scholarly journals Incidence of Y-chromosome microdeletions in children whose fathers underwent vasectomy reversal or in vitro fertilization with epididymal sperm aspiration: a case-control study

2016 ◽  
Vol 14 (4) ◽  
pp. 534-540 ◽  
Author(s):  
Milton Ghirelli-Filho ◽  
◽  
Patricia Leme de Marchi ◽  
Fernanda Abani Mafra ◽  
Viviane Cavalcanti ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Y Chromosome ◽  
Epididymal Sperm ◽  
Sperm Retrieval ◽  
Vitro Fertilization ◽  
Y Chromosome Microdeletions ◽  
Sperm Aspiration ◽  
Vasectomy Reversal ◽  
Control Study

ABSTRACT Objective To evaluate the incidence of Y-chromosome microdeletions in individuals born from vasectomized fathers who underwent vasectomy reversal or in vitro fertilization with sperm retrieval by epididymal aspiration (percutaneous epididymal sperm aspiration). Methods A case-control study comprising male children of couples in which the man had been previously vasectomized and chose vasectomy reversal (n=31) or in vitro fertilization with sperm retrieval by percutaneous epididymal sperm aspiration (n=30) to conceive new children, and a Control Group of male children of fertile men who had programmed vasectomies (n=60). Y-chromosome microdeletions research was performed by polymerase chain reaction on fathers and children, evaluating 20 regions of the chromosome. Results The results showed no Y-chromosome microdeletions in any of the studied subjects. The incidence of Y-chromosome microdeletions in individuals born from vasectomized fathers who underwent vasectomy reversal or in vitro fertilization with spermatozoa recovered by percutaneous epididymal sperm aspiration did not differ between the groups, and there was no difference between control subjects born from natural pregnancies or population incidence in fertile men. Conclusion We found no association considering microdeletions in the azoospermia factor region of the Y chromosome and assisted reproduction. We also found no correlation between these Y-chromosome microdeletions and vasectomies, which suggests that the assisted reproduction techniques do not increase the incidence of Y-chromosome microdeletions.

scholarly journals In Vitro Fertilization Outcome After Intracytoplasmic Sperm Injection with Fresh and with Frozen-Thawed Epididymal Spermatozoa

KnE Medicine ◽  
2016 ◽  
Vol 1 (1) ◽  
Author(s):  
Hilma Putri Lubis
Keyword(s):  
In Vitro Fertilization ◽  
Intracytoplasmic Sperm Injection ◽  
Oocyte Retrieval ◽  
Epididymal Sperm ◽  
Vitro Fertilization ◽  
Fertilization Rates ◽  
Sperm Aspiration ◽  
Significant Difference ◽  
Epididymal Spermatozoa

<p><strong>Introduction</strong></p><p>Testicular epididymal sperm aspiration (TESA) is one of the method  to retrieve sperm from the testes in men with azoospermia. The aim of the study is to compare the In vitro fertilization (IVF) outcome of intracytoplasmic sperm injection (ICSI)-ET cycles with fresh testicular epididymal spermatozoa obtained on the same day with  oocyte retrieval and with frozen-thawed testicular epididymal spermatozoa.</p><p><strong>Material &amp; Methods</strong></p><p>A retrospective comparative analysis of  patients who underwent fresh TESA and frozen-thawed TESA in ICSI-ET cycles from January 2012 to December 2014 in Halim Fertility Center was done. Fresh testicular epididymal sperm aspiration (fresh TESA) was performed on the same day with oocyte retrieval in 28 cycles and the frozen-thawed testicular epididymal sperm aspiration (frozen-thawed TESA) was used in 30 cycles.  </p><p><strong>Results</strong></p><p>The two groups were comparable in terms of the ages of male and female patients, etiology of infertility and duration of infertility. Fertilization rates in fresh TESA group were 53,5% and in frozen-thawed TESA group, fertilization rates were 50%. There was no statistically significant difference between the groups. Clinical pregnancy rates in fresh TESA group were 35,7%  and in frozen-thawed TESA group, clinical pregnancy rates were 26,7% and statistically there was no significant difference between the groups.</p><p><strong>Conclusion</strong></p>There is no significant difference in the in vitro fertilization outcome of intracytoplasmic sperm injection (ICSI)-ET cycles between fresh TESA and frozen-thawed TESA .

Use of epididymal sperm for assisted reproduction in men with acquired, irreparable obstructive azoospermia

1995 ◽  
Vol 7 (4) ◽  
pp. 841 ◽  
Author(s):  
P Patrizio ◽  
T Ord ◽  
JP Balmaceda ◽  
RH Asch
Keyword(s):  
Vas Deferens ◽  
Sperm Count ◽  
Fertilization Rate ◽  
Obstructive Azoospermia ◽  
Epididymal Sperm ◽  
Vitro Fertilization ◽  
Sperm Aspiration ◽  
Significant Difference ◽  
Vasectomy Reversal

Microsurgical epididymal sperm aspiration (MESA) and in vitro fertilization (IVF) is primarily offered to men with congenital absence of the vas deferens (CAVD). However, the IVF capacity of these epididymal sperm is low ( < 15%) and unpredictable. In this study, IVF and intracytoplasmic sperm injection (ICSI) results in patients with non-congenital, irreparable obstructive azoospermia were analysed. Thirty-three patients were evaluated for a total of 37 cycles of MESA and IVF. Most had obstruction secondary to failed vasectomy reversal and to epididymal blockage. The overall fertilization rate was 30% with regular IVF and 26% with ICSI, and six clinical pregnancies were obtained. Both rates are significantly higher than the IVF rate previously reported with sperm from men with CAVD (13%, P < 0.00001). In men with non-congenital obstructive azoospermia, a significant difference was found in the average sperm count (56.9 x 10(6) v. 12.3 x 10(6), P < 0.04) and total motile count (16.6 x 10(6) v. 1.6 x 10(6), P < 0.01) respectively for patients who achieved IVF and those who did not. It is concluded that (a) the real IVF capacity of human epididymal sperm is 30%, or 42%, if calculated only for patients who achieved fertilization, (b) this higher rate is an indirect support of the hypothesis that sperm from men with CAVD have intrinsic biochemical defects, related to cystic fibrosis mutations, responsible for their low and unpredictable IVF rate, and (c) MESA and IVF can be offered at the same time or as an alternative to patients requesting vasectomy reversal.

scholarly journals Letrozole Therapy for Obstructive Azoospermic Men before in vitro Fertilization (IVF) treatment with Percutaneous Epididymal Sperm Aspiration

2015 ◽  
Vol 19 (4) ◽  
Author(s):  
Mauro Bibancos ◽  
Melissa Cavagnoli ◽  
Tatiana C. S. Bonetti ◽  
Erika Semaco ◽  
Eduardo L. A. Motta ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Epididymal Sperm ◽  
Vitro Fertilization ◽  
Sperm Aspiration

A simplified method for processing sperm obtained during microsurgical epididymal sperm aspiration (MESA) for use in in vitro fertilization

1992 ◽  
Vol 9 (6) ◽  
pp. 580-583 ◽  
Author(s):  
Anil K. Dubey ◽  
Robert D. Oates ◽  
Vito R. S. Cardone ◽  
Nancy A. Harrington ◽  
D. Robert A. McInnes
Keyword(s):  
In Vitro Fertilization ◽  
Epididymal Sperm ◽  
Vitro Fertilization ◽  
Sperm Aspiration ◽  
Simplified Method

scholarly journals The best infertility treatment for vasectomized men: assisted reproduction or vasectomy reversal?

2004 ◽  
Vol 59 (5) ◽  
pp. 312-315 ◽  
Author(s):  
Fábio Firmbach Pasqualotto ◽  
Antônio Marmo Lucon ◽  
Bernardo Passos Sobreiro ◽  
Eleonora Bedin Pasqualotto ◽  
Sami Arap
Keyword(s):  
Surgical Treatment ◽  
In Vitro Fertilization ◽  
Intracytoplasmic Sperm Injection ◽  
Reproductive Tract ◽  
Cost Effective ◽  
Sperm Retrieval ◽  
Microsurgical Reconstruction ◽  
Vitro Fertilization ◽  
Vasectomy Reversal

In men with prior vasectomy, microsurgical reconstruction of the reproductive tract is more cost-effective than sperm retrieval with in vitro fertilization and intracytoplasmic sperm injection if the obstructive interval is less than 15 years and no female fertility risk factors are present. If epididymal obstruction is detected or advanced female age is present, the decision to use either microsurgical reconstruction or sperm retrieval with in vitro fertilization and intracytoplasmic sperm injection should be individualized. Sperm retrieval with in vitro fertilization and intracytoplasmic sperm injection is preferred to surgical treatment when female factors requiring in vitro fertilization are present or when the chance for success with sperm retrieval and intracytoplasmic sperm injection exceeds the chance for success with surgical treatment.

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