scholarly journals Peer Review #2 of "Can non-destructive DNA extraction of bulk invertebrate samples be used for metabarcoding? (v0.1)"

2018 ◽  
Author(s):  
T Ekrem
Keyword(s):  
Peer Review ◽  
Dna Extraction ◽  
Non Destructive

scholarly journals Non-destructive genetic sampling in fish. An improved method for DNA extraction from fish fins and scales

Hereditas ◽  
2003 ◽  
Vol 138 (3) ◽  
pp. 161-165 ◽  
Author(s):  
ADRIANE P. WASKO ◽  
CESAR MARTINS ◽  
CLAUDIO OLIVEIRA ◽  
FAUSTO FORESTI
Keyword(s):  
Dna Extraction ◽  
Improved Method ◽  
Genetic Sampling ◽  
Fish Fins ◽  
Non Destructive

Tu1709 An Efficient and Non-Destructive Method for Microbial DNA Extraction From Paraffin-Embedded Intestinal Biopsies

2013 ◽  
Vol 144 (5) ◽  
pp. S-827
Author(s):  
Franck Carbonero ◽  
Ann C. Benefiel ◽  
Jona Kristo ◽  
Jenna K. Leinberger ◽  
Eugene Greenberg ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Microbial Dna ◽  
Non Destructive

Non-destructive DNA extraction method for identification of Bradysia odoriphaga (Diptera: Sciaridae), a pest of Welsh onion, carrot, and Chinese chive in Japan

2019 ◽  
Vol 55 (1) ◽  
pp. 181-185
Author(s):  
Makoto Arimoto ◽  
Norihide Hinomoto ◽  
Ryosuke Omata ◽  
Ryozaburo Iwase ◽  
Masahiro Sueyoshi ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Extraction Method ◽  
Welsh Onion ◽  
Dna Extraction Method ◽  
Chinese Chive ◽  
Non Destructive

scholarly journals A new, harmless, high-throughput endosperm-based DNA extraction method for wheat

2019 ◽  
Vol 49 (9) ◽  
Author(s):  
Zhihui Ma ◽  
Yuquan Wang ◽  
Wenhui Wei ◽  
Zhengang Ru
Keyword(s):  
Dna Extraction ◽  
High Throughput ◽  
Extraction Method ◽  
Seedling Emergence ◽  
Control Group ◽  
Suitable Alternative ◽  
Dna Extraction Method ◽  
High Throughput Method ◽  
Young Leaves ◽  
Non Destructive

ABSTRACT: In this study, a non-destructive, high-throughput, endosperm-based DNA extraction method was developed. To verify the non-destructive nature of this method, a germination test was performed on 288 seeds after sampling their endosperm, which gave a seedling emergence rate that was higher (97.6%) than that of the control group (92%). To confirm the feasibility of the new method, DNA was extracted from plants of a BC1F2 population by two different methods, namely, from endosperm using our rapid, high-throughput method (ER-DNA) and from young leaves emerging from the same sampled seed using the CTAB method (LC-DNA). The ER-DNA was undetectable by agarose gel electrophoresis, but was found to be an adequate replacement for LC-DNA for the amplification and detection of simple sequence repeats (SSRs). Further analysis revealed that ER-DNA was generally suitable for the generation of specific 500-750-bp fragments, but not for the amplification of 1,000-2,000-bp fragments. Our rapid, high-throughput method therefore has no deleterious effects on wheat seeds and yields DNA for SSR genotyping that is a suitable alternative to traditionally obtained DNA.

scholarly journals Can non-destructive DNA extraction of bulk invertebrate samples be used for metabarcoding?

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4980 ◽  
Author(s):  
Melissa E. Carew ◽  
Rhys A. Coleman ◽  
Ary A. Hoffmann
Keyword(s):  
Dna Extraction ◽  
Dna Barcode ◽  
Extraction Methods ◽  
Biological Communities ◽  
Freshwater Invertebrate ◽  
High Throughput Dna Sequencing ◽  
Dna Extraction Methods ◽  
Taxonomic Groups ◽  
Taxonomic Studies ◽  
Non Destructive

Background High throughput DNA sequencing of bulk invertebrate samples or metabarcoding is becoming increasingly used to provide profiles of biological communities for environmental monitoring. As metabarcoding becomes more widely applied, new reference DNA barcodes linked to individual specimens identified by taxonomists are needed. This can be achieved through using DNA extraction methods that are not only suitable for metabarcoding but also for building reference DNA barcode libraries. Methods In this study, we test the suitability of a rapid non-destructive DNA extraction method for metabarcoding of freshwater invertebrate samples. Results This method resulted in detection of taxa from many taxonomic groups, comparable to results obtained with two other tissue-based extraction methods. Most taxa could also be successfully used for subsequent individual-based DNA barcoding and taxonomic identification. The method was successfully applied to field-collected invertebrate samples stored for taxonomic studies in 70% ethanol at room temperature, a commonly used storage method for freshwater samples. Discussion With further refinement and testing, non-destructive extraction has the potential to rapidly characterise species biodiversity in invertebrate samples, while preserving specimens for taxonomic investigation.

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