◽

Kayhan Azadmanesh ◽

Rosita Edalat

Keyword(s):

Mrna Expression ◽

Morris Water Maze ◽

Adult Male ◽

Water Maze ◽

Male Rats ◽

Maze Performance

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Effect of Methamphetamine on Cognitive Functions of Adult Female Rats Prenatally Exposed to the Same Drug

Physiological Research ◽

10.33549/physiolres.932598 ◽

2013 ◽

pp. S89-S98 ◽

Cited By ~ 3

Author(s):

E. MACÚCHOVÁ ◽

K. NOHEJLOVÁ-DEYKUN ◽

R. ŠLAMBEROVÁ

Keyword(s):

Estrous Cycle ◽

Prenatal Exposure ◽

Adult Female ◽

Cognitive Functions ◽

Female Rats ◽

Saline Control ◽

Control Group ◽

Prenatally Exposed ◽

Place Navigation ◽

Navigation Test

The aim of this study was to investigate the effect of prenatal methamphetamine (MA) exposure and application of the same drug in adulthood on cognitive functions of adult female rats. Animals were prenatally exposed to MA (5 mg/kg) or saline (control group). The cognitive function was tested as ability of spatial learning in the Morris Water Maze (MWM). Each day of the experiment animals received an injection of MA (1 mg/kg) or saline. Our results demonstrated that prenatal MA exposure did not affect the latency to reach the hidden platform or the distance traveled during the Place Navigation Test; however, the speed of swimming was increased in prenatally MA-exposed rats compared to controls regardless of the treatment in adulthood. MA treatment in adulthood increased the latency and distance when compared to controls regardless of the prenatal exposure. Neither prenatal exposure, nor treatment in adulthood affected memory retrieval. As far as the estrous cycle is concerned, our results showed that prenatally MA-exposed females in proestrus/estrus swam faster than females in diestrus. This effect of estrous cycle was not apparent in control females. In conclusion, our results indicate that postnatal, but not prenatal exposure to MA affects learning of adult female rats.

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Exposure to Methamphetamine During First and Second Half of Prenatal Period and Its Consequences on Cognition After Long-Term Application in Adulthood

Physiological Research ◽

10.33549/physiolres.932927 ◽

2014 ◽

pp. S535-S545 ◽

Cited By ~ 1

Author(s):

I. HREBÍČKOVÁ ◽

M. MALINOVÁ-ŠEVČÍKOVÁ ◽

E. MACÚCHOVÁ ◽

K. NOHEJLOVÁ ◽

R. ŠLAMBEROVÁ

Keyword(s):

Male Rats ◽

Daily Injection ◽

Memory Retention ◽

Critical Periods ◽

Cognitive Changes ◽

Intrauterine Development ◽

Place Navigation ◽

Probe Test ◽

Navigation Test

It is known that psychostimulants including methamphetamine (MA) have neurotoxic effect, especially, if they are targeting CNS during its critical periods of development. The present study was aimed on evaluation of cognitive changes following scheduled prenatal MA exposure in combination with long-term exposure in adulthood of male rats. Two periods of gestation were targeted: 1st half – the embryonic day (ED) 1-11 and 2nd half – ED 12-22. Rat mothers received subcutaneously a daily injection of MA (5 mg/kg) or saline (SAL, 1 ml/kg) throughout scheduled periods. Male offspring were tested for cognitive changes in the Morris Water Maze (MWM) in adulthood. Each day of the experiment animals received an injection of MA (1 mg/kg) or SAL (1 ml/kg) during 12 days. Our results demonstrated that in the group of animals exposed to the drug during ED 1-11, neither prenatal MA exposure, nor adult MA treatment changed the performance in the MWM test. Only the velocity was increased in group with long-term MA treatment (SAL/MA and MA/MA). In the group of animals exposed to the drug during ED 12-22, rats exposed to MA prenatally and also in adulthood (MA/MA) swam faster but learned the position of the platform slower in the Place Navigation Test than animals exposed to SAL in adulthood (MA/SAL). In the Probe Test, MA/SAL had decreased velocity and swam shorter distance than MA/MA or SAL/SAL rats suggesting increased floating of these animals. In the Memory Retention Test, SAL/MA rats swam shorter distance than SAL/SAL or MA/MA animals suggesting changes in used strategies in memory recall. As conclusion, our results suggest differences in the effect of combination of prenatal and adult exposure to MA. These effects further depend on the stage of CNS development and schedule of MA exposure affecting intrauterine development in male rats.

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Beneficial effect of retigabine on memory in rats receiving ethanol

Pharmacological Reports ◽

10.1007/s43440-020-00205-z ◽

2020 ◽

Author(s):

Ewa Zwierzyńska ◽

Agata Krupa-Burtnik ◽

Bogusława Pietrzak

Keyword(s):

Beneficial Effect ◽

Fear Conditioning ◽

Morris Water Maze ◽

Water Maze ◽

Experimental Models ◽

Male Rats ◽

Ethanol Administration ◽

Free Access ◽

Oral Gavage ◽

The Impact

Abstract Background Retigabine belongs to the novel generation of antiepileptic drugs but its complex mechanism of action causes that the drug might be effective in other diseases, for instance, alcohol dependence. It is known that ethanol abuse impaired the function of brain structures associated with memory and learning such as the hippocampus. In our previous study, retigabine reduced hippocampal changes induced by ethanol in the EEG rhythms in rabbits. This study is focused on the impact of retigabine on memory processes in male rats receiving alcohol. Methods Memory was evaluated in various experimental models: Morris water maze, Contextual, and Cued Fear Conditioning tests. Retigabine was administered for 3 weeks directly to the stomach via oral gavage at a dose of 10 mg/kg. Rats received also 20% ethanol (5 g/kg/day in two doses) via oral gavage for 3 weeks and had free access to 5% ethanol in the afternoon and at night. Morris water maze was performed after 1 and 3 weeks of ethanol administration and after 1 week from the discontinuation of ethanol administration. Contextual and Cued Fear Conditioning tests were carried out after 24 h and 72 h of alcohol discontinuation. Results The drug significantly decreased ethanol-induced memory disturbances during alcohol administration as well as slightly improved learning processes after the discontinuation of ethanol administration. Conclusions This beneficial effect of retigabine-ethanol interaction on memory may be a relevant element of the drug’s impact on the development of addiction.

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