10. Hypothesis Tests: Observed and Expected Values

Author(s):  
B. D. Athey ◽  
A. L. Stout ◽  
M. F. Smith ◽  
J. P. Langmore

Although there is general agreement that Inactive chromosome fibers consist of helically packed nucleosomes, the pattern of packing is still undetermined. Only one of the proposed models, the crossed-linker model, predicts a variable diameter dependent on the length of DNA between nucleosomes. Measurements of the fiber diameter of negatively-stained and frozen- hydrated- chromatin from Thyone sperm (87bp linker) and Necturus erythrocytes (48bp linker) have been previously reported from this laboratory. We now introduce a more reliable method of measuring the diameters of electron images of fibrous objects. The procedure uses a modified version of the computer program TOTAL, which takes a two-dimensional projection of the fiber density (represented by the micrograph itself) and projects it down the fiber axis onto one dimension. We illustrate this method using high contrast, in-focus STEM images of TMV and chromatin from Thyone and Necturus. The measured diameters are in quantitative agreement with the expected values for the crossed-linker model for chromatin structure


2019 ◽  
Author(s):  
Amanda Kay Montoya ◽  
Andrew F. Hayes

Researchers interested in testing mediation often use designs where participants are measured on a dependent variable Y and a mediator M in both of two different circumstances. The dominant approach to assessing mediation in such a design, proposed by Judd, Kenny, and McClelland (2001), relies on a series of hypothesis tests about components of the mediation model and is not based on an estimate of or formal inference about the indirect effect. In this paper we recast Judd et al.’s approach in the path-analytic framework that is now commonly used in between-participant mediation analysis. By so doing, it is apparent how to estimate the indirect effect of a within-participant manipulation on some outcome through a mediator as the product of paths of influence. This path analytic approach eliminates the need for discrete hypothesis tests about components of the model to support a claim of mediation, as Judd et al’s method requires, because it relies only on an inference about the product of paths— the indirect effect. We generalize methods of inference for the indirect effect widely used in between-participant designs to this within-participant version of mediation analysis, including bootstrap confidence intervals and Monte Carlo confidence intervals. Using this path analytic approach, we extend the method to models with multiple mediators operating in parallel and serially and discuss the comparison of indirect effects in these more complex models. We offer macros and code for SPSS, SAS, and Mplus that conduct these analyses.


Biometrika ◽  
1986 ◽  
Vol 73 (2) ◽  
pp. 333-343 ◽  
Author(s):  
JOHN T. KENT

Author(s):  
Ana Belén Ramos-Guajardo

AbstractA new clustering method for random intervals that are measured in the same units over the same group of individuals is provided. It takes into account the similarity degree between the expected values of the random intervals that can be analyzed by means of a two-sample similarity bootstrap test. Thus, the expectations of each pair of random intervals are compared through that test and a p-value matrix is finally obtained. The suggested clustering algorithm considers such a matrix where each p-value can be seen at the same time as a kind of similarity between the random intervals. The algorithm is iterative and includes an objective stopping criterion that leads to statistically similar clusters that are different from each other. Some simulations to show the empirical performance of the proposal are developed and the approach is applied to two real-life situations.


Geosciences ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 296
Author(s):  
Richard H. Groshong

This paper is a personal account of the origin and development of the twinned-calcite strain gauge, its experimental verification, and its relationship to stress analysis. The method allows the calculation of the three-dimensional deviatoric strain tensor based on five or more twin sets. A minimum of about 25 twin sets should provide a reasonably accurate result for the magnitude and orientation of the strain tensor. The opposite-signed strain axis orientation is the most accurately located. Where one strain axis is appreciably different from the other two, that axis is generally within about 10° of the correct value. Experiments confirm a magnitude accuracy of 1% strain over the range of 1–12% axial shortening and that samples with more than 40% negative expected values imply multiple or rotational deformations. If two deformations are at a high angle to one another, the strain calculated from the positive and negative expected values separately provides a good estimate of both deformations. Most stress analysis techniques do not provide useful magnitudes, although most provide a good estimate of the principal strain axis directions. Stress analysis based on the number of twin sets per grain provides a better than order-of-magnitude approximation to the differential stress magnitude in a constant strain rate experiment.


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