Optimized Cell Survival and Seeding Efficiency for Craniofacial Tissue Engineering Using Clinical Stem Cell Therapy

AbstractCell cryopreservation has evolved as an important technology required for supporting various cell-based applications, such as stem cell therapy, tissue engineering, and assisted reproduction. Recent times have witnessed an increase in the clinical demand of these applications, requiring urgent improvements in cell cryopreservation. However, cryopreservation technology suffers from the issues of low cryopreservation efficiency and cryoprotectant (CPA) toxicity. Application of advanced biotechnology tools can significantly improve post-thaw cell survival and reduce or even eliminate the use of organic solvent CPAs, thus promoting the development of cryopreservation. Herein, based on the different cryopreservation mechanisms available, we provide an overview of the applications and achievements of various biotechnology tools used in cell cryopreservation, including trehalose delivery, hydrogel-based cell encapsulation technique, droplet-based cell printing, and nanowarming, and also discuss the associated challenges and perspectives for future development.

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Current Status of Stem Cell Therapy and Nanofibrous Scaffolds in Cardiovascular Tissue Engineering

Regenerative Engineering and Translational Medicine ◽

10.1007/s40883-021-00230-1 ◽

2021 ◽

Author(s):

Aliakbar Yousefi-Ahmadipour ◽

Fatemeh Asadi ◽

Ali Pirsadeghi ◽

Niloofar Nazeri ◽

Reza Vahidi ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cell ◽

Cell Therapy ◽

Stem Cell Therapy ◽

Current Status ◽

Cardiovascular Tissue ◽

Nanofibrous Scaffolds ◽

Cardiovascular Tissue Engineering

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Evolving Concepts for Use of Stem Cells and Tissue Engineering for Cardiac Regeneration

Advances in Medical Technologies and Clinical Practice - Optimizing Assistive Technologies for Aging Populations ◽

10.4018/978-1-4666-9530-6.ch011 ◽

2016 ◽

pp. 279-313

Author(s):

Jahnavi Sarvepalli ◽

Rajalakshmi Santhakumar ◽

Rama Shanker Verma

Keyword(s):

Tissue Engineering ◽

Stem Cell ◽

Cell Therapy ◽

Stem Cell Therapy ◽

Treatment Options ◽

Treatment Strategies ◽

Basic Research ◽

Underlying Mechanism ◽

Repair Mechanisms ◽

New Treatment

The incidence of cardiovascular disease (CVD) in adults are increasing worldwide with impaired repair mechanisms, leading to tissue and organ failure. With the current advancements, life expectancy has improved and has led to search for new treatment strategies that improves tissue regeneration. Recently, stem cell therapy and tissue engineering has captured the attention of clinicians, scientists, and patients as alternative treatment options. The overall clinical experience of these suggests that they can be safely used in the right clinical setting. Ultimately, large outcome trials will have to be conducted to assess their efficacy. Clinical trials have to be carefully designed and patient safety must remain the key concern. At the same time, continued basic research is required to understand the underlying mechanism of cell-based therapies and cell tissue interactions. This chapter reviews the evolving paradigm of stem cell therapy and tissue engineering approaches for clinical application and explores its implications.

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