Effect of Ovsynch Estrus Synchronization Protocol on Certain Mineral Profile of Cyclic Murrah Buffaloes in Summer and Winter Season

The objective of this study was to evaluate the efficacy of delivering reduced doses of hormones via the Bai Hui acupoint in estrus synchronization in goats. A total of 40 goats received intravaginal sponges with medroxyprogesterone acetate for 7 days. The goats were then randomly distributed into 5 treatment: T1 - application of 132.5 ?g of cloprostenol and 300 IU of equine chorionic gonadotropin (eCG), both by intramuscular injection (IM); T2 - application of 39.75 ?g cloprostenol at the Bai Hui acupoint, and 300 IU of eCG by IM; T3 - application of 132.5 ?g of cloprostenol by IM, and 90 IU of eCG at the Bai Hui acupoint; T4 - application of 39.75?g of cloprostenol and 90 UI of eCG, both in Bai Hui and T5 acupuncture: application of 39.75?g of cloprostenol and 90 UI of eCG, both applied in false acupoint. The goats were subjected to an estrus synchronization protocol and monitored for estrus detection, coverage and evaluation of reproductive parameters to detect entry into estrus. The data were subjected to normality tests, followed by appropriate statistical analyses of each variable. There was no significant difference (P > 0.05) in the percentage of animals in estrus (95.00 ± 11.18%), interval between sponge removal and beginning of estrus (49.72 ± 8.93 h), interval between sponge removal and end of estrus (76.84 ± 11.98 h), duration of estrus (27.08 ± 8.68 h), size of the largest follicle (6.82 ± 0.44 mm), interval between sponge removal and ovulation (78.28 ± 10.82 h), time from ovarian onset to estrus (28.52 ± 5.44 h), follicular growth rate (0.86 ± 0.29 mm/day), number of ovulations (1.32 ± 0.23), plasma progesterone concentration at 7 days after ovulation (10.28 ± 1.65 ng.mL-1), and gestation rate at 30 days after the beginning of estrus (75 ± 12.5%). However, the cost of the synchronization protocol per animal was 43.42% lower in treatments 4 and 5 (30% of the doses) than in treatment 1 (100% of the dose). Ovulation and estrus were efficiently synchronized with the use of 39.75 ?g of sodium cloprostenol and 90 UI of eCG, applied at the Bai Hui acupoint or at a false acupoint.

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215SUCCESSFUL OUT-OF-BREEDING SEASON ESTRUS SYNCHRONIZATION FOLLOWED BY FIXED TIME INSEMINATION IN WATERBUFFALO (BUBALUS BUBALIS)

Reproduction Fertility and Development ◽

10.1071/rdv16n1ab215 ◽

2004 ◽

Vol 16 (2) ◽

pp. 229 ◽

Cited By ~ 1

Author(s):

R.M. Züge ◽

U. Rodacki ◽

A.T. Grandi ◽

J.M.J. Aerts ◽

P.E.J. Bols

Keyword(s):

Breeding Season ◽

Buenos Aires ◽

Fixed Time ◽

Estradiol Benzoate ◽

Sao Paulo ◽

Reproductive Efficiency ◽

São Paulo ◽

Estrus Synchronization ◽

The Impact ◽

Synchronization Protocol

The most important barrier to the increase of buffalo productivity is an overall poor reproductive efficiency, characterized by late sexual maturity, seasonal anestrus and long periods of postpartum ovarian inactivity resulting in extended calving intervals and poor expression of estrus behavior (Singh J et al., 2000, Anim. Reprod. Sci. 60–61, 593–604). Buffaloes are seasonal breeders with the highest reproductive activities during winter (short day lengths) and a high frequency of anestrus during the summer months (Singh G et al. 1985, Ind. J. Anim. Res. 19, 57–60). Recent research demonstrated that a combination of progesterone, estradiol benzoate and equine chorionic gonadotropin (eCG) was effective for estrus induction and synchronization in buffalo heifers under Mediterranean conditions (Barile et al. 2001, Livestock Prod. Sci. 68, 283–287). The aim of the present study was to investigate the impact of an estrus synchronization protocol on reproductive efficiency of water buffalo during out of the normal breeding season. A total of six heifers (21 to 23 months of age) and three cows (5, 6 and 18 years of age) were enrolled in an estrus synchronization protocol lasting for 12 days. All animals were kept under tropical conditions in the coastal part of Paraná (Antonina), about 450km south of São Paulo. The experiment was performed in December, 2002, during the Brazilian summer season, when reproductive efficiency of buffaloes is greatly reduced. On the first day of the protocol (Day 0), animals were implanted with an intravaginal device containing 1g of progesterone (DIB, Syntex SA, Buenos Aires, Argentina) and injected with 10mg estradiol benzoate (Estrogin, Famavet, São Paulo, Brazil). On Day 9, the DIB implant was removed and the animals received 150μg (i.m.) of cloprostenol (Prolise, Syntex SA, Buenos Aires, Argentina) and 2500 IU of eCG (Novormon, Syntex SA, Buenos Aires, Argentina). On Day 11, all animals received 1500 IU of hCG (Vetecor, Lab. Calier, Spain). Artificial insemination (AI) was performed on Day 12 using frozen-thawed semen from a bull of proven fertility. Only one AI was performed per heifer/cow. Pregnancies were determined by ultrasound examination at 53 days following AI and confirmed by rectal palpation at 90 days post AI. The use of this estrus synchronization protocol, followed by fixed-time insemination, resulted in four pregnant heifers (66%) and three pregnant cows (100%). Our results demonstrate that buffalo reproduction can be successful during out-of-breeding season when adequate hormonal treatment is used. Additional experiments should be done to validate the protocol.

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Evaluation of split-time artificial insemination following administration of a long or short-term progestin-based estrus synchronization protocol in beef heifers

Theriogenology ◽

10.1016/j.theriogenology.2019.04.011 ◽

2019 ◽

Vol 133 ◽

pp. 179-186 ◽

Cited By ~ 4

Author(s):

E.R. Knickmeyer ◽

J.M. Thomas ◽

J.W.C. Locke ◽

R.C. Bonacker ◽

M.R. Ellersieck ◽

...

Keyword(s):

Artificial Insemination ◽

Estrus Synchronization ◽

Beef Heifers ◽

Short Term ◽

Split Time ◽

Synchronization Protocol

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Study of vaginal microbiota of Holstein cows submitted to an estrus synchronization protocol with the use of intravaginal progesterone device

Research in Veterinary Science ◽

10.1016/j.rvsc.2020.03.027 ◽

2020 ◽

Vol 131 ◽

pp. 1-6

Author(s):

Daniel Lazzari Quadros ◽

Ricardo Zanella ◽

Carlos Bondan ◽

Giovana Ciacci Zanella ◽

Fernanda Luiza Facioli ◽

...

Keyword(s):

Vaginal Microbiota ◽

Holstein Cows ◽

Estrus Synchronization ◽

Synchronization Protocol

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Photoperiod length and the estrus synchronization protocol used before AI affect the twin pregnancy rate in dairy cattle

Theriogenology ◽

10.1016/j.theriogenology.2012.05.014 ◽

2012 ◽

Vol 78 (6) ◽

pp. 1209-1216 ◽

Cited By ~ 25

Author(s):

C. Andreu-Vázquez ◽

I. Garcia-Ispierto ◽

F. López-Gatius

Keyword(s):

Dairy Cattle ◽

Pregnancy Rate ◽

Twin Pregnancy ◽

Estrus Synchronization ◽

Synchronization Protocol

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Effect of modified housing system on physiological parameters of Murrah buffaloes during autumn and winter season

Indian Journal of Animal Research ◽

10.18805/ijar.b-3305 ◽

2017 ◽

Author(s):

Amit Kumar ◽

M. L. Kamboj ◽

Subhash Chandra ◽

Pranay Bharti

Keyword(s):

Skin Temperature ◽

Respiration Rate ◽

Cortisol Level ◽

Rectal Temperature ◽

Plasma Cortisol ◽

Winter Season ◽

Plasma Cortisol Level ◽

Housing System ◽

Murrah Buffaloes ◽

Autumn And Winter

The aim of this study was to assess the effect of modified housing system on physiological parameters like rectal temperature, skin temperature, plasma cortisol level, respiration rate and pulse rate (counts/min) of Murrah buffaloes during autumn and winter seasons. A total of 14 lactating Murrah buffaloes were randomly selected and divided into two groups, on the basis of age, body weight (BW) and parity (P). T1=First group (n=7; BW=544.86kg; P=2.14) of buffaloes was housed under existing loose housing system and T2=Second group (n=7; BW=547.71kg; P=2.28) of buffaloes was housed under modified shed. In the present study, in autumn and winter season the mean rectal temperature were (T1=101.56±0.06 vs. T2=100.94±0.12ºF and T1=100.71±0.10 vs. T2=100.22±0.08ºF), skin temperature were (T1=95.19±0.61 vs. T2=93.01±0.57 ºF and T1=90.27±0.23 vs. T2=89.06±0.27 ºF) and plasma cortisol level (T1=4.04±0.23 vs. T2=3.31±0.21 and T1=3.19±0.12 vs. T2=2.70±0.14 ng/ml) respectively. These parameters were significantly (P less than 0.05) lower in buffaloes housed under T2 as compared to T1 in autumn season, respiration rate (T1=37.39±1.02 vs. T2=30.99±1.21 and T1=26.56±0.84 vs. T2=23.81±0.66 counts/min.) was significantly lower in autumn (P less than 0.01) and winter season (P less than 0.0) and pulse rate (T1=60.91±1.17 vs. T2=52.52±1.44 and T1=55.01±0.52 vs. T2=51.27±0.53 counts/min) was significantly (P less than 0.01) lower in buffaloes housed under T2 as compared to T1 in both seasons. The Murrah buffaloes housed under modified shed were improved physiological reactions during the autumn and winter seasons.

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