scholarly journals In vitro Plant Regeneration from Seedling-derived Explants of two Cultivars of White Jute (Corchorus capsularies L.)

2010 ◽  
Vol 19 (2) ◽  
pp. 199-206
Author(s):  
Rakha Hari Sarker ◽  
Bivas Kumar Sarkar ◽  
Bivas Kumar Sarkar ◽  
M. I. Hoque ◽  
M. I. Hoque
Keyword(s):  
Plant Regeneration ◽  
In Vitro Regeneration ◽  
Cotyledonary Node ◽  
Direct Organogenesis ◽  
In Vitro Plant Regeneration ◽  
Regeneration System ◽  
Shoot Initiation ◽  
Corchorus Capsularis ◽  
In Vitro Plant

An in vitro regeneration system for two varieties of white jute (Corchorus capsularis L.) namely, BJC-7370 and BJC-83 was developed.  The regeneration protocol was based upon direct organogenesis from seedling-derived explants such as cotyledon with petioles, cotyledonary node. Shoot regeneration was achieved from cotyledon with petiole and cotyledonary node through use of MS supplemented with 0.5 mg/l BAP and 1.0 mg/l IAA for BJC-7370 while for BJC-83 shoot initiation was obtained on 1.25 mg/l BAP and 0.25 mg/l NAA using the same explants. Elongation of shoots was achieved on MS containing 0.2 mg/l BAP for the two said jute varieties. Regenerated excised shoots developed effective in vitro root system on half strength of MS supplemented with 0.3 mg/l IBA for both the varieties. The in vitro grown plantlets were transferred to soil for acclimation. These plants grew up to maturity, flowered and produced seeds identical to the control plants.  Key words:  Corchorus capsularies, In vitro plant regeneration, seedling explants D.O.I. 10.3329/ptcb.v19i2.5437 Plant Tissue Cult. & Biotech. 19(2): 199-206, 2009 (December)

scholarly journals Development-Related miRNA Expression and Target Regulation during Staggered In Vitro Plant Regeneration of Tuxpeño VS-535 Maize Cultivar

2019 ◽  
Vol 20 (9) ◽  
pp. 2079 ◽  
Author(s):  
Brenda A. López-Ruiz ◽  
Vasti T. Juárez-González ◽  
Estela Sandoval-Zapotitla ◽  
Tzvetanka D. Dinkova
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
In Vitro Regeneration ◽  
In Vitro Plant Regeneration ◽  
Embryogenic Calli ◽  
Target Regulation ◽  
In Vitro Plant ◽  
The Impact ◽  
Embryogenic Tissues

In vitro plant regeneration addresses basic questions of molecular reprogramming in the absence of embryonic positional cues. The process is highly dependent on the genotype and explant characteristics. However, the regulatory mechanisms operating during organ differentiation from in vitro cultures remain largely unknown. Recently, miRNAs have emerged as key regulators during embryogenic callus induction, plant differentiation, auxin responses and totipotency. Here, we explored how development-related miRNA switches the impact on their target regulation depending on physiological and molecular events taking place during maize Tuxpeño VS-535 in vitro plant regeneration. Three callus types with distinctive regeneration potential were characterized by microscopy and histological preparations. The embryogenic calli (EC) showed higher miRNA levels than non-embryogenic tissues (NEC). An inverse correlation for miR160 and miR166 targets was found during EC callus induction, whereas miR156, miR164 and miR394 displayed similar to their targets RNA accumulation levels. Most miRNA accumulation switches took place early at regenerative spots coincident with shoot apical meristem (SAM) establishment, whereas miR156, miR160 and miR166 increased at further differentiation stages. Our data uncover particular miRNA-mediated regulation operating for maize embryogenic tissues, supporting their regulatory role in early SAM establishment and basipetala growth during the in vitro regeneration process.

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