scholarly journals Production of Cellulolytic Enzymes by Aspergillus flavus Using Solid State Fermentation Based on Sugarcane Bagasse

2013 ◽  
Vol 3 (2) ◽  
pp. 25-28 ◽  
Author(s):  
Onofre Sideney Becker
2015 ◽  
Vol 18 (1) ◽  
pp. 5-9 ◽  
Author(s):  
Hoda Hanem Mohamed Fadel ◽  
Manal Gomaa Mahmoud ◽  
Mohsen Mohamed Selim Asker ◽  
Shereen Nazeh Lotfy

2016 ◽  
Vol 29 (1) ◽  
pp. 222-233 ◽  
Author(s):  
TAMIRES CARVALHO DOS SANTOS ◽  
GEORGE ABREU FILHO ◽  
AILA RIANY DE BRITO ◽  
AURELIANO JOSÉ VIEIRA PIRES ◽  
RENATA CRISTINA FERREIRA BONOMO ◽  
...  

ABSTRACT: Prickly palm cactus husk was used as a solid-state fermentation support substrate for the production of cellulolytic enzymes using Aspergillus niger and Rhizopus sp. A Box-Behnken design was used to evaluate the effects of water activity, fermentation time and temperature on endoglucanase and total cellulase production. Response Surface Methodology showed that optimum conditions for endoglucanase production were achieved at after 70.35 h of fermentation at 29.56°C and a water activity of 0.875 for Aspergillus niger and after 68.12 h at 30.41°C for Rhizopus sp. Optimum conditions for total cellulase production were achieved after 74.27 h of fermentation at 31.22°C for Aspergillus niger and after 72.48 h and 27.86°C for Rhizopus sp. Water activity had a significant effect on Aspergillus niger endoglucanase production only. In industrial applications, enzymatic characterization is important for optimizing variables such as temperature and pH. In this study we showed that endoglucanase and total cellulase had a high level of thermostability and pH stability in all the enzymatic extracts. Enzymatic deactivation kinetic experiments indicated that the enzymes remained active after the freezing of the crude extract. Based on the results, bioconversion of cactus is an excellent alternative for the production of thermostable enzymes.


2019 ◽  
Vol 17 ◽  
pp. 1-6 ◽  
Author(s):  
Gabriella Soares Borges Salomão ◽  
Jacyele Clarindo Agnezi ◽  
Larissa Bastos Paulino ◽  
Luana Borchardt Hencker ◽  
Taísa Shimosakai de Lira ◽  
...  

2011 ◽  
Vol 41 (12) ◽  
pp. 2210-2216 ◽  
Author(s):  
Tamires Carvalho dos Santos ◽  
Ingrid Souza Cavalcanti ◽  
Renata Cristina Ferreira Bonomo ◽  
Nivio Batista Santana ◽  
Marcelo Franco

The present paper analyses the effects of water activity (0.88, 0.94 and 0.97) and of fermentation time (24, 48, 72, 96 and 120 hours) on the kinetic activity of enzymes cellulolytic, produced during the solid state fermentation of waste from the improvement of mango, with the aid of fungus species Aspergillus niger. Solid state fermentation was carried out at 35°C inside a bacteriological incubator. The statistical results indicated that the best activity for enzyme CMCase was 7.26U g-1 after 74.51 hours of fermentation, whereas for enzyme FPase was 2.55U g-1 after 98.52 hours, both presenting best results in approximately 0.928 of water activity. Pareto charts have showed that fermentation time has greater effect over the activity of enzyme CMCase, while the water activity variable has greater effect over enzyme FPase activity. During fermentation the fungus synthesized the enzymes without the need of inductors other than mango residue and water.


1993 ◽  
Vol 44 (1) ◽  
pp. 5-8 ◽  
Author(s):  
M.T. Baca ◽  
E. Esteban ◽  
G. Almendros ◽  
A.J. Sanchez-Raya

2012 ◽  
Vol 6 (1) ◽  
pp. 142-152 ◽  
Author(s):  
Ursula Fabiola Rodríguez-Zúñiga ◽  
Sonia Couri ◽  
Victor Bertucci Neto ◽  
Silvio Crestana ◽  
Cristiane Sanchez Farinas

2013 ◽  
Vol 22 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Tamires Carvalho dos Santos ◽  
George Abreu Filho ◽  
Ana Carolina Oliveira ◽  
Thiago José Onório Rocha ◽  
Fabiano de Paula Pereira Machado ◽  
...  

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