Detection and typing of human papillomavirus (HPV) in condyloma acuminatum and bowenoid papulosis HybriBio HPV GenoArray test kit, real-time polymerase chain reaction (PCR) and sequencing

IntroductionThe human papillomavirus (HPV) family is characterized by minimal genotypic differences corresponding to different virus types. The aim of this study was to detect the HPV coinfections and the inner genotype in a series of 336 cervical-vaginal samples.MethodsA total of 336 cervical-vaginal samples were taken from 2007 to 2009 using specific molecular techniques such as molecular sequencing and hybridizations. The genome amplification of the L1 open reading frame was analyzed by real-time polymerase chain reaction; direct sequencing was performed by SYBR green fluorescent molecule and degenerate primers MY09 and MY11. The HPV genotyping was accomplished via oligonucleotide probe hybridization. The phylogenetic correlations in coinfections were analyzed by sequence homology of the L1 genomic region. Identified genotypes were then compared.ResultsHuman papillomavirus positivity was observed in 125 cases (37.2%), with 21 cases (16.8%) of HPV presence in coinfections. Coinfections involved HPV 16 genotype (8 cases) and HPV 18 (5 cases). The HPV 16 infection was mainly associated with genotypes with a lower-than-broad sequence homology, so the HPV 18 was linked to genotypes represented in the opposite phylogenetic tree.ConclusionsThe combined and steady use of diagnostic procedures, such as real-time polymerase chain reaction, molecular hybridization, direct sequencing, and HPV genotyping test, allow accurate diagnosis of monoinfections and coinfections. This may faciliate the development of specific viral tests and prophylactic anti-HPV vaccines.

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cobas®4800 HPV Test, a real-time polymerase chain reaction assay for the detection of human papillomavirus in cervical specimens

Expert Review of Molecular Diagnostics ◽

10.1586/14737159.2014.865521 ◽

2013 ◽

Vol 14 (1) ◽

pp. 5-16 ◽

Cited By ~ 16

Author(s):

Sandra D Isidean ◽

François Coutlée ◽

Eduardo L Franco

Keyword(s):

Polymerase Chain Reaction ◽

Human Papillomavirus ◽

Real Time ◽

Polymerase Chain Reaction Assay ◽

Chain Reaction ◽

Hpv Test ◽

Polymerase Chain

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Absolute quantitative real-time polymerase chain reaction for the measurement of human papillomavirus E7 mRNA in cervical cytobrush specimens

Infectious Agents and Cancer ◽

10.1186/1750-9378-2-8 ◽

2007 ◽

Vol 2 (1) ◽

pp. 8 ◽

Cited By ~ 15

Author(s):

Michael E Scheurer ◽

Laura M Dillon ◽

Zhuo Chen ◽

Michele Follen ◽

Karen Adler-Storthz

Keyword(s):

Polymerase Chain Reaction ◽

Human Papillomavirus ◽

Real Time ◽

Chain Reaction ◽

Polymerase Chain

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Evaluation of SARS-CoV2 antibody Rapid Diagnostic Test kits (RDTs) and Real Time-Polymerase Chain Reaction (Rt-PCR) for COVID-19 Diagnosis in Kaduna, Nigeria

10.1101/2020.11.24.20231324 ◽

2020 ◽

Author(s):

Oluwafemi Ige ◽

Ayuba Sunday Buru ◽

Tanko Zainab Lamido ◽

Tahir Mohammed ◽

Livingstone Dogara ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Real Time ◽

Diagnostic Test ◽

Venous Blood ◽

World Health ◽

Rt Pcr ◽

Chain Reaction ◽

The Real ◽

Test Kit ◽

Polymerase Chain

AbstractThe emergence of the RNA virus SARS-CoV2, the causative agent of COVID-19 and its declaration by the World Health Organization (WHO) as a pandemic has disrupted the delicate balance in health indices globally. Its attendant immune dysregulation and pathobiology is still evolving. Currently, real time PCR is the gold standard diagnostic test, however there are several invalidated antibody-based tests available for possible community screening. With ongoing community transmission in Nigeria, neither the true burden of COVID-19 nor the performance of these kits is presently known. This study therefore, compared the performance of the SARS CoV2 antibody test and the real time Polymerase Chain Reaction (Rt-PCR) in the diagnosis of COVID-19. For the purpose of this evaluation, we used the diagnostic test kit by Innovita® Biological Technology CO., LTD China, a total of 521 venous blood samples were collected from consenting patients for the SARS COVID-19 rapid diagnostic kit and Oral and Nasopharyngeal swabs were collected and analyzed using the real time Polymerase chain reaction technique for nucleic acid detection and quantification.

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