In vitro plant regeneration in rough lemon (Citrus jambhiri Lush) by direct organogenesis

2011 ◽  
Vol 10 (63) ◽  
pp. 13724-13728 ◽  
Author(s):  
S Rattanpal H ◽  
Gag ◽  
Kaur eep ◽  
Gupta Monika
Author(s):  
Ashrafi Papry ◽  
Sayeda Sultana ◽  
Goutam Deb ◽  
Mohammed Bhuiyan

2010 ◽  
Vol 19 (2) ◽  
pp. 199-206
Author(s):  
Rakha Hari Sarker ◽  
Bivas Kumar Sarkar ◽  
Bivas Kumar Sarkar ◽  
M. I. Hoque ◽  
M. I. Hoque

An in vitro regeneration system for two varieties of white jute (Corchorus capsularis L.) namely, BJC-7370 and BJC-83 was developed.  The regeneration protocol was based upon direct organogenesis from seedling-derived explants such as cotyledon with petioles, cotyledonary node. Shoot regeneration was achieved from cotyledon with petiole and cotyledonary node through use of MS supplemented with 0.5 mg/l BAP and 1.0 mg/l IAA for BJC-7370 while for BJC-83 shoot initiation was obtained on 1.25 mg/l BAP and 0.25 mg/l NAA using the same explants. Elongation of shoots was achieved on MS containing 0.2 mg/l BAP for the two said jute varieties. Regenerated excised shoots developed effective in vitro root system on half strength of MS supplemented with 0.3 mg/l IBA for both the varieties. The in vitro grown plantlets were transferred to soil for acclimation. These plants grew up to maturity, flowered and produced seeds identical to the control plants.  Key words:  Corchorus capsularies, In vitro plant regeneration, seedling explants D.O.I. 10.3329/ptcb.v19i2.5437 Plant Tissue Cult. & Biotech. 19(2): 199-206, 2009 (December)


2012 ◽  
Vol 47 (2) ◽  
pp. 187-190
Author(s):  
Chapol Kumar Roy ◽  
John Liton Munshia ◽  
Nadira Begum ◽  
Rahima Khatun ◽  
AKM Sayeed Hassanb

An efficient protocol was developed for in vitro plant regeneration of a popularly used anti-diabetic medicinal plant, Coccinea cordifolia (Linn.) Cogn.(Cucurbitaceae) through direct organogenesis using shoot tip and nodal explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP, in which 88.2% of nodal explants responded to produce maximum number (6.2 ± 0.58) of shoots per culture. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IBA. The survival rate of regenerated plantlets was 85%.   DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11450   Bangladesh J. Sci. Ind. Res. 47(2), 187-190, 2012  


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