scholarly journals Role of the pelX gene of Klebsiella oxytoca VN13 in pectinolysis

2005 ◽  
Vol 21 (1) ◽  
pp. 55-59
Author(s):  
O. V. Lar ◽  
G. L. Kovtunovych ◽  
N. O. Kozyrovska
Keyword(s):  
Klebsiella Oxytoca

scholarly journals Bacterial nitrate assimilation: gene distribution and regulation

2011 ◽  
Vol 39 (6) ◽  
pp. 1838-1843 ◽  
Author(s):  
Víctor M. Luque-Almagro ◽  
Andrew J. Gates ◽  
Conrado Moreno-Vivián ◽  
Stuart J. Ferguson ◽  
David J. Richardson ◽  
...  
Keyword(s):  
Rhodobacter Capsulatus ◽  
Heterotrophic Bacteria ◽  
Azotobacter Vinelandii ◽  
Klebsiella Oxytoca ◽  
Prokaryotic Genome ◽  
Nitrate Assimilation ◽  
Primary Role ◽  
Bacterial Strains ◽  
Global Nitrogen Cycle

In the context of the global nitrogen cycle, the importance of inorganic nitrate for the nutrition and growth of marine and freshwater autotrophic phytoplankton has long been recognized. In contrast, the utilization of nitrate by heterotrophic bacteria has historically received less attention because the primary role of these organisms has classically been considered to be the decomposition and mineralization of dissolved and particulate organic nitrogen. In the pre-genome sequence era, it was known that some, but not all, heterotrophic bacteria were capable of growth on nitrate as a sole nitrogen source. However, examination of currently available prokaryotic genome sequences suggests that assimilatory nitrate reductase (Nas) systems are widespread phylogenetically in bacterial and archaeal heterotrophs. Until now, regulation of nitrate assimilation has been mainly studied in cyanobacteria. In contrast, in heterotrophic bacterial strains, the study of nitrate assimilation regulation has been limited to Rhodobacter capsulatus, Klebsiella oxytoca, Azotobacter vinelandii and Bacillus subtilis. In Gram-negative bacteria, the nas genes are subjected to dual control: ammonia repression by the general nitrogen regulatory (Ntr) system and specific nitrate or nitrite induction. The Ntr system is widely distributed in bacteria, whereas the nitrate/nitrite-specific control is variable depending on the organism.

scholarly journals Disulfide Bond Formation in Secreton Component PulK Provides a Possible Explanation for the Role of DsbA in Pullulanase Secretion

2001 ◽  
Vol 183 (4) ◽  
pp. 1312-1319 ◽  
Author(s):  
Anthony P. Pugsley ◽  
Nicolas Bayan ◽  
Nathalie Sauvonnet
Keyword(s):  
Disulfide Bond ◽  
Klebsiella Oxytoca ◽  
Bond Formation ◽  
Type Ii Secretion ◽  
Disulfide Bond Formation ◽  
Gene Encoding ◽  
Intramolecular Disulfide Bond ◽  
Disulfide Oxidoreductase ◽  
Considerable Excess

ABSTRACT When expressed in Escherichia coli, the 15Klebsiella oxytoca pul genes that encode the so-called Pul secreton or type II secretion machinery promote pullulanase secretion and the assembly of one of the secreton components, PulG, into pili. Besides these pul genes, efficient pullulanase secretion also requires the host dsbA gene, encoding a periplasmic disulfide oxidoreductase, independently of disulfide bond formation in pullulanase itself. Two secreton components, the secretin pilot protein PulS and the minor pseudopilin PulK, were each shown to posses an intramolecular disulfide bond whose formation was catalyzed by DsbA. PulS was apparently destabilized by the absence of its disulfide bond, whereas PulK stability was not dramatically affected either by adsbA mutation or by the removal of one of its cysteines. The pullulanase secretion defect in a dsbA mutant was rectified by overproduction of PulK, indicating reduced disulfide bond formation in PulK as the major cause of the secretion defect under the conditions tested (in which PulS is probably present in considerable excess of requirements). PulG pilus formation was independent of DsbA, probably because PulK is not needed for piliation.

scholarly journals Role of the exopolygalacturonase gene in interaction of Klebsiella oxytoca VN13 with wheat roots

2002 ◽  
Vol 18 (4) ◽  
pp. 319-323 ◽  
Author(s):  
G. L. Kovtunovych ◽  
O. V. Lar ◽  
N. O. Kozyrovska
Keyword(s):  
Klebsiella Oxytoca ◽  
Wheat Roots

Role of Klebsiella Oxytoca in non-hemorrhagic antibiotic-associated diarrhea

2008 ◽  
Vol 46 (06) ◽  
Author(s):  
I Zollner-Schwetz ◽  
C Högenauer ◽  
M Joainig ◽  
P Weberhofer ◽  
G Gorkiewicz ◽  
...  
Keyword(s):  
Klebsiella Oxytoca ◽  
Antibiotic Associated Diarrhea

The Role of Sorption in the Transport of Klebsiella oxytoca Through Saturated Silica Sand

Ground Water ◽  
1997 ◽  
Vol 35 (4) ◽  
pp. 574-584 ◽  
Author(s):  
M. J. Hendry ◽  
J. R. Lawrence ◽  
P. Maloszewski
Keyword(s):  
Klebsiella Oxytoca ◽  
Silica Sand

scholarly journals The Contribution of Polysaccharide Antigens From Clinical Proteus spp. and Klebsiella spp. Isolates to the Serological Cross-Reactions

2021 ◽  
Vol 11 ◽  
Author(s):  
Agata Palusiak
Keyword(s):  
Western Blotting ◽  
Silver Staining ◽  
Klebsiella Oxytoca ◽  
Immunological Response ◽  
Staining Procedure ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cross Reactions ◽  
Polysaccharide Antigens ◽  
Tract Infections

Klebsiella spp. and Proteus spp. cause hospital-acquired urinary tract infections (UTIs), which are often related to the use of catheters. To create a vaccine preventing UTI, immunogenic bacterial antigens with common epitopes are still being looked for. In this work, the role of polysaccharide antigens of four Klebsiella spp. and eight Proteus spp. strains in serological cross-reactions with specific antisera was examined. Enzyme-linked immunosorbent assay (ELISA), Western blotting, and silver staining by Tsai method were performed. The Klebsiella and Proteus spp. LPSs and cells were used as antigens. Polyclonal rabbit sera specific to Klebsiella oxytoca 0.023 and 0.062 strains and four Klebsiella spp. LPSs were obtained. The ELISA and Western blotting results showed the strongest cross-reactions occurring between lipopolysaccharides (LPSs) from four Klebsiella strains and P. vulgaris O42 antiserum. The silver-staining procedure revealed the patterns typical of both slow- and fast-migrating mass species of the Klebsiella LPSs. The Klebsiella spp. antigens also cross-reacted with four P. penneri antisera, and most of the reactions were observed as low-migrating patterns. From two K. oxytoca antisera obtained in this work, only one, the K. oxytoca 0.062 antiserum, cross-reacted with satisfactory strength with P. penneri LPSs (19, 22, and 60). Obtaining cross-reactions between the antigens of Klebsiella strains and Proteus antisera and in the opposite systems is important for proving the immunogenic role of polysaccharide antigens in triggering the immunological response.

scholarly journals The role of biofertilizers in improving vegetative growth, yield and fruit quality of apple

2018 ◽  
Vol 45 (No. 4) ◽  
pp. 173-180
Author(s):  
Walid Fediala Abd El-Gleel Mosa ◽  
Lidia Sas Paszt ◽  
Mateusz Frąc ◽  
Paweł Trzciński ◽  
Waldemar Treder ◽  
...  
Keyword(s):  
Fruit Quality ◽  
Klebsiella Oxytoca ◽  
Growth Yield ◽  
Quality Parameters ◽  
Bacterial Strains ◽  
Apple Trees ◽  
Rhizobium Sp ◽  
Npk Fertilization

Apple trees which grafted on M.M. 106 stocks were planted at the beginning of spring 2014 in pots at the Research Institute of Horticulture in Skierniewice, Poland. The used treatments in the experiment were: NPK fertilization and bioproducts: Fertigo (Manure), Micosat, Humus UP, Humus Active + Aktywit PM, BioFeed Quality, BioFeed Amin, Vinassa, Florovit Natura and Florovit Eko. In the spring, these treatments were applied to apple trees alone or enriched with Pantoea sp., Pseudomonas fluorescens, Klebsiella oxytoca and Rhizobium sp. bacterial strains. Growth, yield and fruit quality parameters were evaluated. The results revealed that photosynthetic rate was greatly improved by the addition of bacteria to Fertigo (Manure), Micosat, Humus UP and Humus Active + Aktywit PM as compared to NPK. Tree trunk thickness was significantly enhanced with Vinassa, Florovit Natura and Florovit Eko enriched with bacteria. The application of bacterial strains increased the effectiveness of Fertigo (Manure), Humus UP, Humus Active + Aktywit PM, Biofeed Amin and Yeast in increasing significantly number and weight of fruits as compared to NPK. 

scholarly journals Role of TolC in Klebsiella oxytoca resistance to antibiotics

2009 ◽  
Vol 63 (4) ◽  
pp. 668-674 ◽  
Author(s):  
A. Fenosa ◽  
E. Fuste ◽  
L. Ruiz ◽  
P. Veiga-Crespo ◽  
T. Vinuesa ◽  
...  
Keyword(s):  
Klebsiella Oxytoca ◽  
Resistance To Antibiotics

scholarly journals Membrane Association and Multimerization of Secreton Component PulC

1999 ◽  
Vol 181 (13) ◽  
pp. 4004-4011 ◽  
Author(s):  
Odile M. Possot ◽  
Manon Gérard-Vincent ◽  
Anthony P. Pugsley
Keyword(s):  
Outer Membrane ◽  
Klebsiella Oxytoca ◽  
Subcellular Fractionation ◽  
Whole Cells ◽  
Outer Membranes ◽  
Specific Serum ◽  
Signal Anchor ◽  
Anchor Sequence ◽  
Exoprotein Secretion

ABSTRACT The PulC component of the Klebsiella oxytocapullulanase secretion machinery (the secreton) was found by subcellular fractionation to be associated with both the cytoplasmic (inner) and outer membranes. Association with the outer membrane was independent of other secreton components, including the outer membrane protein PulD (secretin). The association of PulC with the inner membrane is mediated by the signal anchor sequence located close to its N terminus. These results suggest that PulC forms a bridge between the two membranes that is disrupted when bacteria are broken open for fractionation. Neither the signal anchor sequence nor the cytoplasmic N-terminal region that precedes it was found to be required for PulC function, indicating that PulC does not undergo sequence-specific interactions with other cytoplasmic membrane proteins. Cross-linking of whole cells resulted in the formation of a ca. 110-kDa band that reacted with PulC-specific serum and whose detection depended on the presence of PulD. However, antibodies against PulD failed to react with this band, suggesting that it could be a homo-PulC trimer whose formation requires PulD. The data are discussed in terms of the possible role of PulC in energy transduction for exoprotein secretion.

Role of dactinomycin in the improved survival of children with Wilms' tumor

JAMA ◽  
1966 ◽  
Vol 195 (12) ◽  
pp. 1005-1009 ◽  
Author(s):  
D. J. Fernbach
Keyword(s):  
Wilms Tumor
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