scholarly journals Genetic diversity and population structure of Chinese jujube (Ziziphus jujuba Mill.) and sour jujube (Ziziphus acidojujuba Mill.) using inter-simple sequence repeat (ISSR) Markers

2018 ◽  
Author(s):  
Shipeng Li ◽  
Mingxin Guo ◽  
Pengcheng Fu ◽  
Hongxia Liu ◽  
Xusheng Zhao
Keyword(s):  
Population Structure ◽  
Germplasm Conservation ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Chinese Jujube ◽  
Population Structure Analysis ◽  
Conservation Genetic ◽  
Ziziphus Jujuba ◽  
Simple Sequence ◽  
Issr Primers

The Chinese jujube (Ziziphus jujuba Mill.) originates from sour jujube (Ziziphus acidojujuba Mill.) and is an economically important genus in the Rhamnaceae family. However, little is known about the genetic relationship between jujube cultivars and wild species. In this study, we estimated the genetic variation and relationships between 85 jujube cultivars and 55 sour jujube individuals by ISSR markers. Of 216 ISSR primers, 110 were able produce amplified product(s) and 28 showed polymorphisms, accounting for 50.9% and 25.5% of total primers respectively. A total of 89 loci were amplified with 28 primers, of which 42 loci (47.2%) were polymorphic, and most of primers exhibited highly PIC values. Cluster analysis and population structure analysis roughly divided the 140 accessions into two major groups. One group included all jujube cultivars and some sour jujube individuals, and the other group included remaining sour jujube individuals. Most jujube cultivars have a certain correlation with their origin, and there are obvious gene exchanges between sour jujube and jujube cultivars. The results provide a useful basis for jujube germplasm conservation, genetic improvement and evolution research.

scholarly journals Genetic diversity and population structure of Chinese jujube (Ziziphus jujuba Mill.) and sour jujube (Ziziphus acidojujuba Mill.) using inter-simple sequence repeat (ISSR) Markers

2018 ◽  
Author(s):  
Shipeng Li ◽  
Mingxin Guo ◽  
Pengcheng Fu ◽  
Hongxia Liu ◽  
Xusheng Zhao
Keyword(s):  
Population Structure ◽  
Germplasm Conservation ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Chinese Jujube ◽  
Population Structure Analysis ◽  
Conservation Genetic ◽  
Ziziphus Jujuba ◽  
Simple Sequence ◽  
Issr Primers

The Chinese jujube (Ziziphus jujuba Mill.) originates from sour jujube (Ziziphus acidojujuba Mill.) and is an economically important genus in the Rhamnaceae family. However, little is known about the genetic relationship between jujube cultivars and wild species. In this study, we estimated the genetic variation and relationships between 85 jujube cultivars and 55 sour jujube individuals by ISSR markers. Of 216 ISSR primers, 110 were able produce amplified product(s) and 28 showed polymorphisms, accounting for 50.9% and 25.5% of total primers respectively. A total of 89 loci were amplified with 28 primers, of which 42 loci (47.2%) were polymorphic, and most of primers exhibited highly PIC values. Cluster analysis and population structure analysis roughly divided the 140 accessions into two major groups. One group included all jujube cultivars and some sour jujube individuals, and the other group included remaining sour jujube individuals. Most jujube cultivars have a certain correlation with their origin, and there are obvious gene exchanges between sour jujube and jujube cultivars. The results provide a useful basis for jujube germplasm conservation, genetic improvement and evolution research.

scholarly journals Screening of homokaryotic protoclones of Agaricus bisporus (J. Lge) Imbach by colony characters and ISSR markers

2010 ◽  
Vol 39 (1) ◽  
pp. 119-122 ◽  
Author(s):  
Mahmudul Islam Nazrul ◽  
Fan Xiao Lin ◽  
Bian Yin-Bing
Keyword(s):  
Simple Sequence Repeat ◽  
Agaricus Bisporus ◽  
Morphological Characters ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Fruiting Bodies ◽  
Sequence Repeat ◽  
Slow Growing ◽  
Simple Sequence ◽  
Issr Primers

Among ten slow-growing protoclones of Agaricus bisporus (J. Lge) Imbach, all appressed colonies showed slower growth rate and spawn run, and inability to produce fruiting bodies in substrate. Seven of 40 inter-simple sequence repeat (ISSR) primers amplified 78 reproducible fragments, 48.93% were polymorphic, each producing 7 to 16 bands ranging from 0.10 to 2.10 kbp, sufficient to differentiate the protoclones from each other. Appressed protoclones were homoallelic at a number of loci that were heteroallelic in the parent, suggesting that they represented rare homokaryons. Thus, using morphological characters along with ISSR, polymorphisms could be useful for quick, easy, and accurate in distinguishing homo- and heterokaryotic isolates. Key words: Agaricus bisporus (J. Lge) Imbach; Homokaryon; ISSR; Protoclone DOI: 10.3329/bjb.v39i1.5537Bangladesh J. Bot. 39(1): 119-122, 2010 (June)

scholarly journals Informative Inter Simple Sequence Repeat (ISSR) primers for genetic analysis of coconut (Cocos nucifera L.) germplasm

CORD ◽  
2005 ◽  
Vol 21 (02) ◽  
pp. 34 ◽  
Author(s):  
R. Manimekalai
Keyword(s):  
Information Content ◽  
Polymorphism Information Content ◽  
Simple Sequence Repeat ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Rapid Screening ◽  
Sequence Repeat ◽  
Marker Index ◽  
Simple Sequence ◽  
Issr Primers

Inter Simple Sequence Repeat (ISSR) markers are versatile and used in a number of applications viz. genetic diversity estimation, phylogenetic relationship and gene tagging in different crops. In coconut, the ISSR markers are being reported here for the first time. In the present paper, thirty-five  primers targeting to amplify the inter microsatellite  regions were screened  using  thirty  different  coconut  germplasm  accessions.  The  ISSR  primers  were evaluated based on polymorphism information content and marker index.  Out of 35 primers screened,  19 primers  produced  clear amplification  pattern.  The polymorphism  information content varied between 0.019 and 0.386, whereas, the marker index ranged from 0.019 to 5.673  among  the  primers.    Based  on  the  high  marker  index,  five  and  ten  primers  were selected.  The similarity  matrices  were constructed  separately  for five, ten and 19 primers using NYSYS software and the correlation was tested using Mantel’s test. There was high correlation  between five and ten primers with 19 primers. Hence, the primers with higher marker index (5 and 10 primers) were regarded as informative  primers. These informative primers can be used to develop more polymorphic markers in coconut for rapid screening of germplasm materials.

Genetic diversity in wild Lens spp. using inter simple sequence repeat (ISSR) marker

2015 ◽  
Vol 38 (5) ◽  
Author(s):  
Padmavati G. Gore ◽  
M. K. Rana ◽  
Kuldeep Tripathi ◽  
Mohar Singh ◽  
I. S. Bisht ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeat ◽  
Molecular Diversity ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Sequence Repeat ◽  
Principal Coordinates ◽  
Cultivated Species ◽  
Simple Sequence ◽  
Issr Primers

Genetic diversity was assessed in 50 accessions of seven <italic>Lens</italic> species using ISSR markers. The collection included accessions of the cultivated species <italic>L. culinaris</italic> and six wild species, <italic>viz</italic>., <italic>L. culinaris</italic> ssp. <italic>odemensis, L. culinaris</italic> ssp. <italic>orientalis</italic>, <italic>L.</italic> <italic>orientalis, L. nigricans, L. lamottei</italic> and <italic>L. ervoides.</italic> The 23 ISSR primers amplified a total of 368 bands with an average of 16 bands per primer. Maximum number of 20 bands was amplified using each of the primers ISSR-34 and ISSR-835. All the primers were found to be polymorphic. PIC values ranged from 0.02 to 0.80. The primers ISSR-807, ISSR- 809, ISSR- 827, ISSR- 847, ISSR-28 and ISSR- 37 were found to be very useful for analyzing the molecular diversity of the genus <italic>Lens</italic>. Cluster Analysis and Principal Coordinates Analyses placed the 50 accessions into two groups and complemented each other.

Genetic variability of Orobanche aegyptiaca infesting tobacco in Iran by Bayesian analysis

Biologia ◽  
2014 ◽  
Vol 69 (12) ◽  
Author(s):  
Samaneh Abedi ◽  
Reza Darvishzadeh ◽  
Iraj Bernousi ◽  
Babak Abdollahi Mandoulakani ◽  
Hamid Hatami Maleki ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Similarity Coefficients ◽  
Genetic Groups ◽  
Wide Range ◽  
Orobanche Aegyptiaca ◽  
Simple Sequence ◽  
Issr Primers

AbstractBroomrapes (Orobanche L.) are holoparasitic plants, parasitizing roots of a wide range of host plants. In this study, genetic polymorphism among 44 Orobanche aegyptiaca Pers. individuals collected from different regions of northwest Iran was investigated using inter-simple sequence repeat (ISSR) markers. Two hundred-sixty one discernible bands were amplified using 20 ISSR primers which 245 (94%) was polymorphic, indicating considerable genetic variation among the examined individuals. The number of polymorphic bands per primer ranged from 4 to 17, averaging 12.25. UPGMA clustering using Jaccard’s similarity coefficients revealed six main groups. Genetic similarity coefficients varied from 0.71 (between individuals 23 and 27) to 0.34 (between 13 and 30). A model-based Bayesian approach subdivided 38 out of 44 broomrape genotypes into 2 genetic groups and the remaining ones were categorized as mixed genotypes based on Q values. According to an analysis of molecular variance, 99% of the total variation was partitioned within genetic groups. The results demonstrated the potential usefulness of ISSR markers for determination of genetic variation in O. aegyptiaca. Understanding the variability in broomrape is important when attempting to develop resistant host crops against this parasite.

scholarly journals Association of Morphological, Ecological, and Genetic Diversity of Aerva javanica Populations Growing in the Eastern Desert of Egypt

Agronomy ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 402
Author(s):  
Noha A. El-Tayeh ◽  
Hanaa K. Galal ◽  
Magda I. Soliman ◽  
Hoida Zaki
Keyword(s):  
Morphological Diversity ◽  
Eastern Desert ◽  
Molecular Data ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Soil Variables ◽  
Sustainable Conservation ◽  
Simple Sequence ◽  
Important Medicinal Plant ◽  
Issr Primers

Aerva javanica is one of Egypt’s most important traditional medicinal plants used as antidiarrheal and anthelmintic medicine and recently as an anticancer agent. In this study, variations among ten populations of Aerva javanica in different sites in the Eastern Desert of Egypt were analyzed based on morphological and ecological attributes and molecular variation expressed by Inter-Simple Sequence Repeat (ISSR) markers. Morphological diversity was higher for populations in the Wadi El-Markh and Bir Abbady regions than others. The polymorphism revealed by ten ISSR primers was 79.4% among populations. Distance trees created using the results obtained from soil variables, morphological characterizations, and molecular data showed that the highest similarity was 0.974 between Populations 8 and 9, while the lowest similarity was 0.715 between Population 1 and Population 3 regions. In conclusion, the obtained data are important to design a plan for sustainable conservation of Aerva javanica as an important medicinal plant having a wide interspecific genetic variability within various populations.

scholarly journals Molecular Distinction Among Mulberry (Morus Spp.) Species and Varieties Cultivated in DPR Korea

2021 ◽  
Author(s):  
Un-Hyang Ho ◽  
Jung Sam Kye ◽  
Song Im Chae ◽  
Jong Ho Kim ◽  
Myong Ho Kim
Keyword(s):  
Genetic Variation ◽  
Internal Transcribed Spacer ◽  
Simple Sequence Repeat ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Breeding Strategy ◽  
Issr Analysis ◽  
Morus Spp ◽  
Simple Sequence ◽  
Issr Primers

Abstract Mulberry (Morus spp.) is a cross-pollinating and highly hybridized plant of which productivity are greatly varied in different varieties. We analysed molecular distinction among four mulberry species and varieties cultivated in DPR Korea by using nuclear ribosomal internal transcribed spacer (ITS) sequences and inter simple sequence repeat (ISSR) markers. ITS sequences didn’t represent a remarkable interspecific distinction among four mulberry species used in our study, suggesting that it could not be employed to identify them. ISSR analysis using 16 random primers generated 158 different markers ranging from 100 to 4000 bp in size. The results showed the inter-specific genetic variation (55.34%) was slightly higher than intra-specific genetic variation(44.66%), with comparatively low average number of migrants per generation (Nm) among populations (0.3886). Using ISSR primers selected in this study, in the future, the suitable breeding strategy might be established in raising of elite mulberry varieties on the basis of interspecific hybridization.

scholarly journals Assessment of somaclonal variation in indirect morphogenesis-derived plants of Arracacia xanthorrhiza

2019 ◽  
Vol 54 ◽  
Author(s):  
Jan Vitamvas ◽  
Iva Viehmannova ◽  
Petra Hlasna Cepkova ◽  
Hana Mrhalova ◽  
Katerina Eliasova
Keyword(s):  
Somaclonal Variation ◽  
Simple Sequence Repeat ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Half Strength ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Simple Sequence ◽  
Issr Primers

Abstract: The objective of this work was to induce and detect somaclonal variation in arracacha (Arracacia xanthorrhiza) plants regenerated via indirect morphogenesis, in order to evaluate the potential of this technique to produce new genotypes for breeding purposes of this crop. Calli were induced from petiole segments on Murashige & Skoog (MS) medium supplied with 0.1 mg L-1 2,4-dichlorophenoxyacetic acid. The regeneration of plants via indirect morphogenesis was carried out on half-strength MS medium without plant growth regulators. Fifteen randomly chosen plants were subjected to flow cytometry and “inter-simple sequence repeat” (ISSR) analysis. Ploidy level remained stable in all tested regenerants (2n=4x=44), with no changes in the genome. Eighteen ISSR primers produced a total of 1,584 fragments in all samples. Two ISSR primers produced four polymorphic fragments in 26.7% of the tested samples. Somaclonal variation in arracacha is a result of plant regeneration via indirect morphogenesis and can be detected by ISSR markers.

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