scholarly journals Genetic polymorphism of white mistletoe (Viscum album L.) in Ukraine

2021 ◽  
Vol 28 ◽  
pp. 36-41
Author(s):  
Yu. O. Bilonozhko ◽  
A. M. Rabokon ◽  
A. S. Postovoitova ◽  
L. O. Kalafat ◽  
N. S. Boiko ◽  
...  
Keyword(s):  
Length Polymorphism ◽  
Polyacrylamide Gel Electrophoresis ◽  
Viscum Album ◽  
Intron Length ◽  
Intron Length Polymorphism ◽  
Tubulin Genes ◽  
Silver Nitrate Staining ◽  
Dna Profiles ◽  
Different Parts ◽  
Β Tubulin

Aim. The aim of the study was to establish genetic differences between V. album growing in different parts of Ukraine. Methods. White mistletoe samples collected in different regions of Ukraine were used in the study. The method of estimating the intron length polymorphism of β-tubulin genes was used. Amplified DNA fragments were fractionated by non-denaturing polyacrylamide gel electrophoresis and visualized by silver nitrate staining. Results. The genotypes of 91 white mistletoe plants were analyzed. DNA profiles of white mistletoe with a specific amplicons of β-tubulin gene introns were obtained, which allowed to differentiate the samples from each other. Fingerprinting data were used for cluster analysis and dendrogram construction. Conclusions. It was found that the analyzed mistletoe samples did not differ by geographical factor and were characterized by a low level of genetic diversity in the studied samples. Keywords: Viscum album L., intron length polymorphism, β-tubulin, genetic variability, Ukraine.

scholarly journals Intravarietal intron-length polymorphism of β-tubulin genes in belorussian landraces of Linum usitatissimum L.

2018 ◽  
Vol 22 ◽  
pp. 180-185
Author(s):  
A. M. Rabokon ◽  
Ya. B. Pirko ◽  
L. O. Kalafat ◽  
Ye. V. Guzenko ◽  
M. V. Bogdanova ◽  
...  
Keyword(s):  
Length Polymorphism ◽  
Linum Usitatissimum ◽  
Intron Length ◽  
Intron Length Polymorphism ◽  
Linum Usitatissimum L ◽  
Tubulin Genes ◽  
Molecular Phylogenetic ◽  
Silver Nitrate Staining ◽  
Molecular Selection ◽  
Β Tubulin

Aim. The aim of the work was to evaluate the possibilities of using the β-tubulin intron length (TBP, Tubulin Based Polymorphism) for genetic differentiation of ancient flax varieties (landraces), plants that was historically formed in Belarus. Methods. The β-tubulin first intron length polymorphism evaluating method (TBP) was used. Amplified fragments (β-tubulin introns) were fractionated by electrophoresis in non-denaturing polyacrylamide gel. DNA bands were detected using silver nitrate staining. Results. The size of the amplified fragments varied from 400 bp to 1900 bp. It was found that 25 of 30 studied varieties (landraces) were genetically heterogeneous. The total number of allele phenotypes was 7, and the value of PIC (Polymorphism Information Content) varied from 0.0 to 0.72. Conclusions. The data obtained make it possible to recommend landraces as a source of genes for increasing the genetic diversity of the existing flax gene pool, and the TBP method can be applied both in molecular-phylogenetic analysis and in molecular selection of flax. Keywords: Linum usitatissimum, landrace, molecular markers, DNA fingerprinting, β-tubulin genes, intron polymorphism.

Intron length polymorphism of β‐tubulin genes of Aegilops biuncialis Vis

2017 ◽  
Vol 43 (9) ◽  
pp. 1031-1039 ◽  
Author(s):  
Anastasiia Rabokon ◽  
Andrii Demkovych ◽  
Alexei Sozinov ◽  
Natalia Kozub ◽  
Igor Sozinov ◽  
...  
Keyword(s):  
Length Polymorphism ◽  
Intron Length ◽  
Intron Length Polymorphism ◽  
Tubulin Genes ◽  
Β Tubulin

scholarly journals Intron length polymorphism of β-tubulin genes in microalgae

2019 ◽  
Vol 1 ◽  
pp. 93-99
Author(s):  
Ya.V. Pirko ◽  
◽  
A.M. Rabokon ◽  
A.S. Postovoitova ◽  
Y.O. Bilonozhko ◽  
...  
Keyword(s):  
Length Polymorphism ◽  
Intron Length ◽  
Intron Length Polymorphism ◽  
Tubulin Genes ◽  
Β Tubulin

scholarly journals Estimation of genetic diversity for different species of woody plants by intron length polymorphism of beta-tubulin genes

2018 ◽  
Vol 15 (2) ◽  
pp. 159-166
Author(s):  
L. O. Kalafat ◽  
N. N. Pirko ◽  
A. Ye. Demkovych ◽  
S. N. Privalikhin ◽  
A. N. Rabokon ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Fagus Sylvatica ◽  
Length Polymorphism ◽  
Juglans Regia ◽  
Molecular Genetic ◽  
Robinia Pseudoacacia ◽  
Intron Length ◽  
Intron Length Polymorphism ◽  
Tubulin Genes ◽  
Β Tubulin

Aim. The effectiveness of using the method of intron length polymorphism of β-tubulin genes is analyzed to evaluate the genetic diversity and polymorphism of tree plants by approbating it for a broad sample of tree plants of different taxonomic positions. Method. The method of estimation intron length polymorphism of β- tubulin (tubulin base polymorphism — TBP-method) has been tested. Results. The molecular genetic profiles and the unique patterns for the Quercus robur L., Populus tremula L., Fagus sylvatica L., Fagus sylvatica f. salicifolia, Robinia pseudoacacia L., Morus alba L., Ulmus glabra Huds., Betula pendula Roth., Acer platanoides L., Acer negundo L., Acer saccharinum Marshall, Catalpa bignonioides Walter, Tilia cordata Mill., Tilia platyphyllos Scop., Aesculus hippocastanum L., Populus nigra L., Juglans regia L., Fraxinus excelsior L., Alnus glutinosa (L.) Gaertn., Ginkgo biloba L. have been created. Some common fragments inherent in individual genera within the family have been found. Conclusions. TBP-method is rather convenient and reliable. It can be used both for molecular genetic marking and for the study of intra- and interspecific polymorphism of economically valuable, horticultural and forest trees.Keywords: TBP-method, β-tubulin introns, tree plants, genetic diversity.

scholarly journals Distribution of mistletoe (Viscum album L.), which parasitizes different woody plants species, in Kyiv and its genetic characteristics

2019 ◽  
Vol 25 ◽  
pp. 106-110
Author(s):  
Yu. O. Bilonozhko ◽  
L. O. Ponomarenko ◽  
A. M. Rabokon ◽  
A. S. Postovoitovа ◽  
L. O. Kalafat ◽  
...  
Keyword(s):  
Species Diversity ◽  
Length Polymorphism ◽  
Molecular Genetic ◽  
Viscum Album ◽  
Intron Length Polymorphism ◽  
Genetic Characteristics ◽  
Host Trees ◽  
Silver Nitrate Staining ◽  
Genetic Profiles ◽  
Β Tubulin

Aim. The aim of the work was to study the species diversity of mistletoe host trees with the establishment of damage degrees and the study of the mistletoe genetic characteristics in Kiev. Methods. The β-tubulin first intron length polymorphism evaluating method (TBP) was used. Amplified fragments DNA were fractionated by electrophoresis in non-denaturing polyacrylamide gel. DNA bands were detected using silver nitrate staining. Results. The species diversity of Viscum album L. host-trees was studied in Kiev. Among them, plants of the genus Pinus and angiosperms that belong to 8 genera (Acer, Fraxinus, Acacia, Populus, Tilia, Salix, Malus, Sorbus), wherein 47% belong to the genus Acer. It was revealed that among infected trees, 30% had severe damage to mistletoe, 28% moderate and 42% weak. Based on the analysis of DNA marker polymorphisms, the molecular-genetic profiles of V. album, which grows on various species of host trees, were obtained and analyzed. Conclusions. The species diversity of V. album host-trees was studied and the degree of their infection was assessed in Kiev. Mistletoe, which grows on angiosperms, is characterized by a greater degree of genetic polymorphism than that which grows on gymnosperms. Keywords: Viscum album L., host-plant, introns of genes, length polymorphism, β-tubulin.

scholarly journals Study of intron length polymorphism of the α-tubulin genes as a method of analysis the genetic differentiation in plants

2019 ◽  
Vol 75 (6) ◽  
pp. 576-584 ◽  
Author(s):  
Ya.V. Pirko ◽  
◽  
A.S. Postovoitova ◽  
A.M. Rabokon ◽  
L.O. Kalafat ◽  
...  
Keyword(s):  
Genetic Differentiation ◽  
Length Polymorphism ◽  
Intron Length ◽  
Intron Length Polymorphism ◽  
Tubulin Genes
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