Peer Review #1 of "The effects of resveratrol feeding and exercise training on the skeletal muscle function and transcriptome of aged rats (v0.2)"

This study investigated the effects of resveratrol feeding and exercise training on the skeletal muscle function and transcriptome of aged rats. Male SD rats (25 months old) were divided into the control group (Old), the daily exercise training group (Trained), and the resveratrol feeding group (Resveratrol). After 6 weeks of intervention, the body mass, grip strength, and gastrocnemius muscle mass were determined, and the muscle samples were analyzed by transcriptome sequencing. The differentially expressed genes were analyzed followed by GO enrichment analysis and KEGG analysis. The Old group showed positive increases in body mass, while both the Trained and Resveratrol groups showed negative growth. No significant differences in the gastrocnemius muscle index and absolute grip strength were found among the three groups. However, the relative grip strength was higher in the Trained group than in the Old group. Only 21 differentially expressed genes were identified in the Trained group vs. the Old group, and 12 differentially expressed genes were identified in the Resveratrol group vs. the Old group. The most enriched GO terms in the Trained group vs. the Old group were mainly associated with RNA metabolic processes and transmembrane transporters, and the significantly upregulated KEGG pathways included mucin-type O-glycan biosynthesis, drug metabolism, and pyrimidine metabolism. The most enriched GO terms in the Resveratrol group vs. the Old group were primarily associated with neurotransmitter transport and synaptic vesicle, and the upregulated KEGG pathways included synaptic vesicle cycle, nicotine addiction, retinol metabolism, insulin secretion, retrograde endocannabinoid signaling, and glutamatergic synapse. Neither exercise training nor resveratrol feeding has a notable effect on skeletal muscle function and related gene expression in aged rats. However, both exercise training and resveratrol feeding have strong effects on weight loss, which is beneficial for reducing the exercise loads of the elderly.

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Relative effects of exercise training and alendronate treatment on skeletal muscle function of ovariectomized rats

Menopause The Journal of The North American Menopause Society ◽

10.1097/01.gme.0000227861.35226.fa ◽

2007 ◽

Vol 14 (3) ◽

pp. 528-534 ◽

Cited By ~ 12

Author(s):

Jeffrey J. Widrick ◽

Robyn Fuchs ◽

Gianni F. Maddalozzo ◽

Kevin Marley ◽

Christine Snow

Keyword(s):

Skeletal Muscle ◽

Exercise Training ◽

Muscle Function ◽

Ovariectomized Rats ◽

Skeletal Muscle Function ◽

Alendronate Treatment

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Effects of moderate heart failure and functional overload on rat plantaris muscle

Journal of Applied Physiology ◽

10.1152/jappl.2002.92.1.18 ◽

2002 ◽

Vol 92 (1) ◽

pp. 18-24 ◽

Cited By ~ 8

Author(s):

Espen E. Spangenburg ◽

Simon J. Lees ◽

Jeff S. Otis ◽

Timothy I. Musch ◽

Robert J. Talmadge ◽

...

Keyword(s):

Heart Failure ◽

Skeletal Muscle ◽

Exercise Training ◽

Muscle Function ◽

Skeletal Muscle Function ◽

Moderate Heart Failure ◽

Plasmic Reticulum ◽

Uptake Rates ◽

Isoform Expression ◽

And Control

It is thought that changes in sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) of skeletal muscle contribute to alterations in skeletal muscle function during congestive heart failure (CHF). It is well established that exercise training can improve muscle function. However, it is unclear whether similar adaptations will result from exercise training in a CHF patient. Therefore, the purpose of this study was to determine whether skeletal muscle during moderate CHF adapts to increased activity, utilizing the functional overload (FO) model. Significant increases in plantaris mass of the CHF-FO and sham-FO groups compared with the CHF and control (sham) groups were observed. Ca2+ uptake rates were significantly elevated in the CHF group compared with all other groups. No differences were detected in Ca2+ uptake rates between the CHF-FO, sham, and sham-FO groups. Increases in Ca2+ uptake rates in moderate-CHF rats were not due to changes in SERCA isoform proportions; however, FO may have attenuated the CHF-induced increases through alterations in SERCA isoform expression. Therefore, changes in skeletal muscle Ca2+handling during moderate CHF may be due to alterations in regulatory mechanisms, which exercise may override, by possibly altering SERCA isoform expression.

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Aerobic exercise training improves skeletal muscle function and Ca2+ handling-related protein expression in sympathetic hyperactivity-induced heart failure

Journal of Applied Physiology ◽

10.1152/japplphysiol.00281.2010 ◽

2010 ◽

Vol 109 (3) ◽

pp. 702-709 ◽

Cited By ~ 38

Author(s):

C. R. Bueno ◽

J. C. B. Ferreira ◽

M. G. Pereira ◽

A. V. N. Bacurau ◽

P. C. Brum

Keyword(s):

Skeletal Muscle ◽

Exercise Training ◽

Protein Expression ◽

Aerobic Exercise ◽

Muscle Function ◽

Exercise Tolerance ◽

Aerobic Exercise Training ◽

Related Protein ◽

Skeletal Muscle Function ◽

Related Proteins

The cellular mechanisms of positive effects associated with aerobic exercise training on overall intrinsic skeletal muscle changes in heart failure (HF) remain unclear. We investigated potential Ca2+ abnormalities in skeletal muscles comprising different fiber compositions and investigated whether aerobic exercise training would improve muscle function in a genetic model of sympathetic hyperactivity-induced HF. A cohort of male 5-mo-old wild-type (WT) and congenic α2A/α2C adrenoceptor knockout (ARKO) mice in a C57BL/6J genetic background were randomly assigned into untrained and trained groups. Exercise training consisted of a 8-wk running session of 60 min, 5 days/wk (from 5 to 7 mo of age). After completion of the exercise training protocol, exercise tolerance was determined by graded treadmill exercise test, muscle function test by Rotarod, ambulation and resistance to inclination tests, cardiac function by echocardiography, and Ca2+ handling-related protein expression by Western blot. α2A/α2CARKO mice displayed decreased ventricular function, exercise intolerance, and muscle weakness paralleled by decreased expression of sarcoplasmic Ca2+ release-related proteins [α1-, α2-, and β1-subunits of dihydropyridine receptor (DHPR) and ryanodine receptor (RyR)] and Ca2+ reuptake-related proteins [sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA)1/2 and Na+/Ca2+ exchanger (NCX)] in soleus and plantaris. Aerobic exercise training significantly improved exercise tolerance and muscle function and reestablished the expression of proteins involved in sarcoplasmic Ca2+ handling toward WT levels. We provide evidence that Ca2+ handling-related protein expression is decreased in this HF model and that exercise training improves skeletal muscle function associated with changes in the net balance of skeletal muscle Ca2+ handling proteins.

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