scholarly journals Complete Genome Sequence of Weissella hellenica 0916-4-2 and Its Comparative Genomic Analysis

2019 ◽  
Vol 10 ◽  
Author(s):  
Suresh Panthee ◽  
Atmika Paudel ◽  
Jochen Blom ◽  
Hiroshi Hamamoto ◽  
Kazuhisa Sekimizu
2012 ◽  
Vol 12 (1) ◽  
pp. 135 ◽  
Author(s):  
Xiang Qin ◽  
Jessica R Galloway-Peña ◽  
Jouko Sillanpaa ◽  
Jung Roh ◽  
Sreedhar R Nallapareddy ◽  
...  

2021 ◽  
Vol 10 (25) ◽  
Author(s):  
Xiaochang Huang ◽  
Justin Merritt ◽  
Zezhang Tom Wen

Here, we report the complete genome sequence of Streptococcus mutans 27-3. Isolated from a caries-active patient, 27-3 produces significantly more extracellular membrane vesicles than the commonly used laboratory strain UA159. This study provides useful information for comparative genomic analysis and better understanding of regulation of vesiculogenesis in this bacterium.


2011 ◽  
Vol 193 (22) ◽  
pp. 6342-6357 ◽  
Author(s):  
Neha Jalan ◽  
Valente Aritua ◽  
Dibyendu Kumar ◽  
Fahong Yu ◽  
Jeffrey B. Jones ◽  
...  

Xanthomonas axonopodispv. citrumelo is a citrus pathogen causing citrus bacterial spot disease that is geographically restricted within the state of Florida. Illumina, 454 sequencing, and optical mapping were used to obtain a complete genome sequence ofX. axonopodispv. citrumelo strain F1, 4.9 Mb in size. The strain lacks plasmids, in contrast to other citrusXanthomonaspathogens. Phylogenetic analysis revealed that this pathogen is very close to the tomato bacterial spot pathogenX. campestrispv. vesicatoria 85-10, with a completely different host range. We also comparedX. axonopodispv. citrumelo to the genome of citrus canker pathogenX. axonopodispv. citri 306. Comparative genomic analysis showed differences in several gene clusters, like those for type III effectors, the type IV secretion system, lipopolysaccharide synthesis, and others. In addition topthA, effectors such asxopE3, xopAI, andhrpWwere absent fromX. axonopodispv. citrumelo while present inX. axonopodispv. citri. These effectors might be responsible for survival and the low virulence of this pathogen on citrus compared to that ofX. axonopodispv. citri. We also identified unique effectors inX. axonopodispv. citrumelo that may be related to the different host range as compared to that ofX. axonopodispv. citri.X. axonopodispv. citrumelo also lacks various genes, such assyrE1, syrE2, and RTX toxin family genes, which were present inX. axonopodispv. citri. These may be associated with the distinct virulences ofX. axonopodispv. citrumelo andX. axonopodispv. citri. Comparison of the complete genome sequence ofX. axonopodispv. citrumelo to those ofX. axonopodispv. citri andX. campestrispv. vesicatoria provides valuable insights into the mechanism of bacterial virulence and host specificity.


2018 ◽  
Author(s):  
Abdoallah Sharaf ◽  
Francesco Mercati ◽  
Ibrahim Elmaghraby ◽  
Reham Elbaz ◽  
Eman Marei

Complete genome sequence of new virulent actinophages belonging to Streptomyces flavovirens We report biological and physiochemical features and use NGS to provide the complete annotated genomes for two new strains (Sf1 and Sf3) of the virulent phage Streptomyces flavovirens, isolated from Egyptian soil samples. The S. flavovirens phages (Sf1 and Sf3) show high adsorption rates (82 and 85 %, respectively), indicating a strong specificity to their host, and their burst sizes were 1.95 and 2.49 virions per mL. The phage genomes are parts of a singleton cluster with sizes of 43,150 bp and 60,934 bp, respectively. the assignment of possible functions to 19 and 28 putative ORFs were identified, which included phage structural proteins, lysis components and metabolic proteins. Comparative genomic analysis revealed significant homology between the two phages and the closest Streptomyces phage (VWB phages). However, the phylogenetic analysis highlighted that the isolated phages belong to the BG Streptomyces phage group but are clearly separated, representing a novel sub-cluster.


2018 ◽  
Author(s):  
Abdoallah Sharaf ◽  
Francesco Mercati ◽  
Ibrahim Elmaghraby ◽  
Reham Elbaz ◽  
Eman Marei

Complete genome sequence of new virulent actinophages belonging to Streptomyces flavovirens We report biological and physiochemical features and use NGS to provide the complete annotated genomes for two new strains (Sf1 and Sf3) of the virulent phage Streptomyces flavovirens, isolated from Egyptian soil samples. The S. flavovirens phages (Sf1 and Sf3) show high adsorption rates (82 and 85 %, respectively), indicating a strong specificity to their host, and their burst sizes were 1.95 and 2.49 virions per mL. The phage genomes are parts of a singleton cluster with sizes of 43,150 bp and 60,934 bp, respectively. the assignment of possible functions to 19 and 28 putative ORFs were identified, which included phage structural proteins, lysis components and metabolic proteins. Comparative genomic analysis revealed significant homology between the two phages and the closest Streptomyces phage (VWB phages). However, the phylogenetic analysis highlighted that the isolated phages belong to the BG Streptomyces phage group but are clearly separated, representing a novel sub-cluster.


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