scholarly journals Comparison between Phage-ELISA and Phage Dot-Blot Assay Methods for the Detection of Hepatitis B Surface Antigen and its Antibodies in Human Serum

2014 ◽  
Vol 3 (4) ◽  
pp. 128-135
Author(s):  
Geok Tan ◽  
◽  
Wen Tan
Keyword(s):  
Hepatitis B ◽  
Human Serum ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Dot Blot ◽  
Phage Elisa ◽  
Dot Blot Assay ◽  
Assay Methods

scholarly journals Relationship of large hepatitis B surface antigen polypeptide to human serum albumin

1980 ◽  
Vol 28 (2) ◽  
pp. 459-463
Author(s):  
J W Shih ◽  
P L Tan ◽  
J L Gerin
Keyword(s):  
Molecular Weight ◽  
Human Serum Albumin ◽  
Hepatitis B ◽  
Serum Albumin ◽  
Human Serum ◽  
Surface Antigen ◽  
Plasma Proteins ◽  
Hepatitis B Surface Antigen ◽  
Significant Proportion ◽  
Relationship Of

A significant proportion (20 to 40%) of highly purified 22-nm hepatitis B surface antigen (HBsAg) particles contain human serum albumin (HSA) as demonstrated by specific precipitation of radioiodinated particles by anti-HSA. Preparations of the isolated major HBsAg polypeptides (P-1, P-2, and P-6) were iodinated and analyzed by radiommunoprecipitation for reactivity with rabbit antisera to human plasma proteins. Only the P-6 fraction (molecular weight, 68,000) was precipitated and only by anti-HSA; specific precipitation was observed with guinea pig antisera to P-6 and native HBsAg and goat or rabbit antisera to HSA. Coprecipitation of P-6 with antiserum to HBsAg and with anti-HSA, compared to precipitation with each antiserum alone, indicated that the HBsAg and HSA determinants were on separate molecules. The P-6 polypeptide may represent a precursor protein of the hepatitis B virion.

scholarly journals Antigenic Relationship of a Hepatitis B Surface Antigen-derived Polypeptide and Human Serum Albumin

1980 ◽  
Vol 48 (2) ◽  
pp. 273-283 ◽  
Author(s):  
Y. Sanchez ◽  
I. Ionescu-Matiu ◽  
F. B. Hollinger ◽  
J. L. Melnick ◽  
G. R. Dreesman
Keyword(s):  
Human Serum Albumin ◽  
Hepatitis B ◽  
Serum Albumin ◽  
Human Serum ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Antigenic Relationship ◽  
Relationship Of

Hepatitis B surface antigen binds to human serum albumin cross-linked by transglutaminase

Hepatology ◽  
1989 ◽  
Vol 9 (5) ◽  
pp. 726-730 ◽  
Author(s):  
Swan N. Thung ◽  
De-Fa Wang ◽  
Thomas M. Fasy ◽  
Andrew Hood ◽  
Michael A. Gerber
Keyword(s):  
Human Serum Albumin ◽  
Hepatitis B ◽  
Serum Albumin ◽  
Human Serum ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen
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