Relative telomere length of the residents of Lviv oblast

Aim. Telomeres are short, tandem repeats of DNA that cap linear chromosome ends by binding members of the shelterin protein complex to form protective telomere loops. An insufficient number of telomere repeats leads to chromosome uncapping, cell senescence, and death. Aim of this thesis is the analysis of relative telomere length (RTL) in whole blood among residents of Lviv region. Methods. The RTL in the whole blood cells was measured in 86 residents of various age (47 men and 39 women aged 18–72) using quantitative real-time PCR (Cawthon’s method). It is based on the simultaneous amplification of telomeric repeats (T) and a single copy gene (S). Results. There is a tendency to decrease RTL with age. The low correlation between RTL and age can be linked to various factors, including the heterogeneity of telomere length at birth, chronic socioeconomic stress, genetic determinism, sensitivity to exogenous pressures. Women and men did not differ significantly in the rate of RTL shortening. Conclusions. The results indicate the absence of reliable differences relative telomere length in individuals of different sexes. The low correlation between RTL and age can be linked to various factors, including insufficient sample size of people aged over 50 years. So in the future we plan to continue these studies in older people. Keywords: relative telomere length, RT-PCR, age, gender.

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Telomere Instability in Lynch Syndrome Families Leads to Some Shorter Telomeres in MSH2+/- Carriers

Life ◽

10.3390/life10110265 ◽

2020 ◽

Vol 10 (11) ◽

pp. 265

Author(s):

M. Carmen Garrido-Navas ◽

Frances Tippins ◽

Julian Barwell ◽

Jonathan Hoffman ◽

Veryan Codd ◽

...

Keyword(s):

Lynch Syndrome ◽

Telomere Length ◽

Short Tandem Repeats ◽

Tandem Repeats ◽

Cell Turnover ◽

Wild Type ◽

Telomeric Dna ◽

Dna Mismatch ◽

And Control ◽

Short Tandem

Lynch syndrome (LS) is an inherited predisposition to early onset of various cancers, caused by mutation in a DNA mismatch repair (MMR) gene. In heterozygous MMR+/− carriers, somatic mutation, loss or silencing of the wild type allele increases the mutation rate, facilitating the initiation of MMR-defective cancers. These cancers are characterized by instability at short tandem repeats (STRs) and in telomeric DNA. We have investigated telomere length in saliva DNA from LS and control families, using single telomere analysis at XpYp and 12q and by qPCR to measure total telomeric DNA. Single telomere analysis showed a trend for shorter XpYp telomeres in MSH2+/− carriers compared to MLH1+/− carriers or controls, but this was masked in the comparative analysis of total telomeric DNA. Comparison of age-adjusted telomere length within families showed that neither MSH2+/− or MLH1+/− children had consistently shorter or longer telomeres than their MMR+/− parent, indicating the absence of an inter-generational effect on telomere length. Unexpectedly however, wildtype children in families with MSH2 mutations, had significantly longer XpYp telomeres than their MMR+/− parent. Altogether our data suggest that MMR insufficiency, particularly in MSH2+/− carriers, increases telomere instability and somatic cell turnover during the lifetime of LS mutation carriers but has minimal consequences for telomere length in the germline.

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Faculty Opinions recommendation of Abundant contribution of short tandem repeats to gene expression variation in humans.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.725994101.793513449 ◽

2016 ◽

Author(s):

Melanie Bahlo

Keyword(s):

Gene Expression ◽

Short Tandem Repeats ◽

Tandem Repeats ◽

Gene Expression Variation ◽

Expression Variation ◽

Short Tandem

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Faculty Opinions recommendation of Identification of embryo-fetal cells in celomic fluid using morphological and short-tandem repeats analysis.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.726727769.793540302 ◽

2017 ◽

Author(s):

Eliezer Rachmilewitz ◽

Eitan Fibach

Keyword(s):

Short Tandem Repeats ◽

Tandem Repeats ◽

Fetal Cells ◽

Short Tandem

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Population Genetics of Y-Chromosome Short Tandem Repeats in Humans

Journal of Molecular Evolution ◽

10.1007/pl00006229 ◽

1997 ◽

Vol 45 (3) ◽

pp. 265-270 ◽

Cited By ~ 49

Author(s):

Anna Pérez-Lezaun ◽

Francesc Calafell ◽

Mark Seielstad ◽

Eva Mateu ◽

David Comas ◽

...

Keyword(s):

Population Genetics ◽

Y Chromosome ◽

Short Tandem Repeats ◽

Tandem Repeats ◽

Short Tandem

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Conditional Genotypic Probabilities for Microsatellite Loci

Genetics ◽

10.1093/genetics/155.4.1973 ◽

2000 ◽

Vol 155 (4) ◽

pp. 1973-1980

Author(s):

Jinko Graham ◽

James Curran ◽

B S Weir

Keyword(s):

Short Tandem Repeats ◽

Tandem Repeats ◽

Dirichlet Distribution ◽

Forensic Dna ◽

Match Probability ◽

Microsatellite Genotype ◽

Allelic State ◽

Forensic Assessments ◽

Dna Profiles ◽

Short Tandem

Abstract Modern forensic DNA profiles are constructed using microsatellites, short tandem repeats of 2–5 bases. In the absence of genetic data on a crime-specific subpopulation, one tool for evaluating profile evidence is the match probability. The match probability is the conditional probability that a random person would have the profile of interest given that the suspect has it and that these people are different members of the same subpopulation. One issue in evaluating the match probability is population differentiation, which can induce coancestry among subpopulation members. Forensic assessments that ignore coancestry typically overstate the strength of evidence against the suspect. Theory has been developed to account for coancestry; assumptions include a steady-state population and a mutation model in which the allelic state after a mutation event is independent of the prior state. Under these assumptions, the joint allelic probabilities within a subpopulation may be approximated by the moments of a Dirichlet distribution. We investigate the adequacy of this approximation for profiled loci that mutate according to a generalized stepwise model. Simulations suggest that the Dirichlet theory can still overstate the evidence against a suspect with a common microsatellite genotype. However, Dirichlet-based estimators were less biased than the product-rule estimator, which ignores coancestry.

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