scholarly journals Complete Genome Sequence of Streptococcus mutans 27-3, an Active Extracellular Membrane Vesicle Producer

2021 ◽  
Vol 10 (25) ◽  
Author(s):  
Xiaochang Huang ◽  
Justin Merritt ◽  
Zezhang Tom Wen
Keyword(s):  
Streptococcus Mutans ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Membrane Vesicle ◽  
Genomic Analysis ◽  
Membrane Vesicles ◽  
Laboratory Strain ◽  
Comparative Genomic ◽  
Content Type

Here, we report the complete genome sequence of Streptococcus mutans 27-3. Isolated from a caries-active patient, 27-3 produces significantly more extracellular membrane vesicles than the commonly used laboratory strain UA159. This study provides useful information for comparative genomic analysis and better understanding of regulation of vesiculogenesis in this bacterium.

scholarly journals Complete Genome Sequence of Streptococcus mutans Strain MD, Which Produces Highly Potent Mutacins

2020 ◽  
Vol 9 (33) ◽  
Author(s):  
Saswati Biswas ◽  
Indranil Biswas
Keyword(s):  
Streptococcus Mutans ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Comparative Genomic ◽  
Content Type ◽  
Genomic Study ◽  
Comparative Genomic Study ◽  
Genome Information

ABSTRACT Here, we report the complete genome sequence of Streptococcus mutans strain MD, which produces potent mutacins capable of inhibiting streptococci. MD is a relatively uncharacterized strain whose genome information was unavailable. This study provides useful information for comparative genomic study and for understanding the repertoire of mutacins in S. mutans.

scholarly journals Composite Long- and Short-Read Sequencing Delivers a Complete Genome Sequence of B04Sm5, a Reutericyclin- and Mutanocyclin-Producing Strain of Streptococcus mutans

2020 ◽  
Vol 9 (47) ◽  
Author(s):  
Jonathon L. Baker ◽  
Anna Edlund
Keyword(s):  
Streptococcus Mutans ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Biosynthetic Gene Cluster ◽  
Commensal Bacteria ◽  
Biosynthetic Gene ◽  
Tetramic Acid ◽  
Content Type ◽  
Short Read Sequencing

ABSTRACT Streptococcus mutans strain B04Sm5 was recently shown to inhibit the growth of neighboring commensal bacteria using reutericyclin, an acylated tetramic acid produced by the muc biosynthetic gene cluster. Here, a complete genome sequence of B04Sm5 is reported.

scholarly journals Biology and Genome Sequence of Streptococcus mutans Phage M102AD

2012 ◽  
Vol 78 (7) ◽  
pp. 2264-2271 ◽  
Author(s):  
Allan L. Delisle ◽  
Ming Guo ◽  
Natalia I. Chalmers ◽  
Gerard J. Barcak ◽  
Geneviève M. Rousseau ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Genome Sequence ◽  
Gc Content ◽  
Genomic Analysis ◽  
Streptococcus Thermophilus ◽  
Open Reading Frames ◽  
Comparative Genomic ◽  
Content Type ◽  
Close Relationship ◽  
Virion Structure

ABSTRACTM102AD is the new designation for aStreptococcus mutansphage described in 1993 as phage M102. This change was necessitated by the genome analysis of anotherS. mutansphage named M102, which revealed differences from the genome sequence reported here. Additional host range analyses confirmed thatS. mutansphage M102AD infects only a few serotype c strains. Phage M102AD adsorbed very slowly to its host, and it cannot adsorb to serotype e and f strains ofS. mutans. M102AD adsorption was blocked by c-specific antiserum. Phage M102AD also adsorbed equally well to heat-treated and trypsin-treated cells, suggesting carbohydrate receptors. Saliva and polysaccharide production did not inhibit plaque formation. The genome of this siphophage consisted of a linear, double-stranded, 30,664-bp DNA molecule, with a GC content of 39.6%. Analysis of the genome extremities indicated the presence of a 3′-overhangcossite that was 11 nucleotides long. Bioinformatic analyses identified 40 open reading frames, all in the same orientation. No lysogeny-related genes were found, indicating that phage M102AD is strictly virulent. No obvious virulence factor gene candidates were found. Twelve proteins were identified in the virion structure by mass spectrometry. Comparative genomic analysis revealed a close relationship betweenS. mutansphages M102AD and M102 as well as withStreptococcus thermophilusphages. This study also highlights the importance of conducting research with biological materials obtained from recognized microbial collections.

scholarly journals Complete Genome Sequence of Rhizobium japonicum Podophage Pasto

2021 ◽  
Vol 10 (3) ◽  
Author(s):  
Nikhil Manuel ◽  
Leika Rushing ◽  
Aravind Ravindran ◽  
Heather Newkirk ◽  
Ben Burrowes ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Genomic Analysis ◽  
Rhizobium Japonicum ◽  
Negative Bacterium ◽  
Significant Similarity ◽  
Gram Negative ◽  
Content Type

ABSTRACT Rhizobium japonicum is a Gram-negative bacterium of interest for research into nitrogen fixation in legumes. This article describes the isolation, sequencing, and annotation of R. japonicum podophage Pasto. While it shows no significant similarity to identified phages, genomic analysis indicates that Pasto may be temperate and is a novel T7-like podophage.

scholarly journals Complete Genome Sequence of Buttiauxella agrestis DSM 9389

2021 ◽  
Vol 10 (19) ◽  
Author(s):  
Nao Nakamichi ◽  
Ryota Moriuchi ◽  
Hideo Dohra ◽  
Hiroyuki Futamata ◽  
Yosuke Tashiro
Keyword(s):  
16S Rrna ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Membrane Vesicle ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Nucleotide Identity ◽  
Average Nucleotide Identity ◽  
Content Type

ABSTRACT We report here the complete genome sequence of Buttiauxella agrestis DSM 9389, which harbors eight 16S rRNA genes classified into three types. The genome sequence of this strain showed a high average nucleotide identity (97.3%) with that of the highly membrane vesicle-producing strain B. agrestis ATCC 33320T.

scholarly journals Complete Genome Sequence of Escherichia coli J53, an Azide-Resistant Laboratory Strain Used for Conjugation Experiments

2018 ◽  
Vol 6 (21) ◽  
Author(s):  
Yasufumi Matsumura ◽  
Gisele Peirano ◽  
Johann D. D. Pitout
Keyword(s):  
Escherichia Coli ◽  
Genetic Analysis ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Laboratory Strain ◽  
Content Type ◽  
E Coli ◽  
K 12

ABSTRACT We report here the complete genome sequence of Escherichia coli J53, which is used as a recipient in conjugation experiments and is a laboratory strain derived from E. coli K-12. This genome sequence will help in the development of a comprehensive genetic analysis of conjugative elements.

scholarly journals Complete Genome Sequence of Escherichia coli ME8067, an Azide-Resistant Laboratory Strain Used for Conjugation Experiments

2018 ◽  
Vol 6 (25) ◽  
Author(s):  
Yasufumi Matsumura ◽  
Masaki Yamamoto ◽  
Satoshi Nakano ◽  
Miki Nagao
Keyword(s):  
Escherichia Coli ◽  
Genetic Analysis ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Laboratory Strain ◽  
Content Type ◽  
E Coli ◽  
K 12

ABSTRACT We report here the complete genome sequence of Escherichia coli ME8067, an azide-resistant laboratory strain used for conjugation experiments. The ME8067 genome was closely related to E. coli strain K-12 substrain W3110. This genome sequence will support further genetic analysis of conjugative elements.

scholarly journals Complete Genome Sequence of Aeromonas rivipollensis KN-Mc-11N1, Isolated from a Wild Nutria (Myocastor coypus) in South Korea

2018 ◽  
Vol 7 (4) ◽  
Author(s):  
Seon Young Park ◽  
Se Ra Lim ◽  
Jee Soo Son ◽  
Hye Kwon Kim ◽  
Sun-Woo Yoon ◽  
...  
Keyword(s):  
South Korea ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Genomic Analysis ◽  
Zoonotic Potential ◽  
Myocastor Coypus ◽  
Content Type ◽  
Zoonotic Pathogens

We report here the complete genome sequence of Aeromonas rivipollensis KN-Mc-11N1, which was isolated from a wild nutria (Myocastor coypus) in South Korea. Genomic analysis indicated that A. rivipollensis may have zoonotic potential similar to that of other aeromonads, and nutria could be one of the sources of transmission of zoonotic pathogens to humans.

scholarly journals Comparative Genomic Analysis of Xanthomonas axonopodis pv. citrumelo F1, Which Causes Citrus Bacterial Spot Disease, and Related Strains Provides Insights into Virulence and Host Specificity

2011 ◽  
Vol 193 (22) ◽  
pp. 6342-6357 ◽  
Author(s):  
Neha Jalan ◽  
Valente Aritua ◽  
Dibyendu Kumar ◽  
Fahong Yu ◽  
Jeffrey B. Jones ◽  
...  
Keyword(s):  
Host Range ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Genomic Analysis ◽  
Comparative Genomic Analysis ◽  
Comparative Genomic ◽  
Bacterial Spot ◽  
Content Type ◽  
Xanthomonas Axonopodis ◽  
Bacterial Spot Disease

Xanthomonas axonopodispv. citrumelo is a citrus pathogen causing citrus bacterial spot disease that is geographically restricted within the state of Florida. Illumina, 454 sequencing, and optical mapping were used to obtain a complete genome sequence ofX. axonopodispv. citrumelo strain F1, 4.9 Mb in size. The strain lacks plasmids, in contrast to other citrusXanthomonaspathogens. Phylogenetic analysis revealed that this pathogen is very close to the tomato bacterial spot pathogenX. campestrispv. vesicatoria 85-10, with a completely different host range. We also comparedX. axonopodispv. citrumelo to the genome of citrus canker pathogenX. axonopodispv. citri 306. Comparative genomic analysis showed differences in several gene clusters, like those for type III effectors, the type IV secretion system, lipopolysaccharide synthesis, and others. In addition topthA, effectors such asxopE3, xopAI, andhrpWwere absent fromX. axonopodispv. citrumelo while present inX. axonopodispv. citri. These effectors might be responsible for survival and the low virulence of this pathogen on citrus compared to that ofX. axonopodispv. citri. We also identified unique effectors inX. axonopodispv. citrumelo that may be related to the different host range as compared to that ofX. axonopodispv. citri.X. axonopodispv. citrumelo also lacks various genes, such assyrE1, syrE2, and RTX toxin family genes, which were present inX. axonopodispv. citri. These may be associated with the distinct virulences ofX. axonopodispv. citrumelo andX. axonopodispv. citri. Comparison of the complete genome sequence ofX. axonopodispv. citrumelo to those ofX. axonopodispv. citri andX. campestrispv. vesicatoria provides valuable insights into the mechanism of bacterial virulence and host specificity.

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