scholarly journals A Comprehensive Study of High Cholesterol Diet-Induced Larval Zebrafish Model: A Short-Time In Vivo Screening Method for Non-Alcoholic Fatty Liver Disease Drugs

2019 ◽  
Vol 15 (5) ◽  
pp. 973-983 ◽  
Author(s):  
Ji Ma ◽  
Hongli Yin ◽  
Maoru Li ◽  
Yang Deng ◽  
Owais Ahmad ◽  
...  
Keyword(s):  
Screening Method ◽  
High Cholesterol Diet ◽  
High Cholesterol ◽  
Zebrafish Model ◽  
Alcoholic Fatty Liver ◽  
Larval Zebrafish ◽  
Short Time ◽  
Alcoholic Fatty Liver Disease ◽  
Comprehensive Study

scholarly journals Kaempferol-3-O-Glucuronide Ameliorates Non-Alcoholic Steatohepatitis in High-Cholesterol-Diet-Induced Larval Zebrafish and HepG2 Cell Models via Regulating Oxidation Stress

Life ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 445
Author(s):  
Yang Deng ◽  
Ji Ma ◽  
Xin Weng ◽  
Yuqin Wang ◽  
Maoru Li ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Accumulation ◽  
Hepg2 Cells ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Zebrafish Model ◽  
Alcoholic Fatty Liver ◽  
Larval Zebrafish

NAFLD (non-alcoholic fatty liver disease) is one of the most prominent liver diseases in the world. As a metabolic-related disease, the development of NAFLD is closely associated with various degrees of lipid accumulation, oxidation, inflammation, and fibrosis. Ilex chinensis Sims is a form of traditional Chinese medicine which is used to treat bronchitis, burns, pneumonia, ulceration, and chilblains. Kaempferol-3-O-glucuronide (K3O) is a natural chemical present in Ilex chinensis Sims. This study was designed to investigate the antioxidative, fat metabolism-regulating, and anti-inflammatory potential of K3O. A high-cholesterol diet (HCD) was used to establish steatosis in larval zebrafish, whereby 1mM free fatty acid (FFA) was used to induce lipid accumulation in HepG2 cells, while H2O2 was used to induce oxidative stress in HepG2. The results of this experiment showed that K3O reduced lipid accumulation and the level of reactive oxygen species (ROS) both in vivo (K3O, 40μM) and in vitro (K3O, 20μM). Additionally, K3O (40μM) reduced neutrophil aggregation in vivo. K3O (20μM) also decreased the level of malondialdehyde (MDA) and significantly increased the level of glutathione peroxidase (GSH-px) in both the HCD-induced larval zebrafish model and H2O2-exposed HepG2 cells. In the mechanism study, keap1, nrf2, tnf-α, and il-6 mRNA were all significantly reversed by K3O (20μM) in zebrafish. Changes in Keap1 and Nrf2 mRNA expression were also detected in H2O2-exposed HepG2 cells after they were treated with K3O (20μM). In conclusion, K3O exhibited a reduction in oxidative stress and lipid peroxidation, and this may be related to the Nrf2/Keap1 pathway in the NAFLD larval zebrafish model.

Bifidobacterium adolescentis and Lactobacillus rhamnosus alleviate non-alcoholic fatty liver disease induced by a high-fat, high-cholesterol diet through modulation of different gut microbiota-dependent pathways

2020 ◽  
Vol 11 (7) ◽  
pp. 6115-6127
Author(s):  
Gang Wang ◽  
Ting Jiao ◽  
Yue Xu ◽  
Daozheng Li ◽  
Qian Si ◽  
...  
Keyword(s):  
Liver Disease ◽  
Gut Microbiota ◽  
Fatty Liver ◽  
Fatty Liver Disease ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
High Fat ◽  
Alcoholic Fatty Liver ◽  
Alcoholic Fatty Liver Disease

B. adolescentis and L. rhamnosus alleviate non-alcoholic fatty liver disease induced by a high-fat, high-cholesterol diet through modulation on different gut microbiota-dependent pathways. The SCFAs are important participants.

scholarly journals A Larval Zebrafish Model for Assessing Hypoxic‐Induced In Vivo Cardiomyocyte Damage: Time Course for Induction and Cardiac Output Recovery

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Warren Burggren ◽  
Regina Abramova ◽  
Naim Bautista ◽  
Regina Fritsche Danielson ◽  
Avi Gupta ◽  
...  
Keyword(s):  
Cardiac Output ◽  
Time Course ◽  
Zebrafish Model ◽  
Larval Zebrafish

scholarly journals In VivoHypocholesterolemic Effect of MARDI Fermented Red Yeast Rice Water Extract in High Cholesterol Diet Fed Mice

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Swee Keong Yeap ◽  
Boon Kee Beh ◽  
Joan Kong ◽  
Wan Yong Ho ◽  
Hamidah Mohd Yusof ◽  
...  
Keyword(s):  
Water Extract ◽  
Lipid Lowering ◽  
High Cholesterol Diet ◽  
Red Yeast Rice ◽  
Monacolin K ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Red Yeast ◽  
Rice Water

Fermented red yeast rice has been traditionally consumed as medication in Asian cuisine. This study aimed to determine thein vivohypocholesterolemic and antioxidant effects of fermented red yeast rice water extract produced using Malaysian Agricultural Research and Development Institute (MARDI)Monascus purpureusstrains in mice fed with high cholesterol diet. Absence of monacolin-k, lower level ofγ-aminobutyric acid (GABA), higher content of total amino acids, and antioxidant activities were detected in MARDI fermented red yeast rice water extract (MFRYR).In vivoMFRYR treatment on hypercholesterolemic mice recorded similar lipid lowering effect as commercial red yeast rice extract (CRYR) as it helps to reduce the elevated serum liver enzyme and increased the antioxidant levels in liver. This effect was also associated with the upregulation of apolipoproteins-E and inhibition of Von Willebrand factor expression. In summary, MFRYR enriched in antioxidant and amino acid without monacolin-k showed similar hypocholesterolemic effect as CRYR that was rich in monacolin-k and GABA.

scholarly journals Complications of Non-Alcoholic Fatty Liver Disease in Extrahepatic Organs

Diagnostics ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 912
Author(s):  
Wataru Tomeno ◽  
Kento Imajo ◽  
Takuya Takayanagi ◽  
Yu Ebisawa ◽  
Kosuke Seita ◽  
...  
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Cause Of Death ◽  
Fatty Liver Disease ◽  
Sleep Apnea Syndrome ◽  
Screening Method ◽  
Alcoholic Fatty Liver ◽  
Obstructive Sleep ◽  
Alcoholic Fatty Liver Disease ◽  
Related Mortality

Non-alcoholic fatty liver disease (NAFLD) is now recognized as the most common chronic liver disease worldwide, along with the concurrent epidemics of metabolic syndrome and obesity. Patients with NAFLD have increased risks of end-stage liver disease, hepatocellular carcinoma, and liver-related mortality. However, the largest cause of death among patients with NAFLD is cardiovascular disease followed by extrahepatic malignancies, whereas liver-related mortality is only the third cause of death. Extrahepatic complications of NAFLD include chronic kidney disease, extrahepatic malignancies (such as colorectal cancer), psychological dysfunction, gastroesophageal reflux disease, obstructive sleep apnea syndrome, periodontitis, hypothyroidism, growth hormone deficiency, and polycystic ovarian syndrome. The objective of this narrative review was to summarize recent evidences about extrahepatic complications of NAFLD, with focus on the prevalent/incident risk of such diseases in patients with NAFLD. To date, an appropriate screening method for extrahepatic complications has not yet been determined. Collaborative care with respective experts seems to be necessary for patient management because extrahepatic complications can occur across multiple organs. Further studies are needed to reveal risk profiles at baseline and to determine an appropriate screening method for extrahepatic diseases.

P0975 : Characterization of an in vivo model of juvenile non-alcoholic fatty liver disease

2015 ◽  
Vol 62 ◽  
pp. S711-S712
Author(s):  
V. Marin ◽  
N. Rosso ◽  
M. Dal Ben ◽  
A. Raseni ◽  
C. Degrassi ◽  
...  
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Fatty Liver Disease ◽  
In Vivo Model ◽  
Alcoholic Fatty Liver ◽  
Alcoholic Fatty Liver Disease
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