Isolation of Entomopathogenic Nematode Heterorhabditis indica from Hauz Khas Lake Area in Delhi

2021 ◽  
Vol 51 (1) ◽  
pp. 85-89
Author(s):  
Razia Sultana ◽  
Vishal Singh Somvanshi
Keyword(s):  
Entomopathogenic Nematode ◽  
Lake Area ◽  
Heterorhabditis Indica

Virulence of two entomopathogenic nematode species, Steinernema sp. (strain PQ16) and Heterorhabditis indica (strain KT3987), to nymphs of the coffee cicada Dundubia nagarasingna

Nematology ◽  
2019 ◽  
Vol 21 (1) ◽  
pp. 35-44 ◽  
Author(s):  
Chau N. Nguyen ◽  
Anh T. Do ◽  
Phap Q. Trinh ◽  
Phuc K. Hoang
Keyword(s):  
Biological Control ◽  
Entomopathogenic Nematodes ◽  
Entomopathogenic Nematode ◽  
Nematode Species ◽  
Mortality Rates ◽  
Coffee Plantations ◽  
Heterorhabditis Indica ◽  
Inoculation Dose ◽  
Pot Trial

Summary The virulence and efficacy of two species of entomopathogenic nematodes, Steinernema sp. (strain PQ16) and Heterorhabditis indica (strain KT3987), against nymphs of the coffee cicada, Dundubia nagarasingna, was evaluated under laboratory and glasshouse conditions. The highest mortality rates of coffee cicada nymphs caused by these two nematode strains were 93.5 and 100%, respectively, at an inoculation dose of 600 infective juveniles (IJ) nymph−1. The virulence (LC50) was established as 137.5 IJ and 149.1 IJ for strains S-PQ16 and H-KT3987, respectively. The highest IJ yields of these nematode strains were 66 × 103 IJ (for S-PQ16) and 134.4 × 103 IJ (for H-KT3987) at a dose of 500 IJ nymph−1. The efficacies of the two nematode strains to coffee cicadas at treated dose of 60 × 103 IJ pot−1 were 84.4 and 88.9% after 30 days, higher than the efficacies at treated dose of 40 × 103 IJ pot−1. The number of IJ in 250 ml of soil at 10, 20 and 30 days after treatment, increased from 0.38 × 103 to 4.80 × 103 IJ in soil treated with a dose of 40 × 103 IJ and from 0.66 × 103 to 5.02 × 103 IJ in soil treated with a dose of 60 × 103 IJ (for S-PQ16). Similarly, for H-KT3987 the number of IJ increased from 0.43 × 103 to 8.99 × 103 IJ and from 0.62 × 103 to 9.64 × 103 IJ, at the respective doses. Based on results of a pot trial from glasshouse modelling, an IJ application dosage for biological control of coffee cicada nymphs in coffee plantations was proposed.

scholarly journals Assessing the Pathogenicity of Two Bacteria Isolated from the Entomopathogenic Nematode Heterorhabditis indica against Galleria mellonella and Some Pest Insects

Insects ◽  
2019 ◽  
Vol 10 (3) ◽  
pp. 83
Author(s):  
Rosalba Salgado-Morales ◽  
Fernando Martínez-Ocampo ◽  
Verónica Obregón-Barboza ◽  
Kathia Vilchis-Martínez ◽  
Alfredo Jiménez-Pérez ◽  
...  
Keyword(s):  
16S Rrna ◽  
Galleria Mellonella ◽  
De Novo ◽  
Entomopathogenic Nematode ◽  
23S Rrna ◽  
Its2 Region ◽  
Rrna Gene ◽  
28S Rrna ◽  
Heterorhabditis Indica ◽  
Insecticide Activity

The entomopathogenic nematodes Heterorhabditis are parasites of insects and are associated with mutualist symbiosis enterobacteria of the genus Photorhabdus; these bacteria are lethal to their host insects. Heterorhabditis indica MOR03 was isolated from sugarcane soil in Morelos state, Mexico. The molecular identification of the nematode was confirmed using sequences of the ITS1-5.8S-ITS2 region and the D2/D3 expansion segment of the 28S rRNA gene. In addition, two bacteria HIM3 and NA04 strains were isolated from the entomopathogenic nematode. The genomes of both bacteria were sequenced and assembled de novo. Phylogenetic analysis was confirmed by concatenated gene sequence datasets as Photorhabdus luminescens HIM3 (16S rRNA, 23S rRNA, dnaN, gyrA, and gyrB genes) and Pseudomonas aeruginosa NA04 (16S rRNA, 23S rRNA and gyrB genes). H. indica MOR03 infects Galleria mellonella, Tenebrio molitor, Heliothis subflexa, and Diatraea magnifactella larvae with LC50 values of 1.4, 23.5, 13.7, and 21.7 IJs/cm2, respectively, at 48 h. These bacteria are pathogenic to various insects and have high injectable insecticide activity at 24 h.

scholarly journals Physiological alterations in Bradybaena similaris (Stylommatophora: Bradybaenidae) induced by the entomopathogenic nematode Heterorhabditis indica (Rhabditida: Heterorhabditidae) strain LPP1

2014 ◽  
Vol 139 ◽  
pp. 12-18 ◽  
Author(s):  
Victor Menezes Tunholi ◽  
Caio Oliveira Monteiro ◽  
Lidiane Cristina da Silva ◽  
Claudia de Melo Dolinski ◽  
Marcos Antônio José dos Santos ◽  
...  
Keyword(s):  
Entomopathogenic Nematode ◽  
Heterorhabditis Indica ◽  
Physiological Alterations

Pathogenicity of four entomopathogenic nematode strains against Asian corn borer, Ostrinia furnacalis (Guenée), in Vietnam

Nematology ◽  
2018 ◽  
Vol 20 (8) ◽  
pp. 729-736
Author(s):  
Chau N. Nguyen ◽  
Anh T. Do ◽  
Phuc K. Hoang ◽  
Lam X. Truong
Keyword(s):  
Biological Control ◽  
Entomopathogenic Nematode ◽  
Reproductive Capacity ◽  
Ostrinia Furnacalis ◽  
Corn Borer ◽  
Heterorhabditis Indica ◽  
Low Concentrations ◽  
Asian Corn Borer ◽  
High Virulence ◽  
High Degree

Summary The virulence and reproductive capacity of four indigenous entomopathogenic nematode (EPN) strains, S-PQ16 (Steinernema sp. PQ16), S-TX1 (Steinernema sangi TX1), S-DL13 (S. siamkayai DL13) and H-NT3 (Heterorhabditis indica NT3), against the last instar larvae of Asian corn borer (ACB), was evaluated. With moderately low concentrations of infective juveniles (IJ) from 10 to 100 IJ insect−1, the ACB larvae were found to be susceptible to all four EPN nematode strains after exposure for 24, 48, 72, 96 and 120 h. The 100% mortality of ACB larvae by these indigenous EPN strains occurred after 96-120 h of exposure. The low LC50 values (between 21.7 and 27.9 IJ) indicated the high virulence of these four EPN strains to ACB larvae. The highest yield of IJ produced inside the insect cadavers for S-PQ16, S-TX1, S-DL13 and H-NT3 was 215.0 ± 6.5 × 103, 83.1 ± 4.9 × 103, 67.8 ± 7.2 × 103 and 225.2 ± 6.4 × 103 IJ, respectively, with low inoculated concentrations of between 30-50 IJ. The results, showing a high degree of virulence and high reproduction capacity, suggest that these indigenous EPN strains have great potential for biological control of the Asian corn borer.

Sign in / Sign up

Export Citation Format

Share Document