Immunohistochemical Comparison of Gastrointestinal Stromal Tumor and Solitary Fibrous Tumor

2002 ◽  
Vol 126 (10) ◽  
pp. 1189-1192 ◽  
Author(s):  
Vinod B. Shidham ◽  
Mamatha Chivukula ◽  
Dilip Gupta ◽  
R. Nagarjun Rao ◽  
Richard Komorowski
Keyword(s):  
Smooth Muscle ◽  
Gastrointestinal Stromal Tumors ◽  
Smooth Muscle Actin ◽  
S100 Protein ◽  
Muscle Actin ◽  
Santa Cruz ◽  
Stromal Tumors ◽  
Solitary Fibrous Tumors ◽  
Positive Immunoreactivity ◽  
Fibrous Tumor

Abstract Context.—The differential diagnosis of gastrointestinal stromal tumors (GIST) and solitary fibrous tumors (SFT) may be a diagnostic challenging because of overlapping clinicopathologic features. Many studies have shown consistent immunoreactivity for CD117 (c-Kit) in GIST. However, only a few studies have evaluated CD117 expression in SFT, and these studies have used an antibody from Santa Cruz Biotechnology. In non-GIST lesions, reactivity with this antibody has been shown to differ from that with a CD117 antibody from Dako Corporation. The immunoreactivity of SFT with the Dako CD117 antibody has not been reported. Conversely, CD99 is a marker for SFT, and its expression in GIST has not been evaluated. Objective.—To study the immunohistochemical profiles of GIST and SFT to evaluate their diagnostic overlap. Design.—We studied the immunoreactivity of 27 unequivocal GIST and 19 unequivocal extra-abdominal SFT for CD117, CD34, CD99, α-smooth muscle actin, vimentin, CD31, S100 protein, and muscle-specific actin. All antibodies, including CD117, were from Dako Corporation. Results.—We found positive immunoreactivity for CD117 in 100% of GIST and none of SFT; for CD34 in 89% of GIST, and 100% of SFT; for CD99 in 89% of GIST and 100% of SFT; for α-smooth muscle actin in 48% of GIST and 31% of SFT; for vimentin in 89% of GIST and 90% of SFT; and for muscle-specific actin in 22% of GIST and none of SFT. None of the GIST or SFT showed immunoreactivity for CD31 and S100 protein. Conclusions.—The major difference between GIST and SFT was strong CD117 immunoexpression in all GIST and an absence of this expression in all SFT. With the exception of muscle-specific actin, the prevalence of immunoreactivity for the markers studied did not differ substantially between these 2 tumors. We conclude that GIST and SFT show distinctly divergent immunoprofiles with respect to CD117 and muscle-specific actin.

Gastrointestinal Stromal Tumors and Leiomyomas in the Dog: A Histopathologic, Immunohistochemical, and Molecular Genetic Study of 50 Cases

2003 ◽  
Vol 40 (1) ◽  
pp. 42-54 ◽  
Author(s):  
D. Frost ◽  
J. Lasota ◽  
M. Miettinen
Keyword(s):  
Smooth Muscle ◽  
Gastrointestinal Stromal Tumors ◽  
Smooth Muscle Actin ◽  
Abdominal Cavity ◽  
Clinical Signs ◽  
Muscle Actin ◽  
Molecular Genetic Study ◽  
Stromal Tumors ◽  
Pathologic Features ◽  
S 100

Fifty canine gastrointestinal (GI) mesenchymal tumors were examined to determine the occurrence of leiomyomas (LM) and GI stromal tumors and to compare their clinicopathologic features. Twenty-one tumors (42%) were histologically reclassified as gastrointestinal stromal tumors (GISTs) and 29 tumors (58%) as LMs on the basis of their histologic similarity with homologous human tumors. The GISTs occurred equally in males and females, with a mean age of 11 years (range 5–14 years). Five GISTs (24%) were associated with clinical signs and six (29%) had metastasis in liver or abdominal cavity. The GISTs occurred in large intestine (10, 48%), small bowel (six, 29%), stomach (four, 19%), and mesentery of small intestine (one, 5%). Histologically, they were highly cellular spindle, or less commonly epithelioid tumors with mitotic rates ranging from 0 to 19 per 10 HPF. Eleven tumors (52%) were positive for CD117 (KIT); seven (33%) were positive for smooth muscle actin but none for desmin and S-100 protein. Sequences of KIT exon 11, often mutated in human GISTs, were evaluated from four GISTs. Deletion of Try556-Lys557 coexisting with duplication of Gln555 in one case of GIST and T to C transition resulting in substitution of Pro for Leu575 in another were identified. The LMs occurred predominantly in males (82%) with a mean age of 11 years (range 8–17 years). Nine tumors (31%) had associated clinical signs. They occurred in the stomach (22, 76%), esophagus (four, 14%), and intestines (three, 10%); all were paucicellular, had no mitoses, and were composed of mature smooth muscle cells. Twenty-eight (97%) were positive for smooth muscle actin and 18(62%) for desmin but none for CD117 and S-100. Both GISTs and true LMs occur in the GI tract of dogs. Both tumors have distinctive pathologic features.

Immunohistochemical Staining Characteristics of Canine Gastrointestinal Stromal Tumors

1997 ◽  
Vol 34 (4) ◽  
pp. 303-311 ◽  
Author(s):  
R. G. LaRock ◽  
P. E. Ginn
Keyword(s):  
Smooth Muscle ◽  
Small Intestine ◽  
Immunohistochemical Staining ◽  
Gastrointestinal Stromal Tumors ◽  
Smooth Muscle Actin ◽  
Muscle Actin ◽  
Stromal Tumors ◽  
S 100 ◽  
Microscopic Features ◽  
Microscopic Diagnosis

Sections from 35 formalin-fixed, paraffin-embedded, canine gastrointestinal stromal tumors consisting of 14 leiomyomas (five stomach, three small intestine, two colon, four rectum), 18 leiomyosarcomas (one stomach, five small intestine, nine cecum, three rectum), two undifferentiated sarcomas (two stomach), and one neurofibrosarcoma (small intestine) were examined for the expression of vimentin, S-100 protein, α-smooth muscle actin, and desmin via immunoperoxidase methodology using an avidin-biotin complex technique. The leiomyomas were 4/14 (29%) vimentin-positive, 3/14 (21%) S-100 protein-positive, 10/14 (71%) α-smooth muscle actin-positive and 13/14 (93%) desmin-positive. Leiomyosarcomas were 18/18 (100%) vimentin-positive, 11/18 (61%) S-100 protein-positive, 9/18 (50%) α-smooth muscle actin-positive, and 15/18 (83%) desmin-positive. The undifferentiated sarcomas were 2/2 (100%) vimentin-positive, 2/2 (100%) S-100 protein-positive, 1/2 (50%) α-smooth muscle actin-positive, and 0/2 (0%) desmin-positive. The neurofibrosarcoma was vimentin and S-100 protein-positive and α-smooth muscle actin- and desmin-negative. Thirty-one of thirty-five (89%) of all neoplasms demonstrated reactivity for either desmin and/or α-smooth muscle actin. S-100 protein reactivity occurred in 17/35 (49%) of all specimens. Lack of desmin and α-smooth muscle actin reactivity occurred in 4/35 (11%) of all specimens, all of which were vimentin-positive. The immunohistochemical results indicate that the majority of canine gastrointestinal stromal tumors (GIST) with light microscopic features of smooth muscle cells have immunohistochemical staining patterns supporting smooth muscle differentiation. Vimentin reactivity correlated with a light microscopic diagnosis of malignancy. The lack of smooth muscle cell markers in some tumors and the high percentage of cases positive for S-100 protein may suggest a more complex histogenesis or differentiation for subgroups of these tumors.

scholarly journals Leiomyosarcoma of the colon

2015 ◽  
Vol 68 (11-12) ◽  
pp. 413-417 ◽  
Author(s):  
Vlado Janevski ◽  
Redzep Selmani ◽  
Vesna Janevska ◽  
Liljana Spasevska ◽  
Julija Zhivadinovik
Keyword(s):  
Smooth Muscle ◽  
Gastrointestinal Stromal Tumors ◽  
Histopathological Examination ◽  
Smooth Muscle Actin ◽  
Abdominal Mass ◽  
Neuron Specific Enolase ◽  
Muscle Actin ◽  
Ascending Colon ◽  
Mesenchymal Tumors ◽  
Stromal Tumors

Introduction. Gastrointestinal stromal tumors are the most common mesenchymal tumors of the digestive tract. Leiomyosarcomas of the gastrointestinal tract are rare mesenchymal neoplasms which grossly and histologically resemble gastrointestinal stromal tumors. They may be differentiated from gastrointestinal stromal tumors by using immunohistochemistry and they are typically positive for ? smooth muscle actin and desmin and negative for c-kit, CD34 and DOG1.1. They often express calponin and h-caldesmon. Case Report. We present a case of a 59-year-old male with anemia, weight loss, intermittent abdominal pain and right abdominal mass. Colonoscopy revealed an exophytic ulcerated neoplastic mass in the ascending colon and abdominal computed tomography scan showed an ill-defined heterogeneous tumor mass which surrounded almost the whole ascending colon. The patient underwent right hemicolectomy and partial resection of ileum. Histopathological examination revealed a leiomyosarcoma composed of atypical spindle cells positive for ? smooth muscle actin, desmin and vimentin, and negative for c-kit, CD34, S100 and neuron specific enolase. The patient is alive 8 months after the operation, undergoing chemotherapy. Conclusion. We have concluded that the multimodal approach comprising chemotherapy and complete surgical resection controls the leiomyosarcomas.

Malignant Solitary Fibrous Tumor of the Pleura With Liposarcomatous Differentiation

2001 ◽  
Vol 125 (3) ◽  
pp. 406-409
Author(s):  
Hongwei Bai ◽  
Bassam I. Aswad ◽  
Henning Gaissert ◽  
Douglas R. Gnepp
Keyword(s):  
Smooth Muscle ◽  
Tumor Cells ◽  
Solitary Fibrous Tumor ◽  
Smooth Muscle Actin ◽  
Mitotic Rate ◽  
Muscle Actin ◽  
Solitary Fibrous Tumors ◽  
Collagenous Stroma ◽  
Well Differentiated ◽  
Fibrous Tumor

Abstract Pleural solitary fibrous tumors (SFTs) are uncommon tumors. Although these tumors have been well characterized, malignant pleural SFTs with liposarcomatous differentiation have not been reported. We report an unusual malignant pleural SFT intermixed with foci of well-differentiated liposarcoma. The patient was a 66-year-old, white man with a large, solid right pleural mass that measured 13.5 × 10.3 × 8.5 cm. The tumor was composed of spindle-shaped and plump cells embedded in dense collagenous stroma. The tumor cells were arranged in interlacing fascicles or in a patternless pattern. Marked nuclear atypia, a high mitotic rate (21 mitoses per 10 high-power fields), and areas of prominent necrosis were evident. In addition, numerous adipocytes mixed with typical lipoblasts were seen scattered throughout portions of the tumor. Immunohistochemistry revealed the tumor cells were strongly positive for CD34 and vimentin and negative for cytokeratin, desmin, smooth muscle actin (IA4), and S100. To the best of our knowledge, this case represents the first example of a malignant SFT with liposarcomatous differentiation.

Solitary fibrous tumor of the scalp in a child

2013 ◽  
Vol 11 (1) ◽  
pp. 79-81 ◽  
Author(s):  
Tony Rizk ◽  
Adnan Awada ◽  
Amer Sebaaly ◽  
Roula Hourani
Keyword(s):  
Immunohistochemical Staining ◽  
Smooth Muscle Actin ◽  
S100 Protein ◽  
Muscle Actin ◽  
Solitary Fibrous Tumors ◽  
Mesenchymal Neoplasms ◽  
Wide Range ◽  
Fibrous Tumor ◽  
Scalp Mass

Solitary fibrous tumors (SFTs) are rare mesenchymal neoplasms that arise most commonly in the pleura but have been increasingly reported in extrapleural sites. The authors report the case of an SFT of the scalp that manifested as an enlarging scalp mass in a 2-year-old boy. The mass was surgically excised. Histological examination showed it to be composed of fusiform cells of variable cellularity with a central hyalinization zone. Immunohistochemical staining was positive for CD34 and negative for smooth muscle actin, S100 protein, desmin, and h-caldesmon. On 1-year follow-up, there was no recurrence of the mass. This case presented some diagnostic difficulty because of the wide range of possible diagnoses for a pediatric scalp mass; however, the distinct immunohistochemical profile helped to eliminate other more frequent fibrous tumors of the scalp. The behavior of scalp SFTs is usually benign, but their course can be unpredictable, and recognition of these lesions is essential.

scholarly journals Colitis and “diaphragm disease” of the colon in hemodialysis patient due to prolonged use of non-steroidal anti-inflammatory drug

2015 ◽  
Vol 68 (11-12) ◽  
pp. 418-420 ◽  
Author(s):  
Nada Dimkovic ◽  
Danijela Bojic ◽  
Petar Svorcan ◽  
Aleksandar Jankovic ◽  
Petar Djuric ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Gastrointestinal Stromal Tumors ◽  
Histopathological Examination ◽  
Smooth Muscle Actin ◽  
Abdominal Mass ◽  
Neuron Specific Enolase ◽  
Muscle Actin ◽  
Ascending Colon ◽  
Mesenchymal Tumors ◽  
Stromal Tumors

Introduction. Gastrointestinal stromal tumors are the most common mesenchymal tumors of the digestive tract. Leiomyosarcomas of the gastrointestinal tract are rare mesenchymal neoplasms which grossly and histologically resemble gastrointestinal stromal tumors. They may be differentiated from gastrointestinal stromal tumors by using immunohistochemistry and they are typically positive for ? smooth muscle actin and desmin and negative for c-kit, CD34 and DOG1.1. They often express calponin and h-caldesmon. Case Report. We present a case of a 59-year-old male with anemia, weight loss, intermittent abdominal pain and right abdominal mass. Colonoscopy revealed an exophytic ulcerated neoplastic mass in the ascending colon and abdominal computed tomography scan showed an ill-defined heterogeneous tumor mass which surrounded almost the whole ascending colon. The patient underwent right hemicolectomy and partial resection of ileum. Histopathological examination revealed a leiomyosarcoma composed of atypical spindle cells positive for ? smooth muscle actin, desmin and vimentin, and negative for c-kit, CD34, S100 and neuron specific enolase. The patient is alive 8 months after the operation, undergoing chemotherapy. Conclusion. We have concluded that the multimodal approach comprising chemotherapy and complete surgical resection controls the leiomyosarcomas.

Gastrointestinal Stromal Tumors of the Equine Cecum

2001 ◽  
Vol 38 (2) ◽  
pp. 242-246 ◽  
Author(s):  
S. Hafner ◽  
B. G. Harmon ◽  
T. King
Keyword(s):  
Gastrointestinal Stromal Tumors ◽  
Smooth Muscle Actin ◽  
S100 Protein ◽  
Neuron Specific Enolase ◽  
Postmortem Examination ◽  
Muscle Actin ◽  
Neurosecretory Granules ◽  
Stromal Tumors ◽  
Spindle Cells ◽  
Cell Processes

Ten cecal tumors were identified during the postmortem examination of seven horse carcasses at slaughter (one horse had three tumors). The multinodular and hemorrhagic tumors ranged from 1 to 10 cm in diameter and consisted of spindle cells arranged in thin, interconnected trabeculae that were often separated by sinuses filled with mucinous fluid, erythrocytes, and siderophages. Spindle cells of all tumors were immuno-positive for vimentin, neuron-specific enolase, and c-kit protein but lacked reactivity with antibodies to glial fibrillary acidic protein, S100 protein, and desmin. In one tumor, spindle cells diffusely bound antibodies to synaptophysin. Most tumors contained focal reactivity to smooth muscle actin antibodies; one tumor reacted diffusely. Ultrastructurally, tumor cells were connected by desmosome-like structures and exhibited extended cell processes; some contained dense core neurosecretory granules. These equine stromal tumors appeared to share some characteristics with human gastrointestinal stromal tumors.

Immunohistochemical diagnostic of hibernoma in dog

2015 ◽  
Vol 18 (1) ◽  
pp. 233-236 ◽  
Author(s):  
S. Dzimira ◽  
V. Kapuśniak ◽  
J.A. Madej
Keyword(s):  
Veterinary Medicine ◽  
Smooth Muscle ◽  
Smooth Muscle Actin ◽  
S100 Protein ◽  
Muscle Actin ◽  
Human Pathology

Abstract The diagnosis of hibernoma is uncommon in veterinary medicine. In this report, we present an attempt to confirm hibernoma diagnosed in dogs by applying immunohistochemical tests routinely used in human pathology i.e. antibodies specific to protein S100, protein CD31, or smooth muscle actin (SMA).

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