scholarly journals Complete genome sequence of new virulent actinophages belonging to Streptomyces flavovirens

2018 ◽  
Author(s):  
Abdoallah Sharaf ◽  
Francesco Mercati ◽  
Ibrahim Elmaghraby ◽  
Reham Elbaz ◽  
Eman Marei
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Genomic Analysis ◽  
Comparative Genomic ◽  
Putative Orfs ◽  
Phage Group ◽  
Significant Homology ◽  
Egyptian Soil ◽  
Adsorption Rates

Complete genome sequence of new virulent actinophages belonging to Streptomyces flavovirens We report biological and physiochemical features and use NGS to provide the complete annotated genomes for two new strains (Sf1 and Sf3) of the virulent phage Streptomyces flavovirens, isolated from Egyptian soil samples. The S. flavovirens phages (Sf1 and Sf3) show high adsorption rates (82 and 85 %, respectively), indicating a strong specificity to their host, and their burst sizes were 1.95 and 2.49 virions per mL. The phage genomes are parts of a singleton cluster with sizes of 43,150 bp and 60,934 bp, respectively. the assignment of possible functions to 19 and 28 putative ORFs were identified, which included phage structural proteins, lysis components and metabolic proteins. Comparative genomic analysis revealed significant homology between the two phages and the closest Streptomyces phage (VWB phages). However, the phylogenetic analysis highlighted that the isolated phages belong to the BG Streptomyces phage group but are clearly separated, representing a novel sub-cluster.

scholarly journals Complete genome sequence of new virulent actinophages belonging to Streptomyces flavovirens

2018 ◽  
Author(s):  
Abdoallah Sharaf ◽  
Francesco Mercati ◽  
Ibrahim Elmaghraby ◽  
Reham Elbaz ◽  
Eman Marei
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Genomic Analysis ◽  
Comparative Genomic ◽  
Putative Orfs ◽  
Phage Group ◽  
Significant Homology ◽  
Egyptian Soil ◽  
Adsorption Rates

Complete genome sequence of new virulent actinophages belonging to Streptomyces flavovirens We report biological and physiochemical features and use NGS to provide the complete annotated genomes for two new strains (Sf1 and Sf3) of the virulent phage Streptomyces flavovirens, isolated from Egyptian soil samples. The S. flavovirens phages (Sf1 and Sf3) show high adsorption rates (82 and 85 %, respectively), indicating a strong specificity to their host, and their burst sizes were 1.95 and 2.49 virions per mL. The phage genomes are parts of a singleton cluster with sizes of 43,150 bp and 60,934 bp, respectively. the assignment of possible functions to 19 and 28 putative ORFs were identified, which included phage structural proteins, lysis components and metabolic proteins. Comparative genomic analysis revealed significant homology between the two phages and the closest Streptomyces phage (VWB phages). However, the phylogenetic analysis highlighted that the isolated phages belong to the BG Streptomyces phage group but are clearly separated, representing a novel sub-cluster.

scholarly journals Complete Genome Sequence of Streptococcus mutans 27-3, an Active Extracellular Membrane Vesicle Producer

2021 ◽  
Vol 10 (25) ◽  
Author(s):  
Xiaochang Huang ◽  
Justin Merritt ◽  
Zezhang Tom Wen
Keyword(s):  
Streptococcus Mutans ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Membrane Vesicle ◽  
Genomic Analysis ◽  
Membrane Vesicles ◽  
Laboratory Strain ◽  
Comparative Genomic ◽  
Content Type

Here, we report the complete genome sequence of Streptococcus mutans 27-3. Isolated from a caries-active patient, 27-3 produces significantly more extracellular membrane vesicles than the commonly used laboratory strain UA159. This study provides useful information for comparative genomic analysis and better understanding of regulation of vesiculogenesis in this bacterium.

scholarly journals Complete genome sequence of Enterococcus faecium strain TX16 and comparative genomic analysis of Enterococcus faecium genomes

2012 ◽  
Vol 12 (1) ◽  
pp. 135 ◽  
Author(s):  
Xiang Qin ◽  
Jessica R Galloway-Peña ◽  
Jouko Sillanpaa ◽  
Jung Roh ◽  
Sreedhar R Nallapareddy ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Enterococcus Faecium ◽  
Complete Genome ◽  
Genomic Analysis ◽  
Comparative Genomic Analysis ◽  
Comparative Genomic ◽  
Faecium Strain

scholarly journals Complete Genome Sequence of Sinorhizobium Phage ΦM6, the First Terrestrial Phage of a Marine Phage Group

2018 ◽  
Vol 7 (13) ◽  
Author(s):  
Tess E. Brewer ◽  
Brian K. Washburn ◽  
Jason S. Lynn ◽  
Kathryn M. Jones
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Sinorhizobium Meliloti ◽  
Nitrogen Fixing ◽  
Phage Group ◽  
Content Type ◽  
Marine Phage

Sinorhizobium phage ΦM6 infects the nitrogen-fixing rhizobial bacterium Sinorhizobium meliloti. ΦM6 most closely resembles marine phages, such as Puniceispirillum phage HMO-2011, rather than previously sequenced rhizobial phages.

scholarly journals Complete Genome Sequence of Streptococcus mutans Strain MD, Which Produces Highly Potent Mutacins

2020 ◽  
Vol 9 (33) ◽  
Author(s):  
Saswati Biswas ◽  
Indranil Biswas
Keyword(s):  
Streptococcus Mutans ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Comparative Genomic ◽  
Content Type ◽  
Genomic Study ◽  
Comparative Genomic Study ◽  
Genome Information

ABSTRACT Here, we report the complete genome sequence of Streptococcus mutans strain MD, which produces potent mutacins capable of inhibiting streptococci. MD is a relatively uncharacterized strain whose genome information was unavailable. This study provides useful information for comparative genomic study and for understanding the repertoire of mutacins in S. mutans.

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