Non-destructive DNA extraction method for identification of Bradysia odoriphaga (Diptera: Sciaridae), a pest of Welsh onion, carrot, and Chinese chive in Japan

ABSTRACT: In this study, a non-destructive, high-throughput, endosperm-based DNA extraction method was developed. To verify the non-destructive nature of this method, a germination test was performed on 288 seeds after sampling their endosperm, which gave a seedling emergence rate that was higher (97.6%) than that of the control group (92%). To confirm the feasibility of the new method, DNA was extracted from plants of a BC1F2 population by two different methods, namely, from endosperm using our rapid, high-throughput method (ER-DNA) and from young leaves emerging from the same sampled seed using the CTAB method (LC-DNA). The ER-DNA was undetectable by agarose gel electrophoresis, but was found to be an adequate replacement for LC-DNA for the amplification and detection of simple sequence repeats (SSRs). Further analysis revealed that ER-DNA was generally suitable for the generation of specific 500-750-bp fragments, but not for the amplification of 1,000-2,000-bp fragments. Our rapid, high-throughput method therefore has no deleterious effects on wheat seeds and yields DNA for SSR genotyping that is a suitable alternative to traditionally obtained DNA.

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A simple, non-destructive frozen DNA extraction method for viruliferous whiteflies collected from a yellow sticky trap plate to detect tomato yellow leaf curl virus

Annual Report of The Kansai Plant Protection Society ◽

10.4165/kapps.56.127 ◽

2014 ◽

Vol 56 (0) ◽

pp. 127-129

Author(s):

Shuko Toyoda ◽

Toshio Kitamura ◽

Mitsuyoshi Takeda ◽

Jun Ohnishi

Keyword(s):

Dna Extraction ◽

Extraction Method ◽

Tomato Yellow Leaf ◽

Sticky Trap ◽

Yellow Leaf ◽

Trap Plate ◽

Dna Extraction Method ◽

Yellow Sticky Trap ◽

Leaf Curl Virus ◽

Non Destructive

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A rapid non-destructive DNA extraction method for insects and other arthropods

Journal of Asia-Pacific Entomology ◽

10.1016/j.aspen.2010.04.003 ◽

2010 ◽

Vol 13 (3) ◽

pp. 243-248 ◽

Cited By ~ 38

Author(s):

Mark A. Castalanelli ◽

Dustin L. Severtson ◽

Cameron J. Brumley ◽

Andras Szito ◽

Robert G. Foottit ◽

...

Keyword(s):

Dna Extraction ◽

Extraction Method ◽

Dna Extraction Method ◽

Non Destructive

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An efficient and non-destructive DNA extraction method for oribatid mites

Archives of Phytopathology and Plant Protection ◽

10.1080/03235408.2018.1444003 ◽

2018 ◽

Vol 51 (3-4) ◽

pp. 187-196 ◽

Cited By ~ 1

Author(s):

Mansoureh Ahaniazad ◽

Mohammad Bagheri ◽

Vahid Roumi ◽

Mohammad Ali Akrami

Keyword(s):

Dna Extraction ◽

Extraction Method ◽

Oribatid Mites ◽

Dna Extraction Method ◽

Non Destructive

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Sensitivity detection of Abacavir in human through SNP detection of HLA-B*5701 allele

International Journal of Bioassays ◽

10.21746/ijbio.2016.08.006 ◽

2016 ◽

Vol 5 (08) ◽

pp. 4754

Author(s):

Tanushree Mitra* ◽

Shivshankar Kumdale ◽

Sameer Chowdhary ◽

Amol D. Raut

Keyword(s):

Blood Sample ◽

Dna Extraction ◽

Genomic Dna ◽

Extraction Method ◽

Snp Detection ◽

Dna Extraction Method ◽

Sensitivity Detection

The main objective of this study was to make sure whether randomly taken 12 samples were sensitive to abacavir. The genomic DNA from 12 blood sample were extracted by phenol chloroform DNA extraction method, extracted genomic DNA were amplified and sequenced, thereafter SNPs were detected. Every sample had shown the presence of normal base at SNP position. This study indicated, those randomly taken 12 patients were sensitive to abacavir, so they can consume abacavir if they get infected with HIV.

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