Description of a new species of Loxosceles Heineken & Lowe (Araneae, Sicariidae) recluse spiders from Hidalgo, Mexico, under integrative taxonomy: morphological and DNA barcoding data (CO1 + ITS2)

Integrative Taxonomy ◽

Taxonomic Approach ◽

Unique Species ◽

Based on an integrative taxonomic approach, a new species of the genus Loxosceles Heineken & Lowe, 1832, is described from the state of Hidalgo, Mexico. Loxosceles tolantongo sp. nov. is described based on DNA barcoding using cytochrome c oxidase subunit 1 (CO1) and internal transcribed spacer 2 (ITS2), and morphology. For species delimitation, four molecular methods were implemented: 1) corrected p-distances under neighbor joining (NJ); 2) automatic barcode gap discovery (ABGD); 3) general mixed yule coalescent model (GMYC) and 4) Bayesian Poisson tree processes (bPTP). The new species morphologically resembles L. jaca, another species from Hidalgo, but there are morphological differences mainly in the tibiae of the male palp, the seminal receptacles of the females and also the high genetic p-distances. CO1 was more informative than ITS2 for the genetic separation; however, both concatenated genes (CO1 + ITS2) present robust evidence for species delimitation. Loxosceles tolantongo sp. nov. is considered a unique species for four reasons: 1) it can be diagnosed and distinguished by morphological characters (of the male palps mainly, but also of the seminal receptacles of the females); 2) the genetic p-distances with CO1 were high (>10%); 3) the molecular species delimitation methods were congruent under CO1 and CO1 + ITS2; and 4) under CO1 and CO1 + ITS2, the new species is a putative sister group of L. jaca + L. tenango.

Under an integrative taxonomic approach: the description of a new species of the genus Loxosceles (Araneae, Sicariidae) from Mexico City

ZooKeys ◽

10.3897/zookeys.892.39558 ◽

2019 ◽

Vol 892 ◽

pp. 93-133 ◽

Cited By ~ 4

Author(s):

Alejandro Valdez-Mondragón ◽

Claudia I. Navarro-Rodríguez ◽

Karen P. Solís-Catalán ◽

Mayra R. Cortez-Roldán ◽

Alma R. Juárez-Sánchez

Keyword(s):

New Species ◽

Mexico City ◽

Molecular Species ◽

Species Delimitation ◽

Molecular Data ◽

Integrative Taxonomy ◽

Taxonomic Approach ◽

Unique Species ◽

A new species of the spider genus Loxosceles Heineken & Lowe, 1832, Loxosceles tenochtitlan Valdez-Mondragón & Navarro-Rodríguez, sp. nov., is described based on adult male and female specimens from the states of Mexico City, Estado de Mexico and Tlaxcala. Integrative taxonomy including traditional morphology, geometric and lineal morphology, and molecules (DNA barcodes of cytochrome c oxidase subunit 1 (CO1) and internal transcribed spacer 2 (ITS2)), were used as evidence to delimit the new species. Four methods were used for molecular analyses and species delimitation: 1) corrected p-distances under neighbor joining (NJ), 2) automatic barcode gap discovery (ABGD), 3) general mixed yule coalescent model (GMYC), and 4) poisson tree processes (bPTP). All molecular methods, traditional, geometric and lineal morphology were consistent in delimiting and recognizing the new species. Loxosceles tenochtitlansp. nov. is closely related to L. misteca based on molecular data. Although both species are morphologically similar, the average p-distance from CO1 data was 13.8% and 4.2% for ITS2 data. The molecular species delimitation methods recovered well-supported monophyletic clusters for samples of L. tenochtitlansp. nov. from Mexico City + Tlaxcala and for samples of L. misteca from Guerrero. Loxosceles tenochtitlansp. nov. is considered a unique species for three reasons: (1) it can be distinguished by morphological characters (genitalic and somatic); (2) the four different molecular species delimitation methods were congruent to separate both species; and (3) there is variation in leg I length of males between both species, with the males of L. misteca having longer legs than males of L. tenochtitlansp. nov., also morphometrically, the shape of tibiae of the palp between males of both species is different.

Integrative taxonomy of the rock-dwelling gecko Cnemaspis siamensis complex (Squamata, Gekkonidae) reveals a new species from Nakhon Si Thammarat Province, southern Thailand

ZooKeys ◽

10.3897/zookeys.932.50602 ◽

2020 ◽

Vol 932 ◽

pp. 129-159

Author(s):

Natee Ampai ◽

Perry L. Wood Jr ◽

Bryan L. Stuart ◽

Anchalee Aowphol

Keyword(s):

New Species ◽

Integrative Taxonomy ◽

Morphological Data ◽

Mountain Range ◽

Adult Males ◽

Nadh Dehydrogenase Subunit 2 ◽

Southern Thailand ◽

Taxonomic Approach ◽

The rock-dwelling gecko genus Cnemaspis is one of the most species-diverse genera of gekkonid in Thailand. Earlier studies relied on morphological data to identify species, but cryptic morphology often obscured species diversity in Cnemaspis. In this study, an integrative taxonomic approach based on morphological characters and sequences of the mitochondrial NADH dehydrogenase subunit 2 (ND2) gene were used to clarify current taxonomy of the Cnemaspis siamensis complex and delimit a new species from Lan Saka District, Nakhon Si Thammarat Province, southern Thailand. Cnemaspis lineatubercularissp. nov. is distinguished from other congeneric species by the combination of morphological characters: (1) maximum snout-vent length (SVL) of 40.6 mm (mean 38.8 ± SD 1.4, N = 12) in adult males and maximum SVL of 41.8 mm (mean 39.5 ± SD 1.9, N = 7) in adult females; (2) 8–9 supralabial and infralabial scales; (3) gular, pectoral, abdominal, and subcaudal scales keeled; (4) rostral, interorbitals, supercilium, palmar scales, and ventral scales of brachia smooth; (5) 5–6 small, subconical spine-like tubercles present on flanks; (6) 19–21 paravertebral tubercles linearly arranged; (7) 27–29 subdigital lamellae under the fourth toe; (8) 4–7 pore-bearing precloacal scales, pores rounded arranged in chevron shape and separated only in males; (9) one postcloacal tubercles each side in males; (10) ventrolateral caudal tubercles present anteriorly; (11) caudal tubercles restricted to a single paravertebral row on each side; (12) single median row of subcaudal scales keeled and lacking enlarged median row; and (13) gular region, abdomen, limbs and subcaudal region yellowish only in males. Genetically, the uncorrected pairwise divergences between the new species and their congeners in the C. siamensis group were between 15.53–28.09%. The new species is currently known only from granitic rocky streams at Wang Mai Pak Waterfall in the Nakhon Si Thammarat mountain range. Its discovery suggests that additional unrecognized species of Cnemaspis may still occur in unexplored areas of southern Thailand.

Integrative taxonomy of Mexican cycads: biogeography, morphology and DNA barcoding corroborate a new sympatric species in Ceratozamia (Zamiaceae)

Phytotaxa ◽

10.11646/phytotaxa.268.1.2 ◽

2016 ◽

Vol 268 (1) ◽

pp. 25 ◽

Cited By ~ 4

Author(s):

LILÍ MARTÍNEZ-DOMÍNGUEZ ◽

FERNANDO NICOLALDE-MOREJÓN ◽

FRANCISCO VERGARA-SILVA ◽

DENNIS W. STEVENSON

Keyword(s):

New Species ◽

Dna Barcoding ◽

Taxonomic Status ◽

Sympatric Species ◽

Integrative Taxonomy ◽

Los Tuxtlas ◽

A new species, from the montane region of Los Tuxtlas (state of Veracruz, Mexico), Ceratozamia subroseophylla, is proposed in accordance with the integrative taxonomy framework and through application of the taxonomic circle rationale. Our identification of this new species is the result of a thorough re-evaluation of four formerly described Ceratozamia species, namely: (1) C. miqueliana, with which it overlaps geographically; (2) C. mexicana; (3) C. robusta, which has also been previously seen as morphologically similar to C. miqueliana; and (4) C. brevifrons, which was at the center of a controversy concerning the morphological and geographic limits when compared to C. mexicana. Even though there are morphometric affinities of C. subroseophylla with this set of species, there are apomorphies in qualitative morphological characters and diagnostic nucleotidic variants established through character-based DNA barcoding to allow the recognition of this new species. The implications that our integrative taxonomy-based assessment of systematic diversity in Ceratozamia species from Veracruz have for evaluating the overall taxonomic status of the genus are discussed.

A new species of Scrophularia (Scrophulariaceae) from Hubei, China

Phytotaxa ◽

10.11646/phytotaxa.350.1.1 ◽

2018 ◽

Vol 350 (1) ◽

pp. 1

Author(s):

RUI-HONG WANG ◽

MAO-QIN XIA ◽

JIN-BO TAN ◽

CHUAN CHEN ◽

XIN-JIE JIN ◽

...

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Species Delimitation ◽

Central China ◽

Molecular Phylogenetic Analysis ◽

Leaf Margin ◽

Close Relationship ◽

Molecular Phylogenetic ◽

A new species, Scrophularia jinii (Scrophulariaceae), from Central China is described and illustrated. This new species was formerly misidentified as S. fargesii, from which it differs in many morphological characters. Moreover, it is distinct with all known Scrophularia species in its unique deeply double serrate leaf margin with 3–7 big teeth on each side. Molecular phylogenetic analysis further supports its species delimitation and suggests a close relationship with several Japanese and North American species.

Muscari tauricum (Asparagaceae, Scilloideae), a new species from Turkey

Phytotaxa ◽

10.11646/phytotaxa.399.2.1 ◽

2019 ◽

Vol 399 (2) ◽

pp. 109 ◽

Cited By ~ 2

Author(s):

SERPİL DEMİRCİ KAYIRAN ◽

NERİMAN ÖZHATAY ◽

ERDAL KAYA

Keyword(s):

New Species ◽

Flowering Time ◽

Related Species ◽

Early Flowering ◽

Morphological Description ◽

Southern Turkey ◽

In this paper, a new species, Muscari tauricum sp. nov. is described and illustrated from Southern Turkey. It has similar morphological characters to M. aucheri and M. bourgaei except for its broad and several leaves, pyramidal raceme, patent and longer pedicels, small fertile flowers, and early flowering time that distinguish the new species from other Muscari species. A complete morphological description and illustration are given, including comments on karyotypes. The morphological differences among the new species and related species are also discussed.

A new species of the genus Centruroides Marx (Scorpiones, Buthidae) from western Michoacán State, México using molecular and morphological evidence

ZooKeys ◽

10.3897/zookeys.859.33069 ◽

2019 ◽

Vol 859 ◽

pp. 31-48

Author(s):

Ana F. Quijano-Ravell ◽

Luis F. de Armas ◽

Oscar F. Francke ◽

Javier Ponce-Saavedra

Keyword(s):

New Species ◽

Mitochondrial Gene ◽

Integrative Taxonomy ◽

Valid Species ◽

Morphological Evidence ◽

Molecular Evidence ◽

Mountain Range ◽

A New Species ◽

Gene Mrna

A new species of scorpion belonging to the genus Centruroides Marx, 1890 is described from the Coalcomán mountain range, western Michoacán State, Mexico. Its general aspect resembles Centruroidesruana Quijano-Ravell & Ponce-Saavedra, 2016, and C.infamatus (C. L. Koch, 1844), but it is a smaller species having lower pectinal tooth counts; also, males of C.ruana have the pedipalp chelae slightly thicker, whereas C.infamatus has a subaculear tubercle nearer to the base of the aculeus. Another species with similar aspect is Centruroidesornatus Pocock, 1902; however, a preliminary molecular analysis of the mitochondrial gene mRNA 16S showed genetic divergence (measured as p-distance) near to 10% between these species, and lower differences between the new species with respect to C.infamatus (4.63%) and C.ruana (5.07%). The molecular evidence together with the morphological characters (integrative taxonomy) are sufficient for recognizing the Coalcomán population as a separate and valid species.

A revision of the dark-bellied, stream-dwelling snakes of the genus Hebius (Reptilia: Squamata: Natricidae) with the description of a new species from China, Vietnam and Thailand

Zootaxa ◽

10.11646/zootaxa.4911.1.1 ◽

2021 ◽

Vol 4911 (1) ◽

pp. 1-61

Author(s):

PATRICK DAVID ◽

GERNOT VOGEL ◽

TRUONG QUANG NGUYEN ◽

NIKOLAI L. ORLOV ◽

OLIVIER S. G. PAUWELS ◽

...

Keyword(s):

New Species ◽

Southern China ◽

Species Status ◽

Distribution Maps ◽

Northern Vietnam ◽

Full Species ◽

North Eastern ◽

Species of the genus Hebius Thompson, 1913 with 17 or 19 dorsal scale rows at midbody and an overall dark venter are reviewed, including the two species previously known as Parahelicops annamensis Bourret, 1934 and Pararhabdophis chapaensis Bourret, 1934. Specimens with 17 scale rows are morphologically similar to Hebius venningi (Wall, 1910), which is here redefined based on external morphological characters such as scalation, and dorsal and ventral patterns. Consequently, Natrix nigriventer Wall, 1925 is resurrected from its synonymy with Hebius venningi, whereas Natrix taronensis Smith, 1940, previously considered a subspecies of H. venningi or a full species by some authors but without justification, is here confirmed to full species status. Another group of species, mostly similar in coloration and pattern to the H. venningi group but with 19 dorsal scale rows, includes H. modestus (Günther, 1875), H. deschauenseei (Taylor, 1934) and a new species which is described herein based on specimens from northern Vietnam, southern China and north-eastern Thailand due to distinct morphological differences. We also provide updated taxonomic accounts for the species of this group, including an identification key and distribution maps.

Effectiveness of DNA barcoding markers in the description of a new and unusual calyptrate species of Myrcianthes (Myrtaceae)

Phytotaxa ◽

10.11646/phytotaxa.284.3.5 ◽

2016 ◽

Vol 284 (3) ◽

pp. 203

Author(s):

CARLOS PARRA-O. ◽

A. F. BOHÓRQUEZ-OSORIO

Keyword(s):

New Species ◽

Dna Barcoding ◽

Unusual Combination ◽

A new species, Myrcianthes roncesvallensis is described and illustrated from Andean forests of Tolima, Colombia. Due to the unusual combination of morphological characters in this new species never been found previously in a Colombian Myrtaceae, such as having a closed calyx, dichasial inflorescence and an eugenioid embryo, three DNA barcoding markers (rbcL, matK and ITS) were used to confirm the genus in which this species should be described. Taxonomic affinities of the new species within the genus are discussed.

Disentangling a cryptic species complex and defining new species within the Eumerus minotaurus group (Diptera: Syrphidae), based on integrative taxonomy and Aegean palaeogeography

Contributions to Zoology ◽

10.1163/18759866-08704001 ◽

2018 ◽

Vol 87 (4) ◽

pp. 197-225 ◽

Cited By ~ 8

Author(s):

Antonia Chroni ◽

Ana Grković ◽

Jelena Ačanski ◽

Ante Vujić ◽

Snežana Radenković ◽

...

Keyword(s):

New Species ◽

Cryptic Species ◽

Integrative Taxonomy ◽

Divergence Times ◽

Geometric Morphometric ◽

Biogeographic Patterns ◽

Phylogeographic Analysis ◽

Cryptic Species Complex ◽

Taxonomic Approach ◽

This study provides an overview of the Eumerus minotaurus taxon group, diagnosing a new species, E. anatolicus Grković, Vujić and Radenković sp. n. (Muğla, Turkey), and unraveling three cryptic species within E. minotaurus: E. karyates Chroni, Grković and Vujić sp. n. (Peloponnese, Greece), E. minotaurus Claussen and Lucas, 1988 (Crete and Karpathos, Greece) and E. phaeacus Chroni, Grković and Vujić sp. n. (Corfu and Mt Olympus, Greece; Mt Rumija, Montenegro). We applied an integrative taxonomic approach based on molecular, morphological and wing geometric morphometric data to corroborate and delimit cryptic species within the complex. In addition, we discuss the latent biogeographic patterns and speciation processes leading to configuration of the E. minotaurus group based on palaeogeographic evolution of the Aegean. Mitochondrial phylogeographic analysis suggested that speciation within the E. minotaurus group is attributable to formation of the mid-Aegean Trench and Messinian Salinity Crisis, and was integrated at the Pleistocene. We show that more accurate estimates of divergence times may be based on geological events rather than the standard arthropod mtDNA substitution rate.

Alyssum rossetii (Brassicaceae), a new species from the Aosta Valley in Italy based on morphological and genome size data

Phytotaxa ◽

10.11646/phytotaxa.360.3.7 ◽

2018 ◽

Vol 360 (3) ◽

pp. 269 ◽

Cited By ~ 4

Author(s):

STANISLAV ŠPANIEL ◽

KLAUS KAPLAN ◽

MAURIZIO BOVIO ◽

LENKA MÁRTONFIOVÁ ◽

VERONIKA CETLOVÁ

Keyword(s):

New Species ◽

Genome Size ◽

Iucn Red List ◽

Full Description ◽

New Taxon ◽

Morphological Comparison ◽

Iucn Red List Categories ◽

Alyssum rossetii, a stenoendemic madwort discovered on a mountain slope in the Valpelline valley (northern part of the Aosta Valley in the Pennine Alps), is described here as a new species. It is a perennial that is morphologically close to the Alyssum montanum-A. repens complex, with several hitherto neglected peculiar morphological characters. Here we present a full description of the new taxon, including information on its chromosome number, genome size and ecology, as well as a detailed morphological comparison of the new species with four related taxa occuring in the broader region, namely A. flexicaule, A. montanum, A. orophilum and A. rhodanense. Besides morphological differences, the new species also conspicuously differs from the other taxa in relative monoploid genome size. According to the IUCN Red List categories and criteria, it should be classified as Endangered.