Optimization of fluorescent antibody techniques for demonstration of foot-and-mouth disease virus in bovine tongue epithelium and dorsal soft palate

Sheep infected with FMDV strains of different epizootiological origin developed a carrier state which persisted in the majority of animals for 1–5 months.The sites of virus persistence and multiplication in the convalescent animal were identified by titration of suspensions of mucosae and epithelia taken post mortem. Virus was recovered most frequently and in highest titre from the tonsillar area and less frequently from the pharynx and dorsal surface of the soft palate. No virus was found in samples taken from the nasal passages, the trachea or the rumen.

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STANDARDIZATION OF MULTIPLEX REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION AND TYPING OF FOOT-AND-MOUTH DISEASE VIRUS PREVALENT IN BANGLADESH

Bangladesh Journal of Veterinary Medicine ◽

10.3329/bjvm.v8i2.11199 ◽

2012 ◽

Vol 8 (2) ◽

pp. 149-155 ◽

Cited By ~ 1

Author(s):

MA Zinnah ◽

MT Islam ◽

MM Rahman ◽

MT Hossain ◽

MA Zinnah ◽

...

Keyword(s):

Disease Virus ◽

Research Work ◽

Foot And Mouth Disease ◽

Mouth Disease ◽

Economic Losses ◽

Fmdv Serotypes ◽

Mouth Disease Virus ◽

Field Samples ◽

Foot And Mouth ◽

Tongue Epithelium

Foot-and-mouth disease (FMD) is a devastating viral disease of cattle that causes severe economic losses in terms of loss of production and calf mortality in Bangladesh. Despite of regular vaccination, outbreak of the disease has become a regular event throughout the country every year. Determination of prevailing serotypes of the causal agent foot-and-mouth disease virus (FMDV) is now crucial need for strategic vaccination programme. The present research work was aimed to standardize a multiplex RT-PCR assay typing of foot-and-mouth disease virus serotypes prevalent among cattle population of Bangladesh. Uniplex and multiplex RT-PCRs were successfully developed and standardized using the extracted RNA of reference FMDV (Type A, O and Asia 1) following adjustment of the concentration of the viral RNA of each serotype, volume of reaction mixture and thermal profile. The mPCR was evaluated on 82 field samples (vesicular fluid, tongue epithelium and tissue from inter-digital space) of the years 2007 and 2008. Of the 82 field samples, 56 (68.29%) were found positive for FMDV. The mPCR successfully differentiated single as well as dual serotypes infection. The serotypes A, O and Asia 1 were confirmed in the samples of the year 2007 and only serotype O in samples of the year 2008. Higher detection rate was found in vesicular fluid (100%) followed by tongue epithelium (79.66%). It may be concluded that the MRT-PCR standardized in this study could be used for detection and differentiation of FMDV serotypes using field samples.DOI = http://dx.doi.org/10.3329/bjvm.v8i2.11199 Bangl. J. Vet. Med. (2010). 8 (2) : 149-155

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Proteogenomics Uncovers Critical Elements of Host Response in Bovine Soft Palate Epithelial Cells Following In Vitro Infection with Foot-And-Mouth Disease Virus

Viruses ◽

10.3390/v11010053 ◽

2019 ◽

Vol 11 (1) ◽

pp. 53 ◽

Cited By ~ 3

Author(s):

Florian Pfaff ◽

Sara Hägglund ◽

Martina Zoli ◽

Sandra Blaise-Boisseau ◽

Eve Laloy ◽

...

Keyword(s):

Epithelial Cells ◽

Antiviral Activity ◽

Disease Virus ◽

Soft Palate ◽

Foot And Mouth Disease ◽

Mouth Disease ◽

Transcriptional Responses ◽

Mouth Disease Virus ◽

Foot And Mouth

Foot-and-mouth disease (FMD) is the most devastating disease of cloven-hoofed livestock, with a crippling economic burden in endemic areas and immense costs associated with outbreaks in free countries. Foot-and-mouth disease virus (FMDV), a picornavirus, will spread rapidly in naïve populations, reaching morbidity rates of up to 100% in cattle. Even after recovery, over 50% of cattle remain subclinically infected and infectious virus can be recovered from the nasopharynx. The pathogen and host factors that contribute to FMDV persistence are currently not understood. Using for the first time primary bovine soft palate multilayers in combination with proteogenomics, we analyzed the transcriptional responses during acute and persistent FMDV infection. During the acute phase viral RNA and protein was detectable in large quantities and in response hundreds of interferon-stimulated genes (ISG) were overexpressed, mediating antiviral activity and apoptosis. Although the number of pro-apoptotic ISGs and the extent of their regulation decreased during persistence, some ISGs with antiviral activity were still highly expressed at that stage. This indicates a long-lasting but ultimately ineffective stimulation of ISGs during FMDV persistence. Furthermore, downregulation of relevant genes suggests an interference with the extracellular matrix that may contribute to the skewed virus-host equilibrium in soft palate epithelial cells.

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