A First-Derivative Spectrophotometric Method for the Determination of Ciprofloxacin Hydrochloride in Ophthalmic Solution

Abstract A highly selective and sensitive derivative spectrophotometric method has been developed for the determination of Fe(II) and Ni(II) in different mineral vitamins. The method is based on the formation of binary complexes of Fe(II) and Ni(II) with 2,4-diamino-5-(2-hydroxy-5-nitrophenylazo) benzenesulfonic acid sodium salt (Mordant Brown 33) at pH 5.6 with Tween 20. At max 512 and 493 nm, the molar absorbtivity was 2.09 104 and 0.58 104 L/mol cm for Fe(II) and Ni(II), respectively. Fe(II) can be determined in the range 0.552.79 g/mL in the presence of 2.92 g/mL Ni, and Ni(II) can be determined in the range 1.173.5 g/mL in the presence of 2.79 g/mL Fe(II) in the presence of Tween 20 (4). The detection limits were 9.0 and 15.0 ng/mL for Fe(II) and Ni(II), respectively. The effect of foreign ions was elucidated. The RSD values were, in all instances, less than 1.3. The proposed method was successfully applied for the simultaneous determination of Fe(II) and Ni(II) in different mineral vitamins.

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Derivative spectrophotometric for simultaneous estimation of propranolol hydrochloride and hydrochlorothiazide in synthetic mixture

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i1.1922 ◽

2020 ◽

Vol 11 (1) ◽

pp. 967-973

Author(s):

Suha Sabri Al Samarrai ◽

Khalaf F. Alsamarrai ◽

Eman Thiab Alsamarrai

Keyword(s):

Spectrophotometric Method ◽

Synthetic Mixture ◽

Simultaneous Estimation ◽

Propranolol Hydrochloride ◽

Uv Spectrum ◽

First Derivative ◽

Analytical Results ◽

Good Repeatability

A simple, sensitive and economical spectrophotometric method for simultaneously estimation of PRO and HCTZ. The first derivative (D1) of the UV spectrum was used in the determination of both drugs in their synthetic mixtures. The Peak to baseline and Peak area at suitable wavelengths were used in the study. The linearity of both drugs was up to a concentration of (5-40 µg/ml). The analytical results of the estimation of PRO were, Rec% 97.179-102.424% and RSD% 0.001-4.996 %. While for estimation of HCTZ were, Rec% between 95.406-103.681% and RSD% 0.001-3.676%. The method was accurate, good repeatability and successfully applied in the estimation of both drugs in their synthetic mixtures.

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Analytical Spectrometric Study For Determining Dapagliflozin Propanediol Monohydrate Individually Or In Presence Of Metformin Hydrochloride In Tablets Formulation

JOURNAL OF ADVANCES IN CHEMISTRY ◽

10.24297/jac.v17i.8812 ◽

2020 ◽

Vol 17 ◽

pp. 80-87

Author(s):

SAAD ANTAKLI ◽

Raghad Kabbani ◽

And Rama Labban

Keyword(s):

Regression Analysis ◽

Spectrophotometric Method ◽

Limit Of Detection ◽

Correlation Coefficients ◽

Metformin Hydrochloride ◽

Spectrometric Study ◽

Limit Of Quantification ◽

First Derivative ◽

Linearity Range

First simple spectrophotometric method was developed and applied to determine Dapagliflozin Propanediol Monohydrate by Zero Spectrophotometry and First Derivative Spectrophotometric method for determining of Dapagliflozin Propanediol Monohydrate (DAPA) in the presence of Metformin Hydrochloride (MET). Zero spectrophotometric (ZS) was applied for the determination of (DAPA) at 223.5 nm. Linearity range was (2.61– 31.23) µg/mL. Regression analysis showed a good correlation coefficients R2 = 0.9989. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.569 µg/mL and 1.724 µg/mL, respectively. Derivative spectrophotometric (1DS) was applied for the determination of (DAPA) in the presence (MET). (DAPA) was determined at 233 nm (1D233). Linearity ranges were (5.21 – 41.64) µg/mL for (DAPA). Regression analysis showed a good correlation coefficients R2 = 0.9994. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.732 µg/mL and 2.218 µg/mL for (DAPA). The proposed Zero spectrophotometry method was applied to analysis individual (DAPA), and the derivative (1D233) method was applied to analysis (DAPA) individually or with (MET) combination in Syrian trademark drugs.

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Determination of Candesartan Cilexetil in Tablet Dosage Forms and Dissolution Testing Samples by First Derivative UV Spectrophotometric Method

Analytical Letters ◽

10.1080/00032710903137434 ◽

2009 ◽

Vol 42 (14) ◽

pp. 2232-2243 ◽

Cited By ~ 6

Author(s):

Naseem Ahmad Charoo ◽

Mohammad Bashir ◽

Enas Abdalla ◽

Khalid Ibrahim Haj Ali

Keyword(s):

Spectrophotometric Method ◽

Candesartan Cilexetil ◽

Dosage Forms ◽

Dissolution Testing ◽

First Derivative ◽

Tablet Dosage

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Simultaneous Determination of Moxifloxacin and Flavoxate by RP-HPLC and Ecofriendly Derivative Spectrophotometry Methods in Formulations

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph16071196 ◽

2019 ◽

Vol 16 (7) ◽

pp. 1196 ◽

Cited By ~ 6

Author(s):

Mahesh Attimarad ◽

Muhammad Shahzad Chohan ◽

Abdulmalek Ahmed Balgoname

Keyword(s):

Simultaneous Determination ◽

Spectrophotometric Method ◽

High Performance ◽

Internal Standard ◽

Reversed Phase ◽

Simultaneous Estimation ◽

Zero Crossing ◽

Spectrophotometric Methods ◽

First Derivative

Simple, fast, and precise reversed-phase (RP)-high-performance liquid chromatography (HPLC) and two ecofriendly spectrophotometric methods were established and validated for the simultaneous determination of moxifloxacin HCl (MOX) and flavoxate HCl (FLX) in formulations. Chromatographic methods involve the separation of two analytes using an Agilent Zorbax SB C18 HPLC column (150 mm × 4.6 mm; 5 µm) and a mobile phase consisting of phosphate buffer (50 mM; pH 5): methanol: acetonitrile in a proportion of 50:20:30 v/v, respectively. Valsartan was used as an internal standard. Analytes were monitored by measuring the absorbance of elute at 299 nm for MOX and 250 nm for FLX and valsartan. Two environmentally friendly spectrophotometric (first derivative and ratio first derivative) methods were also developed using water as a solvent. For the derivative spectrophotometric determination of MOX and FLX, a zero-crossing technique was adopted. The wavelengths selected for MOX and FLX were −304.0 nm and −331.8 nm for the first derivative spectrophotometric method and 358.4 nm and −334.1 nm for the ratio first-derivative spectrophotometric method, respectively. All methods were successfully validated, as per the International Conference on Harmonization(ICH) guidelines, and all parameters were well within acceptable ranges. The proposed analytical methods were successfully utilized for the simultaneous estimation of MOX and FLX in formulations.

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High-Performance Liquid Chromatographic and First Derivative of the Ratio Spectrophotometric Determination of Amlodipine and Valsartan in Their Binary Mixtures

Journal of AOAC International ◽

10.1093/jaoac/93.3.882 ◽

2010 ◽

Vol 93 (3) ◽

pp. 882-890 ◽

Cited By ~ 9

Author(s):

Dilek Kul ◽

Burcu Dogan-Topal ◽

Tugba Kutucu ◽

Bengi Uslu ◽

Sibel A Ozkan

Keyword(s):

Spectrophotometric Method ◽

High Performance ◽

High Performance Liquid Chromatographic ◽

Hplc Method ◽

Time Saving ◽

Pharmaceutical Dosage Form ◽

First Derivative ◽

Significant Difference ◽

Long Acting

Abstract Amlodipine besylate (AML) is a long-acting calcium channel blocker used as an antihypertensive agent. Valsartan (VAL) is also used to treat hypertension, either alone or in combination with other agents. Two-component mixtures of AML and VAL were analyzed by HPLC and the ratio spectra of the first derivative spectrophotometric technique. The spectrophotometric method depends on the first derivative of the ratio-spectra by measurements of the amplitudes at 234.0 nm for VAL and 351.0 nm for AML. Calibration graphs were established for 0.520 g/mL AML and 132 g/mL VAL using the ratio spectra of the first derivative spectrophotometric method. In the HPLC method, an ACE 5 C18 (4.6 150 mm, 5 m) RP column at 30C with the mobile phase methanolacetonitrileNaH2PO4H2O buffer, including 5 mL/L triethylamine and adjusted to pH 3.0 (42 + 18 + 40, v/v/v) at 2.0 mL/min flow rate was used to separate both compounds with detection at 254.0 nm. Linearity was obtained in the concentration range of 0.5500 g/mL for AML and 5.0900 g/mL for VAL. The proposed methods have been extensively validated. These methods allow a number of cost- and time-saving benefits. They were successfully applied to the determination of AML and VAL in synthetic mixtures and in a pharmaceutical dosage form. There was no significant difference between the performance of the proposed methods regarding the mean and SD values. The proposed methods are simple, rapid, and suitable for QC applications.

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Development and Validation of Column High-Performance Liquid Chromatographic and Derivative Spectrophotometric Methods for Determination of Levofloxacin and Ornidazole in Combined Dosage Forms

Journal of AOAC International ◽

10.1093/jaoac/91.4.756 ◽

2008 ◽

Vol 91 (4) ◽

pp. 756-761 ◽

Cited By ~ 3

Author(s):

Satish A Patel ◽

Arun M Prajapati ◽

Paresh U Patel ◽

Natubhai J Patel ◽

Jayesh B Vaghmasi

Keyword(s):

Spectrophotometric Method ◽

High Performance ◽

High Performance Liquid Chromatographic ◽

Dosage Forms ◽

Zero Crossing ◽

Spectrophotometric Methods ◽

First Derivative ◽

Rp Hplc ◽

Liquid Chromatographic

Abstract The manuscript describes validated reversed-phase column high-performance liquid chromatographic (RP-HPLC) and first-derivative UV spectrophotometric methods for the estimation of levofloxacin (LFX) and ornidazole (ORNI) in combined dosage forms. The RP-HPLC separation was achieved on a Phenomenex C18 column (250 mm 4.6 mm id, 5 m) using KH2PO4 buffer (pH 6.8)methanolacetonitrile (70 + 15 + 15, v/v/v) mobile phase at a flow rate of 1.5 mL/min and ambient temperature (25 2<sup/>C). Quantification was achieved with photodiode array detection at 295 nm over the concentration range of 110 g/mL for both LFX and ORNI, with mean recovery of 101.7 0.23 and 99.23 1.57, respectively, by the RP-HPLC method. The derivative spectrophotometric method was based on the determination of both the drugs at their respective zero crossing point (ZCP). The first-order derivative spectra were obtained at N =1 (scaling factor), = 2.0 nm (wavelength interval), and the determinations were made at 310 nm (ZCP of ORNI) for LFX and 295 nm (ZCP of LFX) for ORNI over the concentration range of 240 g/mL for both LFX and ORNI. Mean recovery was 99.46 0.96 and 100.9 0.72, respectively, by the first-derivative UV spectrophotometric method. Standard and sample solutions were prepared with methanol as the solvent in both of the methods. These methods were found to be simple, accurate, precise, and sensitive and were applicable for the simultaneous determination of LFX and ORNI in combined dosage forms.

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A novel stability-indicating method for determination of a new antidepressant effect of vortioxetine in a pharmaceutical formulation by using RP-HPLC

Future Journal of Pharmaceutical Sciences ◽

10.1186/s43094-020-00135-w ◽

2020 ◽

Vol 6 (1) ◽

Author(s):

Gizem Tiris ◽

Cansu Alver ◽

Nevin Erk

Keyword(s):

Spectrophotometric Method ◽

Limit Of Detection ◽

Internal Standard ◽

Chemical Oxidation ◽

Hplc Method ◽

Dihydrogen Phosphate ◽

Stability Indicating ◽

First Derivative ◽

Rp Hplc

Abstract Background A novel rapid, accurate, and stability-indicating reversed-phase high performance liquid chromatographic (RP-HPLC) and first derivative spectrophotometric determination were explained for the assay of vortioxetine (VRT) in bulk and pharmaceutical formulations. For RP-HPLC method, optimal separation and determination of VRT were achieved with a Waters Symmetry C18, (100 × 4.6 mm, 3.5 μm) analytical column using a mobile phase consisting of methanol:0.05 M potassium dihydrogen phosphate (pH:3.0 ± 0.05) (30:70, v/v) in isocratic mode with flow rate of 1.3 mL min−1. Injection volume was 20 μL. The maximum absorption wavelength of VRT is 225.0 nm; hence, 225.0 nm was studied as the detection wavelength and column at 50 °C temperature. The caffeine was used as the internal standard (IS). On the other hand, the first derivative spectrophotometric method for the analysis of vortioxetine was performed by measuring the amplitude at 251.7 and 272.6 nm. Result The HPLC method was found to be linear in the concentration ranges of 10.0-70.0 μg mL−1 with the coefficient value R2 of 0.9998, and the mean recovery value was 100.7%. Further stability studies were done through exposure of the analyte solutions to various stress conditions: acid, alkali hydrolysis, chemical oxidation, and exposure to UV radiation. For the first derivative spectrophotometric method, linearity was observed in the concentration range 6.0-30.0 μg mL−1 (for 237.7 nm R2 = 0.9999 and for 257.2 nm R2 = 0.9997). Conclusion The methods were validated in accordance with ICH guidelines with respect to linearity, accuracy, specificity, limit of detection, precision, and limit of quantification.

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Determination of Glibenclamide By Analytical Spectrophotometry

JOURNAL OF ADVANCES IN CHEMISTRY ◽

10.24297/jac.v18i.8963 ◽

2021 ◽

Vol 18 ◽

pp. 40-48

Author(s):

Saad Antakli ◽

Leon Nejem ◽

Monzer Alraii

Keyword(s):

Quality Control ◽

Regression Analysis ◽

Spectrophotometric Method ◽

Limit Of Detection ◽

Correlation Coefficients ◽

Extraction Process ◽

Metformin Hydrochloride ◽

Limit Of Quantification ◽

First Derivative

Simple and rapid spectrophotometric method was developed and applied to determine Glibenclamide (GB) by zero spectrophotometric method and first derivative spectrophotometric method for determining of (GB) in the presence of Metformin hydrochloride (MET). Zero spectrophotometric (ZS) method was applied for the determination of (GB) at λmax = 300 nm. Linearity range was (4 – 360) μg/mL. Regression analysis showed a good correlation coefficients R2 = 0.99993. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.65 μg/mL and 2.31 μg/mL, respectively. First derivative spectrophotometric (1DS) method was applied for the determination of (GB) in the presence (MET). (GB) was determined at 317 nm (1D317). Linearity ranges were (4 – 240) μg/mL for (GB). Regression analysis showed a good correlation coefficients R2 = 0.999914. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.60 μg/mL and 1.83 μg/mL for (GB). The proposed zero spectrophotometry method was applied to analysis individual (GB), and the derivative (1D317) method was applied to analysis (GB) individually or combined with (MET) in Syrian trademark drugs. The proposed method is simple, direct, sensitive and do not require any extraction process. Thus, this method could be readily applicable for the quality control and routine analysis.

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