Letrozole Therapy for Obstructive Azoospermic Men before in vitro Fertilization (IVF) treatment with Percutaneous Epididymal Sperm Aspiration

ABSTRACT Objective To evaluate the incidence of Y-chromosome microdeletions in individuals born from vasectomized fathers who underwent vasectomy reversal or in vitro fertilization with sperm retrieval by epididymal aspiration (percutaneous epididymal sperm aspiration). Methods A case-control study comprising male children of couples in which the man had been previously vasectomized and chose vasectomy reversal (n=31) or in vitro fertilization with sperm retrieval by percutaneous epididymal sperm aspiration (n=30) to conceive new children, and a Control Group of male children of fertile men who had programmed vasectomies (n=60). Y-chromosome microdeletions research was performed by polymerase chain reaction on fathers and children, evaluating 20 regions of the chromosome. Results The results showed no Y-chromosome microdeletions in any of the studied subjects. The incidence of Y-chromosome microdeletions in individuals born from vasectomized fathers who underwent vasectomy reversal or in vitro fertilization with spermatozoa recovered by percutaneous epididymal sperm aspiration did not differ between the groups, and there was no difference between control subjects born from natural pregnancies or population incidence in fertile men. Conclusion We found no association considering microdeletions in the azoospermia factor region of the Y chromosome and assisted reproduction. We also found no correlation between these Y-chromosome microdeletions and vasectomies, which suggests that the assisted reproduction techniques do not increase the incidence of Y-chromosome microdeletions.

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Office-based minimally invasive epididymal sperm aspiration (MIESA) for obstructive azoospermia (OA) results in high quality cryopreserved samples and excellent in vitro fertilization (IVF) outcomes

Fertility and Sterility ◽

10.1016/j.fertnstert.2017.07.903 ◽

2017 ◽

Vol 108 (3) ◽

pp. e305-e306

Author(s):

R.C. Owen ◽

J.E. Gray ◽

M.A. Fritz ◽

C.F. Boylan ◽

R.M. Coward

Keyword(s):

In Vitro Fertilization ◽

Minimally Invasive ◽

Obstructive Azoospermia ◽

Epididymal Sperm ◽

High Quality ◽

Vitro Fertilization ◽

Ivf Outcomes ◽

Sperm Aspiration

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In Vitro Fertilization Outcome After Intracytoplasmic Sperm Injection with Fresh and with Frozen-Thawed Epididymal Spermatozoa

KnE Medicine ◽

10.18502/kme.v1i1.533 ◽

2016 ◽

Vol 1 (1) ◽

Author(s):

Hilma Putri Lubis

Keyword(s):

In Vitro Fertilization ◽

Intracytoplasmic Sperm Injection ◽

Oocyte Retrieval ◽

Epididymal Sperm ◽

Vitro Fertilization ◽

Fertilization Rates ◽

Sperm Aspiration ◽

Significant Difference ◽

Epididymal Spermatozoa

IntroductionTesticular epididymal sperm aspiration (TESA) is one of the method to retrieve sperm from the testes in men with azoospermia. The aim of the study is to compare the In vitro fertilization (IVF) outcome of intracytoplasmic sperm injection (ICSI)-ET cycles with fresh testicular epididymal spermatozoa obtained on the same day with oocyte retrieval and with frozen-thawed testicular epididymal spermatozoa.Material & MethodsA retrospective comparative analysis of patients who underwent fresh TESA and frozen-thawed TESA in ICSI-ET cycles from January 2012 to December 2014 in Halim Fertility Center was done. Fresh testicular epididymal sperm aspiration (fresh TESA) was performed on the same day with oocyte retrieval in 28 cycles and the frozen-thawed testicular epididymal sperm aspiration (frozen-thawed TESA) was used in 30 cycles. ResultsThe two groups were comparable in terms of the ages of male and female patients, etiology of infertility and duration of infertility. Fertilization rates in fresh TESA group were 53,5% and in frozen-thawed TESA group, fertilization rates were 50%. There was no statistically significant difference between the groups. Clinical pregnancy rates in fresh TESA group were 35,7% and in frozen-thawed TESA group, clinical pregnancy rates were 26,7% and statistically there was no significant difference between the groups.ConclusionThere is no significant difference in the in vitro fertilization outcome of intracytoplasmic sperm injection (ICSI)-ET cycles between fresh TESA and frozen-thawed TESA .

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