scholarly journals A PCR ITS-RFLP method for identifying fungal contamination of date palm (Phoenix dactylifera L.) tissue cultures

2013 ◽  
Vol 12 (32) ◽  
pp. 5054-5059 ◽  
Author(s):  
H Abass Mohammed
Author(s):  
Ahmed Madi Waheed Al-Mayahi

Abstract Background Iron chelate sources and their concentrations are important factors in in vitro propagation of date palm. This study’s objective was to investigate the effect of the iron chelated form on the growth and development of tissue cultures of Barhee cultivar. Results The addition of FeEDDHA to the culture medium was more effective than FeEDTA on callus growth, shoot regeneration, and the number of shoots per jar, where the best result (220.8mg callus, 86.67% and 17.2 shoots per jar, respectively) was obtained by using 93.5 mg L−1 FeEDDHA (5.6 mg L−1 Fe), compared with other treatments. The results also indicate that using 93.5 mg L−1 FeEDDHA (5.6 mg L−1 Fe) as a supplement can decrease antioxidant enzymes CAT and POD activity compared to the rest of the treatments. Medium equipped with 187.0 mg L−1 FeEDDHA (11.2 mg L−1Fe) had the highest rooting percentage and number of roots per shoot than other treatments. The biochemical analysis results showed that treatments with FeEDDHA of 280.5 mg L−1 (16.8 mg L−1 Fe) and 187.0 mg L−1 (11.2 mg L−1Fe) significantly increased the iron content. The results showed that shoot maximum chlorophyll and endogenous IAA level content were recorded in a medium supplemented with 187.0 mg L−1 FeEDDHA (11.2 mg L−1Fe) as Fe source. Conclusion FeEDDHA used in the present study was proven to be a promising iron chelate source in comparison with the FeEDTA sources.


LWT ◽  
2021 ◽  
pp. 111762
Author(s):  
Ibrahim A. Almusallam ◽  
Isam A. Mohamed Ahmed ◽  
Elfadil E. Babiker ◽  
Fahad Y. Al-Juhaimi ◽  
Ali Saleh ◽  
...  

2017 ◽  
Vol 22 (9) ◽  
pp. 1444 ◽  
Author(s):  
Fahad Jaber Alatawi ◽  
Muhammad Kamran

A comprehensive survey was conducted at different provinces of Saudi Arabia (SA) to investigate predacious prostigmatid mites (Acari: Trombidiformes: Prostigmata) mainly collected from the date palm, Phoenix dactylifera L. (Arecaceae) agro-ecosystem. A total of 41 species belonging to 28 genera in 14 families are recorded in the present study. Out of these, three families, 18 genera and 36 species are new to the mite fauna of SA. The new species, Neobonzia tabukensis sp.nov. (Cunaxidae), Cheletomimus (Philippicheyla) taifensis sp.nov. (Cheyletidae) are described and illustrated. A checklist of predatory prostigmatid mites from SA is given. A key to all terrestrial predatory prostigmatid mites, excluding Parasitegona and Heterostigmatina, reported from SA is provided. Also, a key to world species of the subgenus Philippicheyla Corpuz-Raros of the genus Cheletomimus Oudemans is given.


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