Electrophoretic profile of serum proteins in dromedary camels

Background: Electrophoresis of serum proteins is an orientation examination routinely used in clinical practice. For a few years, agarose gel electrophoresis has tended to be replaced with capillary electrophoresis owing to an increase in the accuracy of results. However, this technique is uncommon and is not widely used in Cameroon. Objectives: The research aimed at studying the electrophoretic profile of serum proteins using capillary technique among patients attending the Douala General Hospital, Cameroon. Methods: Capillary electrophoresis was used to carry out tests on blood samples from any inpatients and outpatients and fasting for 8-12 hours. Capillary electrophoresis of serum samples was used for the separation of proteins into six fractions and the total protidemia of each serum samples was determined using the Biuret method. Results were interpreted by observing the shape of curves and quantitative variations in each fraction of the different serum proteins. Results: A total of 311 patients participated in the study. The sampled population aged 50±18 years on average and consisted of 55.3% men and 44.7% women. All capillary electrophoresis profiles presented six protein fractions, namely, albumin, alpha (1 and 2), beta (1 and 2) and gamma globulins. Pathological disorders were diagnosed in 290 patients and 21 patients had normal results. Inflammatory syndromes accounted for 63.34% and monoclonal gammopathies for 10.29% the main pathological disorder identified. Conclusion: Capillary electrophoresis provides a more precise identification of biological syndromes and clear distinction of the six fractions of each protein. Monoclonal profiles and inflammatory syndromes were well detected. A prevalence of 10.29% was determined for gammopathies.

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Fine Structural Alterations in Thyroid Follicular Cells (FC) and Changes in Serum Thyroxine Produced by Feeding Polychlorinated Biphenyl (PCB) to Rats

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100079929 ◽

1977 ◽

Vol 35 ◽

pp. 498-499

Author(s):

W.T. Collins ◽

Charles C. Capen ◽

Louis Kasza

Keyword(s):

Feed Efficiency ◽

Polychlorinated Biphenyl ◽

Serum Proteins ◽

Skin Lesions ◽

Hepatic Necrosis ◽

Ultrastructural Changes ◽

Lymphoid Tissues ◽

Heat Transfer Fluids ◽

Thyroid Follicular Cells ◽

Peripheral Metabolism

The widespread contamination of the environment with PCB, a compound used extensively by industry in hydraulic and heat transfer fluids as well as plasticizers and solvents in adhesives and sealants, has resulted in detectable tissue levels in a large portion of the human population, domestic animals, and wildlife. Intoxication with PCB produces severe hepatic necrosis, degeneration of lymphoid tissues and kidney, skin lesions, decreased reproductive performance, reduced feed efficiency, and decreased weight gain. PCB also has been reported to reduce the binding of thyroid hormone to serum proteins and enhance the peripheral metabolism of thyroxine with increased excretion of thyroxine-glucuronide in the bile (Bastomsky, Endocrinology 95: 1150-1155, 1974).The objectives of this investigation were (1) to investigate the histopathologic, histochemical, and ultrastructural changes in thyroid FC produced by the acute (4 week) and chronic (12 week) administration of low (50 ppm) and high (500 ppm) doses of PCB to rats, (2) to correlate these alterations to changes in serum immunoreactive thyroxine concentration, and (3) to investigate the persistence of the effects of PCB on the thyroid gland.

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Serum Proteins in Ulcerative Colitis

Gastroenterology ◽

10.1016/s0016-5085(19)35413-7 ◽

1959 ◽

Vol 37 (3) ◽

pp. 263-267 ◽

Cited By ~ 6

Author(s):

Richard O. Bicks ◽

Joseph B. Kirsner ◽

Walter L. Palmer

Keyword(s):

Ulcerative Colitis ◽

Serum Proteins

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Prothrombin Evaluation as Obtained by Kinetics Studies of Antigen-Antibody Reaction in a Laser Nephelometer

Thrombosis and Haemostasis ◽

10.1055/s-0038-1661126 ◽

1984 ◽

Vol 52 (01) ◽

pp. 015-018 ◽

Cited By ~ 3

Author(s):

A Girolami ◽

A Sticchi ◽

R Melizzi ◽

L Saggin ◽

G Ruzza

Keyword(s):

Liver Cirrhosis ◽

Cirrhotic Patient ◽

Maximum Rate ◽

Serum Proteins ◽

Clotting Factors ◽

Kinetics Studies ◽

Maximum Value ◽

Time Required ◽

Antigen Antibody ◽

Kinetics Of

SummaryLaser nephelometry is a technique which allows the evaluation of the concentration of several serum proteins and clotting factors. By means of this technique it is also possible to study the kinetics of the reaction between antigen and antibody. We studied the kinetics of the reaction between prothrombin and an antiprothrombin antiserum using several prothrombins namely: Prothrombin Padua, prothrombin Molise, which are two congenital dysprothrombinemias, cirrhotic, coumarin or normal prothrombins. Different behaviors in the kinetics of the reactions were shown even when the concentration of prothrombins was about the same in all plasma tested. These differences were analyzed by means of a computer (Apple II 48 RAM) programmed to solve four unknown equations (Rodbard’s equation). From the data so obtained one can see that when voltages at the beginning and at the end of the reaction are in all cases about the same, a clear difference in the time required to reach half the maximum value of the voltage can still be demonstrated. This parameter, which is expressed in minutes, is longer in coumarin and prothrombin Molise than in controls. On the contrary it is shorter in prothrombin Padua and has about the same value of controls in the cirrhotic patient. Moreover the time at which the maximum rate is obtained is longer in coumarin and prothrombin Molise than in controls and shorter in liver cirrhosis and prothrombin Padua. In conclusion data obtained show that coumarin prothrombin behaves in a different way from cirrhotic prothrombin and also that there is a different behaviour between the two congenital dysprothrombinemias.

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SERUM PROTEINS AND PROTEIN BOUND CARBOHYDRATES IN RATS TREATED WITH GONADOTROPHIC HORMONES

Acta Endocrinologica ◽

10.1530/acta.0.0240061 ◽

1957 ◽

Vol 24 (1) ◽

pp. 61-72 ◽

Cited By ~ 3

Author(s):

Carlo Bernasconi

Keyword(s):

Serum Proteins

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EPIDEMIC THYROIDITIS

Acta Endocrinologica ◽

10.1530/acta.0.0450381 ◽

1964 ◽

Vol 45 (3) ◽

pp. 381-401 ◽

Cited By ~ 15

Author(s):

G. Hintze ◽

P. Fortelius ◽

J. Railo

Keyword(s):

Thyroid Gland ◽

Distribution Pattern ◽

Needle Biopsy ◽

Serum Proteins ◽

Acute Infection ◽

Thyroid Tissue ◽

Subacute Thyroiditis ◽

Present Observation ◽

Upper Respiratory Tract ◽

Endemic Goitre

ABSTRACT A type of subacute thyroiditis occurring epidemically in a factory in Helsinki was observed in 44 cases. In every case the thyroiditis followed an acute infection of the upper respiratory tract. The variation in incidence during one and a half years was in good agreement with that of the acute infection. Since Helsinki is in an endemic goitre region, the fact that the disease was of the migrating type was of great diagnostic importance. In all cases but one, the nodules have persisted. One case of asymptomatic thyroiditis was seen. In the majority of the patients the thyroid gland had been carefully palpated before the thyroiditis occurred, and in all cases the condition was followed up by the same investigator. Special attention was paid to changes in the iodine metabolism, the serum cholesterol, the electrophoretic distribution pattern of the serum proteins, and the circulating thyroid auto-antibodies. In many cases needle biopsy of the thyroid gland was performed. Thyroid function invariably returned to normal with time, although one patient remained in a hypothyroid state for about a year. In no cases were thyroid auto-antibodies found. For the beta-globulin fraction, the electrophoretic distribution pattern of the serum proteins gave values which were still not normalized in any case, and only in two cases was the alpha2-fraction normalized. The needle biopsy, when thyroid tissue was obtained, showed almost the same picture as in endemic goitre, but in some specimens nonspecific inflammatory changes were seen. Prednisolone relieved the symptoms, but did not affect the course of the disease. According to the present observation this type of epidemic thyroiditis would seem to represent a form of nonspecific subacute thyroiditis.

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INTERFERENCE BY SERUM PROTEINS WITH LH RADIOIMMUNOASSAY USING IMMUNOSORBENT

Acta Endocrinologica ◽

10.1530/acta.0.0720235 ◽

1973 ◽

Vol 72 (2) ◽

pp. 235-242 ◽

Cited By ~ 11

Author(s):

A. M. Reuter ◽

J. C. Hendrick ◽

J. Sulon ◽

P. Franchimont

Keyword(s):

Molecular Weight ◽

Human Serum ◽

High Molecular Weight ◽

Serum Proteins ◽

Void Volume ◽

High Molecular Weight Proteins ◽

Serum Fractionation ◽

Covalently Bound

ABSTRACT The percentage of LH* bound to antibodies that have been covalently bound to cellulose is diminished in the presence of LH-free human serum and sera from various species of animals. Serum fractionation studies on Sephadex G 200 show that the greatest interference comes from the proteins eluted in the void volume i. e. the high molecular weight proteins. Specifically, the gamma M globulins and the α2-macroglobulins appear to play an important role, as demonstrated by tests in which these proteins were neutralized by gamma M and α2-macroglobulin antisera.

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TESTOSTERONE BINDING BY HUMAN SERUM PROTEINS

Acta Endocrinologica ◽

10.1530/acta.0.067s105 ◽

1971 ◽

Vol 68 (1_Supplb) ◽

pp. S105 ◽

Cited By ~ 1

Author(s):

O. A. Lea ◽

K. F. Støa

Keyword(s):

Human Serum ◽

Serum Proteins ◽

Human Serum Proteins

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